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MID2 (NM_012216) Human Untagged Clone
CAT＃: SC327991
MID2 (untagged)-Human midline 2 (MID2) transcript variant 1
CNY 14,060.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FXY2; MRX101; RNF60; TRIM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327991 representing NM_012216.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGTGAAAGCCCAGCCTCCGTGGTTCTTAATGCCTCAGGAGGACTATTTTCACTAAAGATGGAAACA CTGGAGTCTGAATTGACCTGTCCAATCTGCCTAGAGTTGTTTGAAGACCCCCTTCTGCTCCCTTGTGCT CACAGCCTCTGCTTCAGCTGTGCCCATCGCATTTTGGTATCAAGCTGCAGCTCTGGTGAATCCATTGAA CCCATTACTGCTTTCCAGTGTCCTACCTGCAGGTATGTTATCTCGCTGAACCACCGGGGCCTGGATGGC CTCAAGAGGAATGTGACTCTGCAGAACATTATTGATCGCTTCCAGAAGGCTTCAGTCAGTGGGCCCAAT TCCCCTAGTGAGAGCCGCCGGGAAAGGACTTACAGGCCCACCACTGCCATGTCTAGCGAGCGAATTGCT TGCCAATTCTGTGAGCAGGACCCGCCAAGGGATGCAGTAAAAACATGCATCACCTGTGAGGTCTCCTAC TGTGACCGTTGCCTGCGGGCCACGCACCCCAACAAGAAACCTTTCACCAGCCACCGCCTGGTGGAACCA GTGCCAGACACACATCTTCGAGGGATCACCTGCCTGGACCATGAGAATGAGAAAGTGAACATGTACTGT GTATCTGATGACCAATTGATCTGTGCCTTATGCAAACTGGTGGGTCGTCACCGAGACCATCAGGTCGCA TCCCTGAATGATCGATTTGAGAAACTCAAGCAAACTCTGGAGATGAACCTCACCAACCTGGTTAAGCGC AACAGCGAACTAGAAAATCAAATGGCCAAACTAATACAGATCTGCCAGCAGGTTGAGGTGAATACTGCT ATGCATGAGGCAAAACTTATGGAAGAATGTGACGAGTTGGTAGAGATCATCCAGCAGAGGAAGCAAATG ATCGCTGTCAAAATCAAAGAGACAAAGGTTATGAAACTGAGAAAGTTGGCACAGCAGGTTGCTAATTGC CGCCAGTGTCTTGAACGGTCAACAGTCCTCATCAACCAAGCTGAGCATATCCTGAAAGAAAATGACCAG GCACGGTTTCTACAGTCTGCAAAAAATATTGCTGAGAGGGTCGCTATGGCAACTGCATCTTCTCAAGTT CTGATTCCAGACATCAATTTTAATGATGCCTTTGAAAACTTTGCTTTAGATTTTTCCAGAGAAAAGAAA CTGCTAGAGGGGTTAGATTATTTAACAGCCCCAAACCCACCATCTATCCGAGAAGAACTCTGTACTGCC TCCCATGACACCATTACAGTCCACTGGATCTCGGATGATGAGTTCAGCATCAGCTCCTATGAGCTTCAG TACACCATATTCACTGGCCAGGCTAACTTCATCAGTAAGTCATGGTGTAGTTGGGGCCTGTGGCCAGAG ATAAGGAAATGTAAGGAAGCAGTAAGCTGCTCAAGATTGGCCGGGGCGCCACGAGGCCTGTATAATTCA GTAGACAGCTGGATGATTGTTCCCAACATTAAACAGAACCATTACACAGTGCATGGACTCCAGAGCGGG ACTCGCTACATCTTCATCGTTAAAGCCATAAACCAAGCCGGCAGCCGGAACAGTGAACCTACCCGACTA AAAACAAACAGCCAACCCTTTAAATTGGATCCCAAAATGACTCACAAGAAGTTGAAGATCTCCAATGAT GGATTGCAGATGGAGAAGGATGAAAGCTCTCTAAAGAAGAGCCACACCCCAGAGAGGTTTAGTGGCACA GGGTGCTATGGGGCAGCAGGAAATATATTCATTGACAGTGGCTGCCACTATTGGGAGGTGGTCATGGGT TCCTCAACATGGTATGCAATTGGCATTGCCTACAAATCAGCTCCAAAGAATGAATGGATTGGCAAGAAT GCCTCCTCATGGGTCTTCTCTCGCTGCAATAGTAACTTCGTGGTGAGACACAACAACAAGGAAATGCTG GTGGATGTGCCCCCACACCTGAAGCGTCTGGGTGTCCTCCTGGATTATGACAACAATATGCTGTCTTTC TATGACCCAGCTAACTCTCTCCATCTTCATACTTTTGATGTGACCTTCATTCTTCCAGTTTGTCCAACA TTTACAATCTGGAACAAATCCCTAATGATCCTGTCTGGCTTGCCTGCCCCAGATTTTATTGATTACCCT GAGCGGCAGGAATGCAACTGCAGGCCTCAAGAATCCCCTTATGTTTCTGGGATGAAAACCTGTCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012216 |
| Insert Size | 2208 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012216.3 |
| RefSeq Size | 7345 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 11043 |
| UniProt ID | Q9UJV3 |
| Domains | zf-B_box, RING, BBC, SPRY, FN3 |
| Protein Families | Druggable Genome |
| MW | 83.2 kDa |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229356 | MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 5,384.00 |
|
| RC229356L3 | Lenti-ORF clone of MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 7,700.00 |
|
| RC229356L4 | Lenti-ORF clone of MID2 (mGFP-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 7,700.00 |
|
| RG229356 | MID2 (tGFP-tagged) - Human midline 2 (MID2), transcript variant 1 |
CNY 6,370.00 |
|
| SC115493 | MID2 (untagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 5,248.00 |
