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Rubicon (RUBCN) (NM_001145642) Human Untagged Clone
CAT＃: SC326632
KIAA0226 (untagged)-Human KIAA0226 (KIAA0226), transcript variant 1, mRNA
CN¥ 17,860.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA0226; RUBICON; SCAR15 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001145642, the custom clone sequence may differ by one or more nucleotides
ATGCAGAGCATCCTCTATCACGGGCTTATCCGTGACCAGGCGTGCCGCCGCCAGACGGAT TACTGGCAGTTCGTGAAAGACATCCGGTGGCTCAGTCCCCACTCAGCCCTTCACGTGGAG AAGTTCATCAGCGTGCACGAGAACGACCAGAGCAGTGCTGATGGTGCCAGTGAACGTGCT GTTGCCGAGCTGTGGCTGCAGCACAGCCTGCAGTACCACTGCCTCTCAGCCCAGCTCCGG CCCCTGCTCGGGGATAGACAGTATATCAGAAAATTCTACACAGATGCTGCCTTCCTGCTA AGTGACGCTCATGTCACGGCCATGCTGCAGTGCCTGGAAGCAGTGGAACAGAACAACCCC CGCCTCCTGGCTCAGATCGATGCGTCCATGTTTGCCAGAAAGCACGAGAGCCCGCTCCTG GTGACAAAGAGCCAGAGCCTGACAGCCCTGCCCAGTTCCACATACACCCCTCCAAACAGC TATGCTCAGCATTCCTACTTTGGGTCCTTCTCTAGCCTCCACCAATCCGTGCCCAACAAT GGCTCAGAGAGAAGATCTACTTCCTTTCCACTCTCTGGCCCTCCCCGGAAACCTCAAGAA AGCAGAGGGCACGTCTCACCAGCAGAGGATCAAACCATCCAAGCCCCCCCAGTTTCAGTC TCTGCACTAGCCAGGGATTCCCCTTTGACCCCAAATGAAATGAGCTCCAGTACTCTGACC AGCCCCATAGAGGCATCCTGGGTCAGCAGCCAGAATGATTCCCCAGGTGATGCCAGTGAG GGGCCTGAGTACCTGGCCATTGGCAACTTGGACCCCCGAGGCCGGACTGCCAGCTGTCAG AGTCACAGCAGCAATGCCGAGAGCAGCAGTTCCAATTTGTTCTCCTCCAGCAGCTCCCAG AAGCCAGATTCTGCTGCCTCTTCCTTAGGGGACCAGGAAGGAGGTGGGGAGAGCCAGCTG TCCAGTGTCCTCCGCAGGTCCAGCTTCTCAGAGGGGCAGACACTCACTGTCACCAGTGGG GCAAAGAAAAGCCACATTCGCTCCCATTCGGATACCAGCATTGCCTCCAGGGGAGCTCCA GGAGGCCCCAGGAATATCACCATTATAGTTGAAGATCCCATTGCAGAATCCTGCAATGAT AAGGCGAAGTTGAGAGGCCCTTTGCCCTACTCTGGTCAAAGCAGTGAAGTCAGCACACCC AGCTCTCTGTACATGGAATATGAAGGTGGTCGGTACCTGTGCTCAGGGGAAGGCATGTTC CGAAGACCATCAGAAGGACAGTCCCTCATCAGCTACCTCTCTGAGCAAGACTTCGGCAGC TGTGCCGACCTGGAAAAGGAGAATGCCCACTTCAGCATCTCAGAGTCCTTAATTGCTGCC ATCGAGCTAATGAAGTGCAACATGATGAGCCAGTGCCTAGAGGAGGAGGAAGTGGAAGAG GAAGACAGTGATAGAGAGATCCAGGAGCTGAAGCAGAAGATCCGCCTTCGGCGCCAGCAA ATCCGCACCAAGAACCTGCTCCCCATGTACCAGGAGGCTGAGCACGGAAGCTTTCGGGTC ACCTCCAGCAGCTCCCAGTTCAGCTCACGTGATTCGGCACAGCTCTCTGACTCTGGCTCT GCTGATGAGGTTGATGAATTTGAAATCCAAGATGCTGACATCAGAAGGAACACAGCCTCA AGCAGCAAATCCTTCGTTTCCTCCCAGTCCTTCTCCCACTGCTTCCTGCACTCCACGTCT GCTGAGGCGGTGGCCATGGGGCTCCTGAAGCAGTTTGAGGGGATGCAGCTTCCAGCCGCC TCGGAGCTGGAGTGGCTTGTCCCGGAGCATGATGCCCCTCAGAAGCTCCTGCCCATTCCT GACTCACTGCCCATCTCACCGGATGACGGGCAGCACGCTGACATCTACAAGCTGCGGATT CGTGTTCGTGGCAACTTGGAGTGGGCCCCGCCCCGGCCTCAGATAATTTTTAATGTTCAT CCAGCCCCAACGAGGAAAATTGCCGTGGCCAAGCAGAATTACCGCTGTGCAGGATGTGGC ATCCGGACTGACCCTGATTACATCAAGCGACTGCGGTACTGTGAGTACCTGGGCAAGTAC TTCTGCCAGTGCTGCCACGAGAATGCCCAGATGGCCATCCCCAGCCGGGTTCTGCGCAAG TGGGACTTCAGCAAGTACTACGTCAGCAACTTCTCCAAGGACCTGCTCATTAAGATCTGG AATGATCCTCTCTTCAACGTGCAGGACATAAACAGTGCCCTCTATAGGAAGGTCAAGCTG CTCAATCAAGTCCGGCTGCTGCGGGTCCAGCTGTGTCACATGAAGAACATGTTCAAGACT TGCCGACTGGCCAAGGAGCTTCTGGATTCCTTTGACACAGTCCCAGGCCACCTGACAGAG GACCTCCACCTGTACTCACTGAATGACCTGACTGCGACCAGGAAGGGGGAGCTGGGGCCC CGGCTTGCTGAGCTCACCAGGGCAGGGGCTACCCATGTGGAGAGATGCATGCTCTGCCAA GCCAAAGGCTTCATCTGTGAGTTCTGTCAGAATGAGGATGACATCATCTTTCCCTTTGAG CTCCATAAGTGCCGGACCTGTGAAGAGTGTAAAGCGTGTTACCATAAAGCCTGCTTCAAG TCTGGAAGCTGTCCGCGCTGCGAGCGGCTGCAGGCCCGGCGGGAGGCACTGGCCAGGCAG AGCCTGGAGTCTTACCTGTCAGACTACGAGGAGGAGCCCGCGGAAGCGCTGGCCCTGGAA GCCGCCGTCCTGGAGGCCACC |
| Restriction Sites | Please inquire |
| ACCN | NM_001145642 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145642.2, NP_001139114.1 |
| RefSeq Size | 6903 bp |
| RefSeq ORF | 2784 bp |
| Locus ID | 9711 |
| UniProt ID | Q92622 |
| Gene Summary | The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (1) has an alternate 5' exon, which results in translation initiation from a downstream start codon, compared to variant 2. The resulting isoform (1) has a shorter N-terminus, compared to isoform 2. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227149 | KIAA0226 (Myc-DDK-tagged)-Human KIAA0226 (KIAA0226), transcript variant 1 |
CN¥ 7,130.00 |
|
| RC227149L3 | Lenti-ORF clone of KIAA0226 (Myc-DDK-tagged)-Human KIAA0226 (KIAA0226), transcript variant 1 |
CN¥ 9,030.00 |
|
| RC227149L4 | Lenti-ORF clone of KIAA0226 (mGFP-tagged)-Human KIAA0226 (KIAA0226), transcript variant 1 |
CN¥ 9,030.00 |
|
| RG227149 | KIAA0226 (tGFP-tagged) - Human KIAA0226 (KIAA0226), transcript variant 1 |
CN¥ 7,890.00 |
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