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RPA70 (RPA1) (NM_002945) Human Untagged Clone
CAT＃: SC324660
RPA1 (untagged)-Human replication protein A1, 70kDa (RPA1)
CNY 4,592.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSSB; MST075; REPA1; RF-A; RP-A; RPA70 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_002945.2
GGAGCTGTTGCGGGGTCCGCGGGGAAGTCTTGGCGGTGGAGCCATGGTCGGCCAACTGAG
CGAGGGGGCCATTGCGGCCATCATGCAGAAGGGGGATACAAACATAAAGCCCATCCTCCA AGTCATCAACATCCGTCCCATTACTACGGGGAATAGTCCGCCGCGTTATCGACTGCTCAT GAGTGATGGATTGAACACTCTATCCTCTTTCATGTTGGCGACACAGTTGAACCCTCTCGT GGAGGAAGAACAATTGTCCAGCAACTGTGTATGCCAGATTCACAGATTTATTGTGAACAC TCTGAAAGACGGAAGGAGAGTAGTTATCTTGATGGAATTAGAAGTTTTGAAGTCAGCTGA AGCAGTTGGAGTGAAGATTGGCAATCCAGTGCCCTATAATGAAGGACTCGGGCAGCCGCA AGTAGCTCCTCCAGCGCCAGCAGCCAGCCCAGCAGCAAGCAGCAGGCCCCAGCCGCAGAA TGGAAGCTCGGGAATGGGTTCTACTGTTTCTAAGGCTTATGGTGCTTCAAAGACATTTGG AAAAGCTGCAGGTCCCAGCCTGTCACACACTTCTGGGGGAACACAGTCCAAAGTGGTGCC CATTGCCAGCCTCACTCCTTACCAGTCCAAGTGGACCATTTGTGCTCGTGTTACCAACAA AAGTCAGATCCGTACCTGGAGCAACTCCCGAGGGGAAGGGAAGCTTTTCTCCCTAGAACT GGTTGACGAAAGTGGTGAAATCCGAGCTACAGCTTTCAATGAGCAAGTGGACAAGTTCTT TCCTCTTATTGAAGTGAACAAGGTGTATTATTTCTCGAAAGGCACCCTGAAGATTGCTAA CAAGCAGTTCACAGCTGTTAAAAATGACTACGAGATGACCTTCAATAACGAGACTTCCGT CATGCCCTGTGAGGACGACCATCATTTACCTACGGTTCAGTTTGATTTCACGGGGATTGA TGACCTCGAGAACAAGTCGAAAGACTCACTTGTAGACATCATCGGGATCTGCAAGAGCTA TGAAGACGCCACTAAAATCACAGTGAGGTCTAACAACAGAGAAGTTGCCAAGAGGAATAT CTACTTGATGGACACATCTGGGAAGGTGGTGACTGCTACACTGTGGGGGGAAGATGCTGA TAAATTTGATGGTTCTAGACAGCCCGTGTTGGCTATCAAAGGAGCCCGAGTCTCTGATTT CGGTGGACGGAGCCTCTCCGTGCTGTCTTCAAGCACTATCATTGCGAATCCTGACATCCC AGAGGCCTATAAGCTTCGTGGATGGTTTGACGCAGAAGGACAAGCCTTAGATGGTGTTTC CATCTCTGATCTAAAGAGCGGCGGAGTCGGAGGGAGTAACACCAACTGGAAAACCTTGTA TGAGGTCAAATCCGAGAACCTGGGCCAAGGCGACAAGCCGGACTACTTTAGTTCTGTGGC CACAGTGGTGTATCTTCGCAAAGAGAACTGCATGTACCAAGCCTGCCCGACTCAGGACTG CAATAAGAAAGTGATTGATCAACAGAATGGATTGTACCGCTGTGAGAAGTGCGACACCGA ATTTCCCAATTTCAAGTACCGCATGATCCTGTCAGTAAATATTGCAGATTTTCAAGAGAA TCAGTGGGTGACTTGTTTCCAGGAGTCTGCTGAAGCTATCCTTGGACAAAATGCTGCTTA TCTTGGGGAATTAAAAGACAAGAATGAACAGGCATTTGAAGAAGTTTTCCAGAATGCCAA CTTCCGATCTTTCATATTCAGAGTCAGGGTCAAAGTGGAGACCTACAACGACGAGTCTCG AATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGACTACAGAGAGTATGGCCGAAGGCT GGTCATGAGCATCAGGAGAAGTGCATTGATGTGAGAGGAGCAGTGCCAATCGGGCAGAAG TTTGCAAATAGGCAGAATGGAATCGATTTCCTCCCACCTCCGTGTGACGATCCCATGTTA GCTACACAGTGCAGAGGCTCTTGATGGTGGACTAAGCAATTTCCTCCCTTGTGCGCATCT CAGAACCCATCGGTAGGCAAAGGAAAATACGCTCAGGTGGTTGTGGTGTAGACTGTGTCA GGCCTACGGAGTCAGCCAGTGGCTAGCGCAAGACCAGTCACTCCCTCTGCCTTCAGGCTT CTGTCAATTTCATTATCATCAAGCAGGAATTATGTCGTAAGTCACTGACCCTAACTGCAG ACCATGAAGTAAATTATGTAACTAGGTTTTTGCTTCTCCAGTGGTGACCACCCCCCCCCC ATCCCCGCTCACAACTTGGGTTCTTCTCAGCGGGGCGAGCTGAGAAGCGGTCATGAGCAC CTGGGGATTTTAGTAAGTGTGTCTTCCTAGAATTCGAAGGCTCTCTCTTTCTAGAGGTGC TACATAGTTGGTAATGCTTGGAATGGCAATAGGGTAGAATGATTAATCAAAGGCATATCT TCTATATCTGAAGAGTATCCTTCCTTCAGGGTTTAATAGACTGAGTCAGATGGGTCTGAT ATTAATCAAAATTGTCTCTTCTGAGGACCGCTGATAAGCATTGACTTGCTGTCCCCTAAG GAAATCCGAGCGGCTACAAAGCGTTTCTTTACTTTTCACTTCAATTAATGCTGCGCTTCG CTTGGTGAGTGCGTACTTTTTCTACCTGTACACATTCCTGCATTCATGTATTTTGTTTTT TTTGACTAAAGCTATGTTACATGGAAAGGATTTTGAAGCCTTTTGTTTCCCTTGCTTTGT TTTAATAAACAGTATATTCTTTGGTTGTGAATCCTAAAAAAAAAAAAAAAAAAAAAAAAA AAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_002945 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002945.2, NP_002936.1 |
| RefSeq Size | 2824 bp |
| RefSeq ORF | 1851 bp |
| Locus ID | 6117 |
| UniProt ID | P27694 |
| Domains | tRNA_anti, Rep-A_N |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair |
| Gene Summary | This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202066 | RPA1 (Myc-DDK-tagged)-Human replication protein A1, 70kDa (RPA1) |
CNY 4,584.00 |
|
| RC202066L1 | Lenti ORF clone of Human replication protein A1, 70kDa (RPA1), Myc-DDK-tagged |
CNY 6,984.00 |
|
| RC202066L2 | Lenti ORF clone of Human replication protein A1, 70kDa (RPA1), mGFP tagged |
CNY 6,460.00 |
|
| RC202066L3 | Lenti ORF clone of Human replication protein A1, 70kDa (RPA1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202066L4 | Lenti ORF clone of Human replication protein A1, 70kDa (RPA1), mGFP tagged |
CNY 6,984.00 |
|
| RG202066 | RPA1 (tGFP-tagged) - Human replication protein A1, 70kDa (RPA1) |
CNY 6,184.00 |
|
| SC118289 | RPA1 (untagged)-Human replication protein A1, 70kDa (RPA1) |
CNY 4,592.00 |
