United States
Germany
Japan
United Kingdom
China
MICAL1 (NM_001159291) Human Untagged Clone
CAT＃: SC327685
MICAL1 (untagged)-Human microtubule associated monoxygenase calponin and LIM domain containing 1 (MICAL1) transcript variant 2
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MICAL; MICAL-1; NICAL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327685 representing NM_001159291.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTTCACCTACCTCCACCAACCCAGCGCATGCCCACTTTGAGAGCTTCCTGCAGGCCCAGCTGTGC CAGGACGTGCTGAGCAGCTTCCAGGAGCTGTGTGGGGCCCTGGGGCTGGAACCCGGTGGGGGGCTGCCC CAGTACCACAAGATCAAGGACCAGCTCAACTACTGGAGCGCCAAGTCACTGTGGACCAAGCTGGACAAG CGAGCAGGCCAGCCTGTCTACCAGCAGGGCCGGGCCTGCACCAGCACCAAGTGCCTGGTGGTGGGTGCT GGACCTTGCGGGCTGCGGGTCGCTGTGGAGCTGGCGCTGCTGGGGGCCCGAGTGGTGCTGGTGGAAAAG CGCACCAAGTTCTCTCGCCACAACGTGCTCCACCTCTGGCCCTTCACCATCCACGACCTGCGGGCACTC GGTGCTAAGAAGTTCTACGGGCGCTTCTGCACCGGCACCCTGGACCACATCAGCATCAGGCAGCTCCAG CTGCTTCTGCTGAAGGTAGCATTGCTGCTGGGGGTGGAAATTCACTGGGGTGTCACTTTCACTGGCCTC CAGCCCCCTCCTAGGAAGGGGAGTGGCTGGCGTGCCCAGCTCCAACCCAACCCCCCTGCCCAGCTGGCC AACTATGAATTTGACGTCCTTATCTCGGCTGCAGGAGGTAAATTCGTCCCTGAAGGCTTCAAAGTTCGA GAAATGCGAGGCAAACTGGCCATTGGCATCACAGCCAACTTTGTGAATGGACGCACCGTGGAGGAGACA CAGGTGCCGGAGATCAGTGGTGTAGCCAGGATCTACAACCAGAGCTTCTTCCAGAGCCTTCTCAAAGCC ACAGGCATTGATCTGGAGAACATTGTGTACTACAAGGACGACACCCACTACTTTGTGATGACAGCCAAG AAGCAGTGCCTGCTGCGGCTGGGGGTGCTGCGCCAGCCCTTCTGGCCCCTGGGCACTGGAGTGGCACGG GGCTTCCTGGCAGCCTTTGATGCAGCCTGGATGGTGAAGCGGTGGGCAGAGGGCGCTGAGTCCCTAGAG GTGTTGGCTGAGCGTGAGAGCCTGTACCAGCTTCTGTCACAGACATCCCCAGAAAACATGCATCGCAAT GTGGCCCAGTATGGGCTGGACCCAGCCACCCGCTACCCCAACCTGAACCTCCGGGCAGTGACCCCCAAT CAGGTACGAGACCTGTATGATGTGCTAGCCAAGGAGCCTGTGCAGAGGAACAACGACAAGACAGATACA GGGATGCCAGCCACCGGGTCGGCAGGCACCCAGGAGGAGCTGCTACGCTGGTGCCAGGAGCAGACAGCT GGGTACCCGGGAGTCCACGTCTCCGATTTGTCTTCCTCCTGGGCTGATGGGCTAGCTCTGTGTGCCCTG GTGTACCGGCTGCAGCCTGGCCTGCTGGAACCCTCAGAGCTGCAGGGGCTGGGAGCTCTGGAAGCAACT GCTTGGGCACTAAAGGTGGCAGAGAATGAGCTGGGCATCACACCGGTGGTGTCTGCACAGGCCGTGGTA GCAGGGAGTGACCCACTGGGCCTCATTGCCTACCTCAGCCACTTCCACAGTGCCTTCAAGAGCATGGCC CACAGCCCAGGCCCTGTCAGCCAGGCCTCCCCAGGGACCTCCAGTGCTGTATTATTCCTTAGTAAACTT CAGAGGACCCTGCAGCGATCCCGGGCCAAGGAAAATGCAGAGGATGCTGGTGGCAAGAAGCTGCGCTTG GAGATGGAGGCCGAGACCCCAAGTACTGAGGTGCCACCTGACCCAGAGCCTGGTGTACCCCTGACACCC CCATCCCAACACCAGGAGGCCGGTGCTGGGGACCTGTGTGCACTTTGTGGGGAACACCTCTATGTCCTG GAACGCCTCTGTGTCAACGGCCATTTCTTCCACCGGAGCTGCTTCCGCTGCCATACCTGTGAGGCCACA CTGTGGCCAGGTGGCTACGAGCAGCACCCAGGAGATGGACATTTCTACTGCCTCCAGCACCTGCCCCAG ACAGACCACAAAGCGGAAGGCAGCGATAGAGGCCCTGAGAGTCCGGAGCTCCCCACACCAAGTGAGAAT AGCATGCCACCAGGCCTCTCAACTCCCACAGCCTCGCAGGAGGGGGCCGGTCCTGTTCCAGATCCCAGC CAGCCCACCCGTCGGCAGATCCGCCTCTCCAGCCCGGAGCGCCAGCGGTTGTCCTCCCTTAACCTTACC CCTGACCCGGAAATGGAGCCTCCACCCAAGCCTCCCCGCAGCTGCTCCGCCTTGGCCCGCCACGCCCTG GAGAGCAGCTTTGTGGGCTGGGGCCTGCCAGTCCAGAGCCCTCAAGCTCTTGTGGCCATGGAGAAGGAG GAAAAAGAGAGTCCCTTCTCCAGTGAAGAGGAAGAAGAAGATGTGCCTTTGGACTCAGATGTGGAACAG GCCCTGCAGACCTTTGCCAAGACCTCAGGCACCATGAATAACTACCCAACATGGCGTCGGACTCTGCTG CGCCGTGCGAAGGAGGAGGAGATGAAGAGGTTCTGCAAGGCCCAGACCATCCAACGGCGACTAAATGAG ATTGAGGCTGCCTTGAGGGAGCTAGAGGCCGAGGGCGTGAAGCTGGAGCTGGCCTTGAGGCGCCAGAGC AGTTCCCCAGAACAGCAAAAGAAACTATGGGTAGGACAGCTGCTACAGCTCGTTGACAAGAAAAACAGC CTGGTGGCTGAGGAGGCCGAGCTCATGATCACGGTGCAGGAATTGAATCTGGAGGAGAAACAGTGGCAG CTGGACCAGGAGCTACGAGGCTACATGAACCGGGAAGAAAACCTAAAGACAGCTGCTGATCGGCAGGCT GAGGACCAGGTCCTGAGGAAGCTGGTGGATTTGGTCAACCAGAGAGATGCCCTCATCCGCTTCCAGGAG GAGCGCAGGCTCAGCGAGCTGGCCTTGGGGACAGGGGCCCAGGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001159291 |
| Insert Size | 2946 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001159291.1 |
| RefSeq Size | 3403 bp |
| RefSeq ORF | 2946 bp |
| Locus ID | 64780 |
| UniProt ID | Q8TDZ2 |
| MW | 108.6 kDa |
| Gene Summary | This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (2) contains an alternate 5' exon and thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, and it lacks an alternate in-frame exon in the central coding region, compared to variant 3. The encoded isoform (2, also known as MICAL-1b) is shorter at the N-terminus, compared to isoform 3. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229050 | MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 10,776.00 |
|
| RC229050L1 | Lenti-ORF clone of MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 13,176.00 |
|
| RC229050L2 | Lenti-ORF clone of MICAL1 (mGFP-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 5,890.00 |
|
| RC229050L3 | Lenti-ORF clone of MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 5,890.00 |
|
| RC229050L4 | Lenti-ORF clone of MICAL1 (mGFP-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 5,890.00 |
|
| RG229050 | MICAL1 (tGFP-tagged) - Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 4,370.00 |
