ZNF548 (NM_001172773) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001172773, the custom clone sequence may differ by one or more nucleotides ATGAACCTGACTGAGGGTCCCCTGGCGATGGCAGAAATGGACCCTACACAGGGCCGTGTG GTCTTTGAGGACGTGGCCATATATTTCTCCCAGGAGGAGTGGGGGCACCTTGATGAGGCT CAGAGATTGCTGTACCGTGATGTGATGCTGGAGAATTTGGCCCTTTTGTCCTCACTAGGT TCTTGGCATGGAGCTGAGGATGAGGAGGCACCTTCACAGCAAGGTTTTTCTGTAGGAGTG TCAGAGGTTACAGCTTCAAAGCCCTGTCTGTCCAGCCAGAAGGTCCACCCTAGTGAGACA TGTGGCCCACCCTTGAAAGACATTCTGTGCCTGGTTGAGCACAATGGAATTCATCCTGAG CAACACATATATATTTGTGAGGCAGAGCTTTTTCAGCACCCAAAGCAGCAAATTGGAGAA AATCTTTCCAGAGGGGATGATTGGATACCTTCATTTGGGAAGAACCACAGAGTTCACATG GCAGAGGAGATCTTCACATGCATGGAGGGCTGGAAGGACTTACCAGCCACCTCATGCCTT CTCCAGCACCAGGGCCCTCAAAGCGAGTGGAAGCCATACAGGGACACAGAGGACAGAGAA GCCTTTCAGACTGGACAAAATGATTACAAATGTAGTGAATGTGGGAAAACCTTCACCTGC AGCTATTCATTTGTTGAGCACCAGAAAATCCACACAGGAGAAAGGTCTTATGAATGTAAC AAATGTGGGAAATTCTTTAAGTACAGTGCCAATTTCATGAAACATCAGACAGTTCACACT AGTGAAAGGACTTATGAGTGCAGAGAATGTGGAAAATCCTTTATGTACAACTACCGACTC ATGAGACATAAGCGAGTTCACACTGGAGAAAGGCCTTATGAGTGCAACACATGTGGGAAA TTCTTTCGGTACAGCTCCACATTTGTTAGACATCAGAGAGTTCACACCGGAGAAAGGCCG TATGAGTGCAGGGAATGTGGGAAATTCTTTATGGACAGCTCCACACTCATTAAACATCAG AGAGTTCACACCGGAGAAAGACCTTATAAGTGCAATGATTGTGGGAAATTTTTTAGGTAT ATCTCCACACTCATTAGACATCAGAGAATTCACACTGGAGAAAGGCCTTATGAGTGCAGT GTATGTGGGGAATTGTTTAGGTACAACTCCAGCCTTGTTAAACATTGGAGAAATCACACT GGAGAAAGGCCTTATAAATGCAGTGAATGTGGGAAATCATTTAGGTACCACTGCAGGCTC ATTAGACACCAGAGAGTCCACACGGGAGAAAGGCCTTATGAGTGCAGCGAATGCGGGAAA TTCTTTCGTTACAACTCCAACCTCATTAAACATTGGAGAAATCACACTGGAGAAAGGCCT TACGAGTGCAGAGAGTGTGGGAAAGCCTTTAGCCACAAGCATATACTTGTTGAGCACCAG AAAATCCACAGTGGAGAAAGACCTTATGAGTGCAGCGAATGCCAGAAGGCCTTTATTAGA AAGTCTCACCTGGTTCATCACCAGAAAATCCACAGTGAAGAGAGGCTTGTGTGCTCCATG AATGTGGGGAATTCTTTAGCTAAAACTCCAACCTCATTAAACATCAGAGATTTCACAATG GAGAAAGTTTACCATTGA
Restriction Sites	Please inquire
ACCN	NM_001172773
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001172773.1, NP_001166244.1
RefSeq Size	4565 bp
RefSeq ORF	1638 bp
Locus ID	147694
UniProt ID	Q8NEK5
Protein Families	Transcription Factors
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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