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SUPT5H (NM_001130825) Human Untagged Clone
CAT＃: SC326334
SUPT5H (untagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 4
CNY 7,552.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SPT5; SPT5H; Tat-CT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326334 representing NM_001130825.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGACAGCGAGGACAGCAACTTTTCCGAGGAGGAGGACAGCGAGCGCAGCAGTGACGGCGAGGAG GCCGAGGTAGACGAAGAGCGGCGGAGTGCAGCGGGCAGTGAGAAAGAAGAAGAGCCTGAGGACGAAGAG GAGGAGGAAGAGGAGGAGGAATACGATGAGGAAGAGGAGGAAGAAGATGATGACCGACCCCCCAAGAAA CCCCGCCATGGAGGCTTCATTCTGGACGAGGCTGATGTTGACGATGAGTATGAGGACGAGGACCAGTGG GAGGATGGAGCAGAGGACATTCTAGAGAAAGCCTCCAATATCGATAATGTTGTCCTGGATGAAGATCGT TCTGGGGCTCGCCGCCTGCAAAACCTCTGGAGGGACCAGCGAGAAGAAGAACTGGGCGAGTATTACATG AAGAAATACGCCAAGTCATCTGTGGGAGAGACGGTGTATGGAGGATCTGATGAGCTCTCAGACGACATC ACCCAGCAGCAGCTGCTCCCAGGAGTCAAGGATCCCAATCTGTGGACTGTCAAATGTAAGATTGGGGAG GAACGGGCCACGGCCATTTCCTTGATGCGCAAGTTCATTGCCTACCAGTTCACAGACACGCCCCTGCAG ATCAAGTCAGTAGTGGCACCAGAGCATGTGAAGGGCTACATCTACGTGGAGGCCTACAAGCAGACCCAC GTGAAGCAGGCCATTGAGGGGGTGGGCAACCTGCGGCTTGGCTACTGGAACCAGCAGATGGTGCCCATC AAGGAGATGACAGACGTGCTCAAAGTGGTGAAGGAGGTGGCCAACCTGAAACCAAAGTCCTGGGTCCGC CTCAAGCGGGGCATCTACAAGGATGACATTGCTCAGGTGGACTACGTGGAGCCCAGCCAGAACACCATC TCCCTGAAGATGATCCCACGCATCGACTACGATCGCATCAAGGCCCGCATGAGCTTGAAAGACTGGTTT GCCAAAAGGAAGAAGTTTAAGCGGCCTCCACAGAGGCTGTTTGATGCTGAGAAGATCAGGTCCCTGGGG GGTGATGTTGCCTCTGATGGTGACTTCCTCATCTTTGAGGGGAACCGTTACAGCCGGAAGGGCTTTCTG TTCAAGAGCTTCGCCATGTCTGCTGTGATCACGGAGGGTGTGAAGCCAACACTCTCTGAGCTGGAAAAG TTTGAGGACCAGCCAGAGGGCATTGACCTGGAGGTGGTGACTGAGAGCACAGGGAAGGAGCGGGAGCAC AACTTCCAACCTGGGGACAACGTGGAGGTCTGTGAGGGTGAGCTCATCAACCTGCAGGGCAAGATCCTC AGCGTGGATGGCAACAAGATCACCATCATGCCCAAGCATGAGGACCTCAAGGACATGTTGGAGTTCCCA GCCCAGGAACTTAGAAAATACTTCAAGATGGGGGACCACGTGAAGGTGATTGCTGGCCGATTCGAGGGC GACACAGGCCTCATTGTGCGGGTGGAGGAGAATTTCGTTATCCTGTTCTCTGACCTCACCATGCATGAG CTGAAGGTGCTCCCCCGGGACCTGCAGCTCTGCTCAGAGACAGCATCAGGTGTGGATGTTGGGGGCCAG CATGAATGGGGCGAGCTGGTGCAGCTGGATCCCCAGACTGTGGGTGTCATCGTGCGACTAGAACGGGAG ACCTTCCAGGTGCTGAACATGTACGGGAAGGTGGTGACTGTCAGACATCAGGCTGTGACCCGGAAGAAG GACAACCGCTTTGCTGTGGCCTTGGACTCAGAGCAGAACAACATCCATGTGAAAGACATCGTTAAGGTC ATTGATGGCCCCCACTCAGGCCGAGAAGGGGAGATTCGCCATCTCTTCCGAAGCTTCGCCTTCCTACAT TGCAAGAAACTGGTGGAGAACGGGGGCATGTTTGTCTGCAAGACCCGCCACCTGGTGCTGGCTGGGGGC TCAAAGCCCCGTGATGTGACCAACTTCACCGTGGGTGGCTTTGCGCCTATGAGTCCCCGGATCAGCAGC CCCATGCACCCCAGTGCTGGAGGTCAGCGTGGCGGCTTTGGTAGCCCAGGTGGCGGCAGTGGTGGCATG AGCAGGGGCCGGGGCCGGAGGGACAACGAACTCATCGGCCAGACCGTGCGCATCTCCCAGGGGCCCTAC AAAGGCTACATCGGTGTGGTGAAAGATGCCACAGAGTCCACGGCCCGTGTGGAGCTGCACTCCACCTGC CAGACCATCTCTGTGGACCGTCAGCGGCTCACCACGGTGGGCTCACGGCGCCCGGGCGGCATGACCTCG ACCTATGGGAGGACGCCCATGTATGGCTCCCAGACGCCCATGTATGGCTCTGGCTCCCGAACACCCATG TACGGCTCACAGACACCCCTCCAGGATGGTAGCCGCACCCCACACTACGGCTCACAGACGCCCCTGCAT GATGGCAGCCGCACTCCTGCCCAGAGTGGGGCCTGGGACCCCAACAACCCCAACACGCCGTCACGGGCT GAGGAAGAATATGAGTATGCTTTCGATGATGAGCCCACCCCGTCCCCGCAGGCCTATGGGGGAACCCCC AATCCCCAAACACCTGGCTACCCAGACCCCTCGTCCCCACAGGTCAACCCACAATACAACCCGCAGACG CCAGGGACGCCGGCCATGTACAACACAGACCAGTTCTCTCCCTATGCTGCCCCCTCCCCACAAGGTTCC TACCAGCCCAGCCCCAGCCCCCAGAGCTACCACCAGGTGGCGCCAAGCCCAGCAGGCTACCAGAATACC CACTCCCCAGCCAGCTACCACCCTACACCGTCGCCCATGGCCTATCAGGCTAGCCCCAGCCCGAGCCCC GTTGGCTACAGTCCTATGACACCTGGAGCTCCCTCCCCTGGTGGCTACAACCCACACACGCCAGGCTCA GGCATCGAGCAGAACTCCAGCGACTGGGTAACCACTGACATTCAGGTGAAGGTGCGGGACACCTACCTG GATACACAGGTGGTGGGACAGACAGGTGTCATCCGCAGTGTCACGGGGGGCATGTGCTCTGTGTACCTG AAGGACAGTGAGAAGGTTGTCAGCATTTCCAGTGAGCACCTGGAGCCTATCACCCCCACCAAGAACAAC AAGGTGAAAGTGATCCTGGGCGAGGATCGGGAAGCCACGGGCGTCCTACTGAGCATTGATGGTGAGGAT GGCATTGTCCGTATGGACCTTGATGAGCAGCTCAAGATCCTCAACCTCCGCTTCCTGGGGAAGCTCCTG GAAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001130825 |
| Insert Size | 3252 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130825.1 |
| RefSeq Size | 3722 bp |
| RefSeq ORF | 3252 bp |
| Locus ID | 6829 |
| UniProt ID | O00267 |
| Protein Families | Transcription Factors |
| MW | 120.5 kDa |
| Gene Summary | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 4 and 6 encode the same isoform (b). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226318 | SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 4 |
CNY 7,544.00 |
|
| RC226318L1 | Lenti ORF clone of Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 4, Myc-DDK-tagged |
CNY 9,944.00 |
|
| RC226318L2 | Lenti ORF clone of Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 4, mGFP tagged |
CNY 12,730.00 |
|
| RC226318L3 | Lenti ORF clone of Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 4, Myc-DDK-tagged |
CNY 12,730.00 |
|
| RC226318L4 | Lenti ORF clone of Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 4, mGFP tagged |
CNY 12,730.00 |
|
| RG226318 | SUPT5H (tGFP-tagged) - Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 4 |
CNY 11,880.00 |
