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ANKRD32 (SLF1) (NM_032290) Human Tagged ORF Clone
CAT＃: RC209491
- TrueORF®
ANKRD32 (Myc-DDK-tagged)-Human ankyrin repeat domain 32 (ANKRD32)
ORF Plasmid: tGFP
"NM_032290" in other vectors (5)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ANKRD32; BRCTD1; BRCTx |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209491 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTAGAAATGTGATGCGACACATGTCTGATGACTTAGGAAGTTATGTTTCTCTTTCGTGTGATGACT TTTCTTCACAGGAATTAGAGATTTTCATTTGCTCCTTTTCCTCCTCCTGGCTTCAAATGTTTGTTGCAGA GGCAGTCTTTAAAAAGTTGTGTCTACAGAGCTCTGGCAGTGTTTCTTCTGAGCCACTCTCTCTTCAGAAA ATGGTATATTCCTATTTACCAGCCTTGGGGAAAACTGGTGTGCTTGGGTCTGGAAAGATTCAGGTGTCAA AGAAAATAGGACAGCGGCCTTGTTTTGACTCTCAGAGAACCTTACTAATGCTGAATGGTACTAAACAAAA ACAAGTCGAAGGGCTGCCAGAGTTACTAGACCTGAACCTTGCTAAATGTTCCTCATCATTAAAAAAATTG AAAAAGAAGTCAGAAGGAGAATTGTCATGTTCCAAGGAGAATTGCCCCTCTGTAGTTAAAAAGATGAATT TTCACAAGACTAATCTAAAAGGAGAAACAGCCCTGCATAGAGCTTGCATAAATAACCAAGTGGAGAAATT GATTCTTCTTCTCTCTTTGCCAGGAATAGACATCAATGTTAAAGACAATGCTGGCTGGACGCCTTTGCAT GAAGCCTGTAACTATGGCAACACAGTGTGTGTCCAGGAAATTTTGCAACGTTGTCCAGAGGTAGATCTGC TCACTCAAGTGGACGGGGTGACTCCTTTGCATGATGCACTGTCAAACGGACATGTAGAAATTGGCAAGCT GCTACTACAGCATGGGGGCCCAGTGCTTTTACAACAGAGGAATGCTAAGGGAGAATTGCCCTTGGATTAT GTGGTTTCACCTCAAATCAAAGAAGAACTGTTTGCTATTACAAAAATAGAAGATACAGTGGAGAACTTTC ATGCACAAGCAGAGAAACATTTTCATTACCAGCAACTTGAATTTGGCTCCTTTTTACTTAGTAGGATGTT GCTAAATTTTTGTTCAATTTTTGATTTATCTTCAGAGTTCATTTTAGCTTCCAAAGGGTTAACTCATCTA AATGAACTGCTTATGGCTTGTAAAAGTCATAAAGAAACCACCAGTGTTCATACTGACTGGTTACTGGATC TTTATGCTGGAAATATAAAGACATTGCAGAAACTCCCACACATTCTTAAGGAACTGCCTGAGAATTTGAA AGTGTGTCCTGGGGTACACACTGAGGCCTTGATGATAACATTGGAAATGATGTGTCGGTCAGTCATGGAG TTTTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209491 protein sequence
Red=Cloning site Green=Tags(s) MGRNVMRHMSDDLGSYVSLSCDDFSSQELEIFICSFSSSWLQMFVAEAVFKKLCLQSSGSVSSEPLSLQK MVYSYLPALGKTGVLGSGKIQVSKKIGQRPCFDSQRTLLMLNGTKQKQVEGLPELLDLNLAKCSSSLKKL KKKSEGELSCSKENCPSVVKKMNFHKTNLKGETALHRACINNQVEKLILLLSLPGIDINVKDNAGWTPLH EACNYGNTVCVQEILQRCPEVDLLTQVDGVTPLHDALSNGHVEIGKLLLQHGGPVLLQQRNAKGELPLDY VVSPQIKEELFAITKIEDTVENFHAQAEKHFHYQQLEFGSFLLSRMLLNFCSIFDLSSEFILASKGLTHL NELLMACKSHKETTSVHTDWLLDLYAGNIKTLQKLPHILKELPENLKVCPGVHTEALMITLEMMCRSVME FS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032290 |
| ORF Size | 1266 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032290.2, NP_115666.1 |
| RefSeq Size | 3813 bp |
| RefSeq ORF | 3177 bp |
| Locus ID | 84250 |
| UniProt ID | Q9BQI6 |
| MW | 47.1 kDa |
| Gene Summary | Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions (PubMed:25931565).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209491L3 | Lenti ORF clone of Human ankyrin repeat domain 32 (ANKRD32), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC209491L4 | Lenti ORF clone of Human ankyrin repeat domain 32 (ANKRD32), mGFP tagged |
CNY 10,260.00 |
|
| RG209491 | ANKRD32 (tGFP-tagged) - Human ankyrin repeat domain 32 (ANKRD32) |
CNY 5,256.00 |
|
| SC305430 | ANKRD32 (untagged)-Human ankyrin repeat domain 32 (ANKRD32) |
CNY 20,430.00 |
|
| SC328043 | ANKRD32 (untagged)-Human ankyrin repeat domain 32 (ANKRD32) |
CNY 20,430.00 |
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