Cullin 7 (CUL7) (NM_001168370) Human Tagged ORF Clone
CAT#: RC230735
- TrueORF®
CUL7 (Myc-DDK-tagged)-Human cullin 7 (CUL7), transcript variant 1
ORF Plasmid: tGFP
"NM_001168370" in other vectors (2)
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CNY 11,976.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3M1; CUL-7; dJ20C7.5; KIAA0076 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC230735 representing NM_001168370
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGTGGCTTTTGGCTGGCGGAGCCGCTGGCAGGGACCGGGCCCCACCCTGCCCCAGTGGCTGCCG ACTCACGCGGGTGCAGCTCTGTTCCGCGGAGGCACGCCCCCTCCCGTCTCTCCGTCTCGACCCCCTCGCG GGGCCCAGGTGCCAGGATGGTGGGAGAACTCCGCTACAGGGAATTCAGGGTGCCCCTGGGGCCCGGCTTA CATGCCTATCCTGATGAGCTGATCCGCCAGCGCGTGGGCCATGATGGGCATCCTGAGTACCAGATCCGTT GGCTCATCCTGCGGCGTGGCGATGAGGGGGACGGGGGCTCTGGCCAAGTGGACTGCAAGGCTGAGCACAT CCTGCTGTGGATGTCCAAGGATGAGATCTATGCCAACTGCCACAAGATGCTGGGCGAGGATGGCCAGGTC ATCGGGCCCTCCCAGGAGTCTGCAGGGGAGGTTGGGGCCCTGGACAAATCTGTGCTGGAGGAGATGGAAA CCGACGTGAAGTCCCTCATTCAGAGAGCCCTTCGGCAGCTGGAGGAGTGTGTGGGCACTATCCCTCCTGC TCCTCTACTTCACACTGTCCACGTGCTCAGCGCCTATGCCAGCATTGAGCCCCTCACTGGAGTATTCAAG GACCCAAGGGTCCTGGACTTGCTCATGCACATGTTGAGTAGTCCTGATTATCAGATTCGCTGGAGTGCAG GCCGGATGATACAAGCCCTGTCCTCCCATGACGCTGGTGAGGGGCAGTGTGGGGAGGAAGGGAAAGCAGG AGAAGGGCTGGGTCGGCTCAGGGACTCACAGGACACTGTGGCAGGAGCCTCTGATCTCATCAGGACCCGG ACTCAGATCCTTCTGTCACTGAGCCAACAAGAAGCCATTGAGAAACACCTGGATTTTGACAGCCGCTGTG CTCTGCTAGCACTGTTTGCACAGGCCACGCTCTCTGAACACCCCATGTCTTTCGAGGGCATTCAGCTACC ACAGGTCCCAGGAAGGGTGCTCTTCTCCCTGGTGAAGCGGTATTTGCATGTCACCTCGCTCCTGGATCAG CTGAACGACAGTGCTGCGGAGCCAGGAGCCCAGAACACCTCTGCTCCTGAGGAGTTGAGTGGGGAGAGGG GTCAACTGGAGCTGGAGTTCAGTATGGCCATGGGCACCCTGATCTCGGAGCTGGTGCAAGCCATGCGCTG GGACCAGGCCTCAGACAGACCAAGGAGCTCAGCACGGTCCCCCGGTTCCATCTTCCAGCCTCAGCTGGCA GATGTGAGCCCAGGGCTCCCCGCTGCCCAGGCTCAGCCCTCCTTCAGGAGGTCAAGACGTTTTCGCCCTC GTTCTGAGTTCGCAAGTGGCAATACCTATGCTTTGTATGTGCGGGACACACTGCAGCCGGGGATGCGAGT GCGGATGCTGGATGATTATGAGGAGATCAGTGCCGGGGATGAGGGCGAGTTTCGGCAGAGCAACAACGGT GTGCCTCCTGTGCAGGTATTTTGGGAGTCAACAGGCCGCACCTATTGGGTGCACTGGCACATGCTGGAGA TCTTGGGCTTTGAGGAAGACATTGAGGACATGGTTGAGGCTGATGAGTACCAAGGGGCAGTGGCCAGTAG AGTCCTGGGTAGAGCCCTGCCTGCCTGGCGCTGGAGGCCCATGACAGAACTCTATGCTGTGCCTTATGTG CTGCCTGAGGATGAGGACACTGAGGAGTGTGAACACCTGACCCTGGCTGAGTGGTGGGAACTCCTCTTCT TCATCAAGAAGCTGGATGGACCTGACCATCAGGAGGTTCTCCAGATCCTCCAGGAGAACCTAGATGGGGA GATTCTGGATGATGAGATCCTAGCTGAACTGGCCGTGCCCATAGAATTGGCCCAGGACTTGCTGCTGACT CTGCCACAGCGACTCAATGACAGTGCCCTCAGGGACCTGATCAACTGCCATGTCTACAAGAAGTATGGGC CTGAAGCCCTAGCAGGGAACCAAGCCTACCCATCCCTTCTAGAAGCCCAAGAAGATGTCCTCCTGCTAGA CGCGCAGGCCCAGGCTAAGGACTCAGAAGATGCAGCCAAAGTGGAAGCAAAAGAACCCCCATCTCAGAGT CCCAACACTCCCCTGCAGCGTCTGGTGGAGGGTTATGGTCCAGCTGGGAAAATCCTCCTGGATCTAGAGC AAGCCCTCAGCTCAGAGGGGACCCAGGAGAACAAGGTCAAGCCACTCCTGCTGCAGCTGCAGCGGCAGCC GCAGCCCTTCCTGGCACTGATGCAGAGCCTGGACACTCCGGAGACTAACAGGACCCTGCACCTGACTGTG CTGAGAATCCTGAAGCAGCTGGTGGACTTCCCCGAGGCACTGCTGCTCCCCTGGCACGAGGCCGTGGATG CCTGCATGGCCTGCCTGCGGTCCCCAAACACTGATCGAGAGGTGCTCCAGGAACTGATTTTCTTCCTGCA CCGCCTGACCTCAGTGAGCAGGGACTATGCCGTGGTGCTGAATCAGCTGGGAGCAAGAGACGCTATCTCC AAGGCCCTGGAAAAGCACCTGGGAAAGCTGGAGCTGGCTCAGGAGCTGCGGGACATGGTGTTCAAGTGTG AGAAGCATGCCCACCTCTACCGCAAACTCATCACCAACATCCTGGGAGGCTGCATCCAGATGGTGCTGGG CCAGATCGAAGACCACAGACGAACCCACCAACCCATCAACATCCCTTTCTTTGATGTGTTCCTCAGATAC CTGTGCCAGGGCTCCAGTGTGGAAGTGAAGGAGGACAAGTGCTGGGAGAAGGTGGAGGTGTCCTCCAACC CGCACCGGGCCAGCAAGCTGACGGACCACAACCCCAAGACCTATTGGGAGTCCAACGGCAGCGCCGGCTC CCACTACATCACCCTGCACATGCGCCGGGGCATCCTCATCAGGCAACTGACTCTGCTTGTGGCTAGTGAG GACTCGAGTTACATGCCGGCCCGAGTGGTGGTGTGCGGGGGTGATAGCACTAGCTCTCTTCACACGGAAC TCAACTCGGTGAATGTGATGCCCTCTGCCAGCCGGGTGATCCTCCTGGAGAACCTGACCCGCTTCTGGCC CATCATCCAGATCCGCATAAAGCGCTGCCAGCAGGGTGGCATTGATACGCGCATTCGGGGGTTAGAGATC CTAGGCCCCAAGCCCACGTTCTGGCCAGTGTTCCGGGAGCAGCTCTGTCGTCACACACGCCTCTTCTACA TGGTTCGGGCACAGGCCTGGAGCCAGGACATGGCAGAGGACCGCAGGAGCCTCCTGCACCTGAGTTCTAG ACTCAACGGTGCTCTGCGCCAGGAGCAGAATTTTGCTGACCGCTTCCTCCCTGATGACGAGGCTGCCCAA GCTCTGGGCAAGACCTGCTGGGAGGCCCTGGTCAGCCCCGTGGTGCAGAACATCACCTCCCCTGATGAGG ATGGCATTAGCCCCCTGGGTTGGCTGCTGGACCAGTACCTGGAGTGTCAGGAAGCTGTCTTCAACCCCCA GAGCCGCGGCCCAGCTTTCTTCTCGCGGGTGCGCCGTCTCACTCACCTGCTGGTGCATGTCGAGCCCTGT GAGGCACCCCCTCCTGTGGTGGCCACTCCTCGGCCCAAAGGCAGAAACAGAAGCCACGACTGGAGCTCCT TGGCTACCCGGGGCCTTCCAAGCAGCATCATGAGAAACCTGACGCGCTGTTGGCGGGCCGTGGTGGAGAA GCAGGTGAACAATTTTCTGACCTCATCCTGGCGGGATGATGACTTTGTGCCACGCTACTGTGAGCACTTT AATATTCTGCAGAACTCAAGCTCTGAACTGTTTGGGCCTCGGGCAGCCTTCTTGCTGGCGCTGCAAAATG GCTGTGCGGGAGCCTTGCTGAAGCTCCCTTTTCTCAAAGCTGCCCACGTGAGTGAGCAGTTCGCCCGGCA CATTGACCAGCAGATCCAGGGCAGCCGGATCGGTGGAGCCCAGGAAATGGAGAGGCTGGCACAGCTGCAG CAATGCCTGCAAGCTGTCCTGATTTTCTCCGGCTTGGAGATAGCCACCACTTTTGAGCATTATTACCAGC ACTACATGGCGGACCGTCTCCTGGGCGTGGTCTCGAGCTGGCTGGAGGGGGCCGTGCTGGAGCAGATCGG TCCCTGCTTCCCCAACCGCCTCCCCCAGCAGATGTTGCAGAGCCTGAGCACCTCTAAGGAGCTGCAGCGC CAGTTCCACGTCTACCAGCTCCAGCAGCTGGATCAGGAACTCCTGAAGCTGGAGGATACAGAGAAGAAAA TACAGGTGGGCCTTGGGGCCAGTGGCAAGGAGCACAAGAGCGAGAAGGAAGAGGAAGCTGGGGCAGCAGC AGTGGTGGATGTGGCGGAGGGAGAGGAGGAAGAGGAGGAGAATGAGGACCTCTACTATGAAGGGGCAATG CCAGAAGTGTCTGTGCTTGTCCTGTCCCGACACTCCTGGCCTGTTGCCTCAATCTGCCACACACTGAACC CCAGAACCTGCCTGCCCTCCTACCTGAGGGGCACTTTGAACAGATACTCCAACTTCTACAACAAGAGTCA GAGCCACCCTGCCCTTGAGCGAGGCTCACAGAGGCGACTGCAGTGGACGTGGCTGGGCTGGGCTGAGCTG CAGTTTGGGAACCAGACCCTGCATGTGTCCACCGTGCAGATGTGGCTACTGCTGTATCTCAACGACCTGA AGGCGGTCTCTGTGGAGAGTCTGCTGGCGTTCTCAGGGCTCTCCGCAGACATGCTCAATCAGGCGATTGG GCCCCTCACCTCTTCAAGAGGCCCCCTGGACCTTCACGAGCAAAAGGATATACCAGGAGGGGTCCTCAAG ATTCGAGATGGCAGCAAGGAACCCAGGTCGAGATGGGACATTGTGCGGCTCATCCCACCTCAGACGTACC TGCAAGCTGAGGGTGAAGACGGCCAGAACTTGGAGAAGAGACGGAATCTTCTGAACTGCCTCATCGTCCG AATCCTCAAGGCCCATGGAGATGAGGGGCTGCACATTGACCAGCTTGTCTGTCTGGTGCTGGAGGCTTGG CAGAAGGGCCCGTGTCCTCCCAGGGGTTTGGTCAGCAGCCTTGGTAAGGGGTCTGCATGCAGCAGCACTG ACGTCCTCTCCTGCATCCTACACCTCCTGGGCAAGGGCACGCTGAGACGCCATGACGACCGGCCCCAGGT GCTGTCCTATGCAGTCCCTGTGACTGTCATGGAGCCTCACACTGAGTCCCTGAACCCAGGCTCCTCAGGC CCCAACCCACCCCTCACCTTCCATACCCTACAGATTCGCTCCCGGGGTGTGCCCTATGCCTCCTGCACTG CCACCCAGAGCTTCTCTACCTTCCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230735 representing NM_001168370
Red=Cloning site Green=Tags(s) MSRGFWLAEPLAGTGPHPAPVAADSRGCSSVPRRHAPSRLSVSTPSRGPGARMVGELRYREFRVPLGPGL HAYPDELIRQRVGHDGHPEYQIRWLILRRGDEGDGGSGQVDCKAEHILLWMSKDEIYANCHKMLGEDGQV IGPSQESAGEVGALDKSVLEEMETDVKSLIQRALRQLEECVGTIPPAPLLHTVHVLSAYASIEPLTGVFK DPRVLDLLMHMLSSPDYQIRWSAGRMIQALSSHDAGEGQCGEEGKAGEGLGRLRDSQDTVAGASDLIRTR TQILLSLSQQEAIEKHLDFDSRCALLALFAQATLSEHPMSFEGIQLPQVPGRVLFSLVKRYLHVTSLLDQ LNDSAAEPGAQNTSAPEELSGERGQLELEFSMAMGTLISELVQAMRWDQASDRPRSSARSPGSIFQPQLA DVSPGLPAAQAQPSFRRSRRFRPRSEFASGNTYALYVRDTLQPGMRVRMLDDYEEISAGDEGEFRQSNNG VPPVQVFWESTGRTYWVHWHMLEILGFEEDIEDMVEADEYQGAVASRVLGRALPAWRWRPMTELYAVPYV LPEDEDTEECEHLTLAEWWELLFFIKKLDGPDHQEVLQILQENLDGEILDDEILAELAVPIELAQDLLLT LPQRLNDSALRDLINCHVYKKYGPEALAGNQAYPSLLEAQEDVLLLDAQAQAKDSEDAAKVEAKEPPSQS PNTPLQRLVEGYGPAGKILLDLEQALSSEGTQENKVKPLLLQLQRQPQPFLALMQSLDTPETNRTLHLTV LRILKQLVDFPEALLLPWHEAVDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAIS KALEKHLGKLELAQELRDMVFKCEKHAHLYRKLITNILGGCIQMVLGQIEDHRRTHQPINIPFFDVFLRY LCQGSSVEVKEDKCWEKVEVSSNPHRASKLTDHNPKTYWESNGSAGSHYITLHMRRGILIRQLTLLVASE DSSYMPARVVVCGGDSTSSLHTELNSVNVMPSASRVILLENLTRFWPIIQIRIKRCQQGGIDTRIRGLEI LGPKPTFWPVFREQLCRHTRLFYMVRAQAWSQDMAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQ ALGKTCWEALVSPVVQNITSPDEDGISPLGWLLDQYLECQEAVFNPQSRGPAFFSRVRRLTHLLVHVEPC EAPPPVVATPRPKGRNRSHDWSSLATRGLPSSIMRNLTRCWRAVVEKQVNNFLTSSWRDDDFVPRYCEHF NILQNSSSELFGPRAAFLLALQNGCAGALLKLPFLKAAHVSEQFARHIDQQIQGSRIGGAQEMERLAQLQ QCLQAVLIFSGLEIATTFEHYYQHYMADRLLGVVSSWLEGAVLEQIGPCFPNRLPQQMLQSLSTSKELQR QFHVYQLQQLDQELLKLEDTEKKIQVGLGASGKEHKSEKEEEAGAAAVVDVAEGEEEEEENEDLYYEGAM PEVSVLVLSRHSWPVASICHTLNPRTCLPSYLRGTLNRYSNFYNKSQSHPALERGSQRRLQWTWLGWAEL QFGNQTLHVSTVQMWLLLYLNDLKAVSVESLLAFSGLSADMLNQAIGPLTSSRGPLDLHEQKDIPGGVLK IRDGSKEPRSRWDIVRLIPPQTYLQAEGEDGQNLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAW QKGPCPPRGLVSSLGKGSACSSTDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPHTESLNPGSSG PNPPLTFHTLQIRSRGVPYASCTATQSFSTFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001168370 |
ORF Size | 5346 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001168370.1, NP_001161842.1 |
RefSeq ORF | 5193 bp |
Locus ID | 9820 |
UniProt ID | Q14999 |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 200.2 kDa |
Gene Summary | The protein encoded by this gene is a component of an E3 ubiquitin-protein ligase complex. The encoded protein interacts with TP53, CUL9, and FBXW8 proteins. Defects in this gene are a cause of 3M syndrome type 1 (3M1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009] |
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