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MICAL1 (NM_001159291) Human Tagged ORF Clone
CAT＃: RC229050
- TrueORF®
MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2
ORF Plasmid: tGFP
"NM_001159291" in other vectors (6)
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CNY 10,776.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MICAL; MICAL-1; NICAL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229050 representing NM_001159291
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCACCTACCTCCACCAACCCAGCGCATGCCCACTTTGAGAGCTTCCTGCAGGCCCAGCTGTGCC AGGACGTGCTGAGCAGCTTCCAGGAGCTGTGTGGGGCCCTGGGGCTGGAACCCGGTGGGGGGCTGCCCCA GTACCACAAGATCAAGGACCAGCTCAACTACTGGAGCGCCAAGTCACTGTGGACCAAGCTGGACAAGCGA GCAGGCCAGCCTGTCTACCAGCAGGGCCGGGCCTGCACCAGCACCAAGTGCCTGGTGGTGGGTGCTGGAC CTTGCGGGCTGCGGGTCGCTGTGGAGCTGGCGCTGCTGGGGGCCCGAGTGGTGCTGGTGGAAAAGCGCAC CAAGTTCTCTCGCCACAACGTGCTCCACCTCTGGCCCTTCACCATCCACGACCTGCGGGCACTCGGTGCT AAGAAGTTCTACGGGCGCTTCTGCACCGGCACCCTGGACCACATCAGCATCAGGCAGCTCCAGCTGCTTC TGCTGAAGGTAGCATTGCTGCTGGGGGTGGAAATTCACTGGGGTGTCACTTTCACTGGCCTCCAGCCCCC TCCTAGGAAGGGGAGTGGCTGGCGTGCCCAGCTCCAACCCAACCCCCCTGCCCAGCTGGCCAACTATGAA TTTGACGTCCTTATCTCGGCTGCAGGAGGTAAATTCGTCCCTGAAGGCTTCAAAGTTCGAGAAATGCGAG GCAAACTGGCCATTGGCATCACAGCCAACTTTGTGAATGGACGCACCGTGGAGGAGACACAGGTGCCGGA GATCAGTGGTGTAGCCAGGATCTACAACCAGAGCTTCTTCCAGAGCCTTCTCAAAGCCACAGGCATTGAT CTGGAGAACATTGTGTACTACAAGGACGACACCCACTACTTTGTGATGACAGCCAAGAAGCAGTGCCTGC TGCGGCTGGGGGTGCTGCGCCAGCCCTTCTGGCCCCTGGGCACTGGAGTGGCACGGGGCTTCCTGGCAGC CTTTGATGCAGCCTGGATGGTGAAGCGGTGGGCAGAGGGCGCTGAGTCCCTAGAGGTGTTGGCTGAGCGT GAGAGCCTGTACCAGCTTCTGTCACAGACATCCCCAGAAAACATGCATCGCAATGTGGCCCAGTATGGGC TGGACCCAGCCACCCGCTACCCCAACCTGAACCTCCGGGCAGTGACCCCCAATCAGGTACGAGACCTGTA TGATGTGCTAGCCAAGGAGCCTGTGCAGAGGAACAACGACAAGACAGATACAGGGATGCCAGCCACCGGG TCGGCAGGCACCCAGGAGGAGCTGCTACGCTGGTGCCAGGAGCAGACAGCTGGGTACCCGGGAGTCCACG TCTCCGATTTGTCTTCCTCCTGGGCTGATGGGCTAGCTCTGTGTGCCCTGGTGTACCGGCTGCAGCCTGG CCTGCTGGAACCCTCAGAGCTGCAGGGGCTGGGAGCTCTGGAAGCAACTGCTTGGGCACTAAAGGTGGCA GAGAATGAGCTGGGCATCACACCGGTGGTGTCTGCACAGGCCGTGGTAGCAGGGAGTGACCCACTGGGCC TCATTGCCTACCTCAGCCACTTCCACAGTGCCTTCAAGAGCATGGCCCACAGCCCAGGCCCTGTCAGCCA GGCCTCCCCAGGGACCTCCAGTGCTGTATTATTCCTTAGTAAACTTCAGAGGACCCTGCAGCGATCCCGG GCCAAGGAAAATGCAGAGGATGCTGGTGGCAAGAAGCTGCGCTTGGAGATGGAGGCCGAGACCCCAAGTA CTGAGGTGCCACCTGACCCAGAGCCTGGTGTACCCCTGACACCCCCATCCCAACACCAGGAGGCCGGTGC TGGGGACCTGTGTGCACTTTGTGGGGAACACCTCTATGTCCTGGAACGCCTCTGTGTCAACGGCCATTTC TTCCACCGGAGCTGCTTCCGCTGCCATACCTGTGAGGCCACACTGTGGCCAGGTGGCTACGAGCAGCACC CAGGAGATGGACATTTCTACTGCCTCCAGCACCTGCCCCAGACAGACCACAAAGCGGAAGGCAGCGATAG AGGCCCTGAGAGTCCGGAGCTCCCCACACCAAGTGAGAATAGCATGCCACCAGGCCTCTCAACTCCCACA GCCTCGCAGGAGGGGGCCGGTCCTGTTCCAGATCCCAGCCAGCCCACCCGTCGGCAGATCCGCCTCTCCA GCCCGGAGCGCCAGCGGTTGTCCTCCCTTAACCTTACCCCTGACCCGGAAATGGAGCCTCCACCCAAGCC TCCCCGCAGCTGCTCCGCCTTGGCCCGCCACGCCCTGGAGAGCAGCTTTGTGGGCTGGGGCCTGCCAGTC CAGAGCCCTCAAGCTCTTGTGGCCATGGAGAAGGAGGAAAAAGAGAGTCCCTTCTCCAGTGAAGAGGAAG AAGAAGATGTGCCTTTGGACTCAGATGTGGAACAGGCCCTGCAGACCTTTGCCAAGACCTCAGGCACCAT GAATAACTACCCAACATGGCGTCGGACTCTGCTGCGCCGTGCGAAGGAGGAGGAGATGAAGAGGTTCTGC AAGGCCCAGACCATCCAACGGCGACTAAATGAGATTGAGGCTGCCTTGAGGGAGCTAGAGGCCGAGGGCG TGAAGCTGGAGCTGGCCTTGAGGCGCCAGAGCAGTTCCCCAGAACAGCAAAAGAAACTATGGGTAGGACA GCTGCTACAGCTCGTTGACAAGAAAAACAGCCTGGTGGCTGAGGAGGCCGAGCTCATGATCACGGTGCAG GAATTGAATCTGGAGGAGAAACAGTGGCAGCTGGACCAGGAGCTACGAGGCTACATGAACCGGGAAGAAA ACCTAAAGACAGCTGCTGATCGGCAGGCTGAGGACCAGGTCCTGAGGAAGCTGGTGGATTTGGTCAACCA GAGAGATGCCCTCATCCGCTTCCAGGAGGAGCGCAGGCTCAGCGAGCTGGCCTTGGGGACAGGGGCCCAG GGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229050 representing NM_001159291
Red=Cloning site Green=Tags(s) MASPTSTNPAHAHFESFLQAQLCQDVLSSFQELCGALGLEPGGGLPQYHKIKDQLNYWSAKSLWTKLDKR AGQPVYQQGRACTSTKCLVVGAGPCGLRVAVELALLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGA KKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVTFTGLQPPPRKGSGWRAQLQPNPPAQLANYE FDVLISAAGGKFVPEGFKVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGID LENIVYYKDDTHYFVMTAKKQCLLRLGVLRQPFWPLGTGVARGFLAAFDAAWMVKRWAEGAESLEVLAER ESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVRDLYDVLAKEPVQRNNDKTDTGMPATG SAGTQEELLRWCQEQTAGYPGVHVSDLSSSWADGLALCALVYRLQPGLLEPSELQGLGALEATAWALKVA ENELGITPVVSAQAVVAGSDPLGLIAYLSHFHSAFKSMAHSPGPVSQASPGTSSAVLFLSKLQRTLQRSR AKENAEDAGGKKLRLEMEAETPSTEVPPDPEPGVPLTPPSQHQEAGAGDLCALCGEHLYVLERLCVNGHF FHRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESPELPTPSENSMPPGLSTPT ASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNLTPDPEMEPPPKPPRSCSALARHALESSFVGWGLPV QSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQALQTFAKTSGTMNNYPTWRRTLLRRAKEEEMKRFC KAQTIQRRLNEIEAALRELEAEGVKLELALRRQSSSPEQQKKLWVGQLLQLVDKKNSLVAEEAELMITVQ ELNLEEKQWQLDQELRGYMNREENLKTAADRQAEDQVLRKLVDLVNQRDALIRFQEERRLSELALGTGAQ G TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159291 |
| ORF Size | 2943 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159291.1, NP_001152763.1 |
| RefSeq ORF | 2946 bp |
| Locus ID | 64780 |
| UniProt ID | Q8TDZ2 |
| MW | 108.4 kDa |
| Gene Summary | This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229050L1 | Lenti-ORF clone of MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 13,176.00 |
|
| RC229050L2 | Lenti-ORF clone of MICAL1 (mGFP-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 5,890.00 |
|
| RC229050L3 | Lenti-ORF clone of MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 5,890.00 |
|
| RC229050L4 | Lenti-ORF clone of MICAL1 (mGFP-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 5,890.00 |
|
| RG229050 | MICAL1 (tGFP-tagged) - Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 2 |
CNY 4,370.00 |
|
| SC327685 | MICAL1 (untagged)-Human microtubule associated monoxygenase calponin and LIM domain containing 1 (MICAL1) transcript variant 2 |
CNY 7,220.00 |
