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ANKRD32 (SLF1) (NM_032290) Human Untagged Clone
CAT＃: SC328043
ANKRD32 (untagged)-Human ankyrin repeat domain 32 (ANKRD32)
CNY 20,430.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANKRD32; BRCTD1; BRCTx |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328043 representing NM_032290.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGATGGTACCCCAAAGCATATCATCCAGATGACAGGATTTAAGATGGAAGAAAAAGAAGCGCTA GTCAAATTACTTTTAAAACTAGATTGCACTTTTATTAAGAGTGAGAAATACAAAAATTGTACACATCTT ATAGCTGAACGCCTATGTAAGAGTGAAAAATTTTTAGCAGCTTGTGCGGCAGGAAAGTGGATACTAACC AAGGACTATATAATTCATAGTGCCAAAAGTGGCAGATGGCTTGATGAAACAACTTATGAATGGGGATAT AAAATTGAAAAAGATTCCCGTTATTCACCTCAAATGCAATCTGCACCTAAAAGATGGCGTGAAGAACTG AAACGCACTGGTGCTCCAGGAGCCTTCCACAGATGGAAAGTTGTCCTCCTTGTTAGAACTGATAAGCGA AGTGATTCTCTTATAAGAGTTTTGGAGGCTGGAAAGGCAAATGTTATTTTACCAAAAAGTTCACCAAGT GGAATAACTCATGTGATTGCCAGTAATGCAAGAATTAAAGCTGAGAAAGAAAAAGATAACTTTAAGGCT CCATTTTATCCAATTCAGTATCTAGGGGATTTTCTTTTAGAGAAAGAAATTCAGAATGATGAAGATTCC CAAACCAATTCTGTTTGGACTGAACATAGCAATGAAGAAACAAACAAAGATTTCAGGAAAGATGCAGGA TTTCTTGAAATGAAAGGTGCCTTAAGAGAGACCATGTATAGAACCCAGAAAGAAATGCAAAATCATGAA GATGTTAATGTTGGTTCTATTTTGATTCAACATCACAAAAAAGAAAAATTCAGTGGTTCCAGTAAAGAT TTGAAATTTGTTAAAATGAGAAATACCTTTGGAAGCCATACATATGAAAATCAGAAGGAAATTAAGAAA AAAGATGAAGATATTCAGAGGAGTTATACTTTGAGGAGAAAACGCAAGAAAGGAAAAGAAAGCAATTGC AAGAAAGGCGTTGAACATGAAAAAATAAAAAGTACCTTAAGAAGGCACATATATAATAGAGATCAGAAA GAAATGAAGAATTCTATTTTTGCTGAATATGCCAAAGAATCAAAAGCCATGGCTATTAAGACAGATGTG GATGTTGTTGAAATAAAAAATACCTTAAGGAAGCACATATATAGAGCTCAGGCTGTCAGATACAACTGC ATTAGAATAGATAAACAACCAGTGTACAACGTAGAGGTTAAAAATGCTGAATTTCCCAGAGGTGTATTA AATTTAATTGAAAGCCTCATAGAAGGACATTTTTTTAAAGAAGCAATTGAGGAACTTTCCACTTTGCAG GCACATTATATCCCTCCTGTATGCGTTCTTCATGCCCTTCTAGAGAACGTTCTACAGGATAACATAGAT ACATTTTCTGGTCGATACTTTCATATATTGTCAGCTCTTCTTCACCTGCATCCTCCTTGGAAGTCTCCA GCCATGTCGAGATATTATTTAGAGTTGTTTCAGTGTCCAACTTGTATGAAAGGAGCATGGTCTTTAGTA GAAGTCCTTATCAGGTCTTGCCTTTTCAATGAAAGCTTTTGTCATCAAATTTCAGAAAATATTGGCTCC AAGGTGCTCCACCTGACGCTACTCAAATTTTTCTTTAATTTAATTGAAAGTGAAGTACAACATCTGAGT CAAAAGTTGTATGACTGGTCAGATTCTCAGAATCTGAAAATAACAGGAAAGGCAATGCTTCTTGAAATT TTTTGGTCAGGAAGTGAAACCTCTGGGCTTTTGACCAAACCAGTAAATATGCTTTTGGAATGGACTATA TATTCTCACAAGGAAAAATTCAAGTCTAATGATGTCTTCAAACATGAACTAGCTTACTTATTGGCTGGA ATTCTTGGAGCAGCAATAGATTATTGGATTTTCCTTGGTCTTAAGATGGGTAGAAATGTGATGCGACAC ATGTCTGATGACTTAGGAAGTTATGTTTCTCTTTCGTGTGATGACTTTTCTTCACAGGAATTAGAGATT TTCATTTGCTCCTTTTCCTCCTCCTGGCTTCAAATGTTTGTTGCAGAGGCAGTCTTTAAAAAGTTGTGT CTACAGAGCTCTGGCAGTGTTTCTTCTGAGCCACTCTCTCTTCAGAAAATGGTATATTCCTATTTACCA GCCTTGGGGAAAACTGGTGTGCTTGGGTCTGGAAAGATTCAGGTGTCAAAGAAAATAGGACAGCGGCCT TGTTTTGACTCTCAGAGAACCTTACTAATGCTGAATGGTACTAAACAAAAACAAGTCGAAGGGCTGCCA GAGTTACTAGACCTGAACCTTGCTAAATGTTCCTCATCATTAAAAAAATTGAAAAAGAAGTCAGAAGGA GAATTGTCATGTTCCAAGGAGAATTGCCCCTCTGTAGTTAAAAAGATGAATTTTCACAAGACTAATCTA AAAGGAGAAACAGCCCTGCATAGAGCTTGCATAAATAACCAAGTGGAGAAATTGATTCTTCTTCTCTCT TTGCCAGGAATAGACATCAATGTTAAAGACAATGCTGGCTGGACGCCTTTGCATGAAGCCTGTAACTAT GGCAACACAGTGTGTGTCCAGGAAATTTTGCAACGTTGTCCAGAGGTAGATCTGCTCACTCAAGTGGAC GGGGTGACTCCTTTGCATGATGCACTGTCAAACGGACATGTAGAAATTGGCAAGCTGCTACTACAGCAT GGGGGCCCAGTGCTTTTACAACAGAGGAATGCTAAGGGAGAATTGCCCTTGGATTATGTGGTTTCACCT CAAATCAAAGAAGAACTGTTTGCTATTACAAAAATAGAAGATACAGTGGAGAACTTTCATGCACAAGCA GAGAAACATTTTCATTACCAGCAACTTGAATTTGGCTCCTTTTTACTTAGTAGGATGTTGCTAAATTTT TGTTCAATTTTTGATTTATCTTCAGAGTTCATTTTAGCTTCCAAAGGGTTAACTCATCTAAATGAACTG CTTATGGCTTGTAAAAGTCATAAAGAAACCACCAGTGTTCATACTGACTGGTTACTGGATCTTTATGCT GGAAATATAAAGACATTGCAGAAACTCCCACACATTCTTAAGGAACTGCCTGAGAATTTGAAAGTGTGT CCTGGGGTACACACTGAGGCCTTGATGATAACATTGGAAATGATGTGTCGGTCAGTCATGGAGTTTTCA TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_032290 |
| Insert Size | 3177 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032290.3 |
| RefSeq Size | 3813 bp |
| RefSeq ORF | 3177 bp |
| Locus ID | 84250 |
| UniProt ID | Q9BQI6 |
| MW | 121.1 kDa |
| Gene Summary | Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions (PubMed:25931565).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209491 | ANKRD32 (Myc-DDK-tagged)-Human ankyrin repeat domain 32 (ANKRD32) |
CNY 3,656.00 |
|
| RC209491L3 | Lenti ORF clone of Human ankyrin repeat domain 32 (ANKRD32), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC209491L4 | Lenti ORF clone of Human ankyrin repeat domain 32 (ANKRD32), mGFP tagged |
CNY 10,260.00 |
|
| RG209491 | ANKRD32 (tGFP-tagged) - Human ankyrin repeat domain 32 (ANKRD32) |
CNY 5,256.00 |
|
| SC305430 | ANKRD32 (untagged)-Human ankyrin repeat domain 32 (ANKRD32) |
CNY 20,430.00 |
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