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ZNF548 (NM_001172773) Human Tagged ORF Clone
CAT＃: RG230255
- TrueORF®
ZNF548 (tGFP-tagged) - Human zinc finger protein 548 (ZNF548), transcript variant 1. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001172773" in other vectors (2)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230255 ORF sequence, codon optimized.
Due to the complexity of NM_001172773, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTCACTGAGGGTCCGCTTGCCATGGCCGAGATGGATCCTACACAGGGTAGGGTGGTTTTCGAAG ATGTGGCCATCTATTTTTCACAGGAAGAGTGGGGGCACCTTGACGAGGCACAGCGACTGCTGTATCGGGA CGTGATGCTCGAGAATTTGGCCCTCCTGAGTTCTCTTGGGTCCTGGCATGGGGCTGAAGATGAGGAGGCT CCTAGTCAACAAGGTTTTTCTGTAGGAGTTTCTGAGGTGACGGCCTCCAAGCCATGCCTGTCATCTCAAA AGGTGCATCCGTCTGAGACGTGTGGGCCACCTTTGAAAGATATTTTGTGTCTGGTGGAACATAATGGGAT TCACCCCGAGCAGCACATCTATATTTGTGAAGCTGAGTTGTTTCAGCACCCGAAGCAGCAGATCGGTGAG AATCTGTCTCGCGGGGACGATTGGATTCCCAGTTTTGGTAAGAATCACCGCGTTCACATGGCCGAGGAAA TTTTTACCTGTATGGAGGGGTGGAAGGACCTTCCAGCTACATCTTGCCTGCTCCAGCACCAGGGACCACA ATCAGAGTGGAAGCCCTATCGCGATACAGAGGATAGGGAAGCCTTCCAGACCGGCCAAAACGACTACAAG TGCTCTGAATGCGGCAAAACCTTTACTTGCTCATACTCTTTCGTCGAGCACCAGAAGATCCACACGGGGG AGCGGTCCTATGAGTGTAACAAATGCGGTAAATTTTTTAAGTACTCCGCTAATTTCATGAAGCACCAGAC TGTACACACATCTGAACGGACCTATGAGTGCCGGGAATGCGGTAAGAGCTTCATGTATAATTACCGCCTC ATGAGACATAAGCGAGTCCACACCGGGGAGAGGCCATATGAGTGCAATACCTGTGGCAAATTCTTTCGAT ACAGTTCTACATTTGTGCGCCATCAAAGAGTGCATACGGGAGAGCGCCCTTACGAGTGTAGAGAATGCGG AAAGTTCTTTATGGATTCATCCACCTTGATTAAGCACCAGCGAGTGCACACAGGCGAACGGCCATACAAA TGCAATGACTGTGGAAAGTTCTTTAGGTATATTTCTACGCTGATCAGGCACCAAAGAATTCACACCGGCG AAAGGCCTTACGAGTGCAGCGTTTGTGGCGAACTGTTCAGATATAACTCATCTTTGGTGAAGCACTGGCG CAACCATACCGGCGAAAGACCTTATAAATGCTCTGAGTGCGGCAAAAGTTTCAGGTATCACTGTCGACTG ATCAGACACCAAAGAGTTCACACCGGTGAAAGGCCGTACGAGTGCAGCGAGTGTGGTAAGTTTTTCAGAT ATAACTCAAACCTTATCAAACACTGGAGGAATCATACAGGGGAGAGGCCTTATGAATGTCGCGAGTGTGG TAAAGCTTTCAGCCACAAGCACATCCTCGTGGAGCACCAGAAGATCCACAGTGGAGAACGCCCTTATGAA TGTTCCGAGTGCCAGAAAGCCTTTATTCGGAAATCCCACTTGGTCCACCACCAGAAGATTCACTCCGAAG AAAGACTCGTATGTTCCATGAATGTAGGCAATAGCCTTGCCAAAACTCCAACTTCCCTGAACATCAGAGA TTTCACCATGGAGAAGGTCTACCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230255 representing NM_001172773
Red=Cloning site Green=Tags(s) MNLTEGPLAMAEMDPTQGRVVFEDVAIYFSQEEWGHLDEAQRLLYRDVMLENLALLSSLGSWHGAEDEEA PSQQGFSVGVSEVTASKPCLSSQKVHPSETCGPPLKDILCLVEHNGIHPEQHIYICEAELFQHPKQQIGE NLSRGDDWIPSFGKNHRVHMAEEIFTCMEGWKDLPATSCLLQHQGPQSEWKPYRDTEDREAFQTGQNDYK CSECGKTFTCSYSFVEHQKIHTGERSYECNKCGKFFKYSANFMKHQTVHTSERTYECRECGKSFMYNYRL MRHKRVHTGERPYECNTCGKFFRYSSTFVRHQRVHTGERPYECRECGKFFMDSSTLIKHQRVHTGERPYK CNDCGKFFRYISTLIRHQRIHTGERPYECSVCGELFRYNSSLVKHWRNHTGERPYKCSECGKSFRYHCRL IRHQRVHTGERPYECSECGKFFRYNSNLIKHWRNHTGERPYECRECGKAFSHKHILVEHQKIHSGERPYE CSECQKAFIRKSHLVHHQKIHSEERLVCSMNVGNSLAKTPTSLNIRDFTMEKVYH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001172773 |
| ORF Size | 1635 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172773.1, NP_001166244.1 |
| RefSeq Size | 4987 bp |
| RefSeq ORF | 1638 bp |
| Locus ID | 147694 |
| UniProt ID | Q8NEK5 |
| Protein Families | Transcription Factors |
| Gene Summary | May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
