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MID2 (NM_012216) Human Tagged ORF Clone
CAT＃: RG229356
- TrueORF®
MID2 (tGFP-tagged) - Human midline 2 (MID2), transcript variant 1
ORF Plasmid: DDK
"NM_012216" in other vectors (5)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FXY2; MRX101; RNF60; TRIM1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229356 representing NM_012216
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGAAAGCCCAGCCTCCGTGGTTCTTAATGCCTCAGGAGGACTATTTTCACTAAAGATGGAAACAC TGGAGTCTGAATTGACCTGTCCAATCTGCCTAGAGTTGTTTGAAGACCCCCTTCTGCTCCCTTGTGCTCA CAGCCTCTGCTTCAGCTGTGCCCATCGCATTTTGGTATCAAGCTGCAGCTCTGGTGAATCCATTGAACCC ATTACTGCTTTCCAGTGTCCTACCTGCAGGTATGTTATCTCGCTGAACCACCGGGGCCTGGATGGCCTCA AGAGGAATGTGACTCTGCAGAACATTATTGATCGCTTCCAGAAGGCTTCAGTCAGTGGGCCCAATTCCCC TAGTGAGAGCCGCCGGGAAAGGACTTACAGGCCCACCACTGCCATGTCTAGCGAGCGAATTGCTTGCCAA TTCTGTGAGCAGGACCCGCCAAGGGATGCAGTAAAAACATGCATCACCTGTGAGGTCTCCTACTGTGACC GTTGCCTGCGGGCCACGCACCCCAACAAGAAACCTTTCACCAGCCACCGCCTGGTGGAACCAGTGCCAGA CACACATCTTCGAGGGATCACCTGCCTGGACCATGAGAATGAGAAAGTGAACATGTACTGTGTATCTGAT GACCAATTGATCTGTGCCTTATGCAAACTGGTGGGTCGTCACCGAGACCATCAGGTCGCATCCCTGAATG ATCGATTTGAGAAACTCAAGCAAACTCTGGAGATGAACCTCACCAACCTGGTTAAGCGCAACAGCGAACT AGAAAATCAAATGGCCAAACTAATACAGATCTGCCAGCAGGTTGAGGTGAATACTGCTATGCATGAGGCA AAACTTATGGAAGAATGTGACGAGTTGGTAGAGATCATCCAGCAGAGGAAGCAAATGATCGCTGTCAAAA TCAAAGAGACAAAGGTTATGAAACTGAGAAAGTTGGCACAGCAGGTTGCTAATTGCCGCCAGTGTCTTGA ACGGTCAACAGTCCTCATCAACCAAGCTGAGCATATCCTGAAAGAAAATGACCAGGCACGGTTTCTACAG TCTGCAAAAAATATTGCTGAGAGGGTCGCTATGGCAACTGCATCTTCTCAAGTTCTGATTCCAGACATCA ATTTTAATGATGCCTTTGAAAACTTTGCTTTAGATTTTTCCAGAGAAAAGAAACTGCTAGAGGGGTTAGA TTATTTAACAGCCCCAAACCCACCATCTATCCGAGAAGAACTCTGTACTGCCTCCCATGACACCATTACA GTCCACTGGATCTCGGATGATGAGTTCAGCATCAGCTCCTATGAGCTTCAGTACACCATATTCACTGGCC AGGCTAACTTCATCAGTAAGTCATGGTGTAGTTGGGGCCTGTGGCCAGAGATAAGGAAATGTAAGGAAGC AGTAAGCTGCTCAAGATTGGCCGGGGCGCCACGAGGCCTGTATAATTCAGTAGACAGCTGGATGATTGTT CCCAACATTAAACAGAACCATTACACAGTGCATGGACTCCAGAGCGGGACTCGCTACATCTTCATCGTTA AAGCCATAAACCAAGCCGGCAGCCGGAACAGTGAACCTACCCGACTAAAAACAAACAGCCAACCCTTTAA ATTGGATCCCAAAATGACTCACAAGAAGTTGAAGATCTCCAATGATGGATTGCAGATGGAGAAGGATGAA AGCTCTCTAAAGAAGAGCCACACCCCAGAGAGGTTTAGTGGCACAGGGTGCTATGGGGCAGCAGGAAATA TATTCATTGACAGTGGCTGCCACTATTGGGAGGTGGTCATGGGTTCCTCAACATGGTATGCAATTGGCAT TGCCTACAAATCAGCTCCAAAGAATGAATGGATTGGCAAGAATGCCTCCTCATGGGTCTTCTCTCGCTGC AATAGTAACTTCGTGGTGAGACACAACAACAAGGAAATGCTGGTGGATGTGCCCCCACACCTGAAGCGTC TGGGTGTCCTCCTGGATTATGACAACAATATGCTGTCTTTCTATGACCCAGCTAACTCTCTCCATCTTCA TACTTTTGATGTGACCTTCATTCTTCCAGTTTGTCCAACATTTACAATCTGGAACAAATCCCTAATGATC CTGTCTGGCTTGCCTGCCCCAGATTTTATTGATTACCCTGAGCGGCAGGAATGCAACTGCAGGCCTCAAG AATCCCCTTATGTTTCTGGGATGAAAACCTGTCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229356 representing NM_012216
Red=Cloning site Green=Tags(s) MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEP ITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQ FCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSD DQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEA KLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQ SAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTIT VHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIV PNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDE SSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRC NSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMI LSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012216 |
| ORF Size | 2205 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012216.4 |
| RefSeq Size | 7345 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 11043 |
| UniProt ID | Q9UJV3 |
| Domains | zf-B_box, RING, BBC, SPRY, FN3 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229356 | MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 5,384.00 |
|
| RC229356L3 | Lenti-ORF clone of MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 7,700.00 |
|
| RC229356L4 | Lenti-ORF clone of MID2 (mGFP-tagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 7,700.00 |
|
| SC115493 | MID2 (untagged)-Human midline 2 (MID2), transcript variant 1 |
CNY 5,248.00 |
|
| SC327991 | MID2 (untagged)-Human midline 2 (MID2) transcript variant 1 |
CNY 14,060.00 |
