United States
Germany
Japan
United Kingdom
China
RPA70 (RPA1) (NM_002945) Human Untagged Clone
CAT＃: SC118289
RPA1 (untagged)-Human replication protein A1, 70kDa (RPA1)
CNY 4,592.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSSB; MST075; REPA1; RF-A; RP-A; RPA70 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118289 sequence for NM_002945 edited (data generated by NextGen Sequencing)
ATGGTCGGCCAGCTGAGCGAGGGGGCCATTGCGGCCATCATGCAGAAGGGGGATACAAAC ATAAAGCCCATCCTCCAAGTCATCAACATCCGTCCCATTACTACGGGGAATAGTCCGCCG CGTTATCGACTGCTCATGAGTGATGGATTGAACACTCTATCCTCTTTCATGTTGGCGACA CAGTTGAACCCTCTCGTGGAGGAAGAACAATTGTCCAGCAACTGTGTATGCCAGATTCAC AGATTTATTGTGAACACTCTGAAAGACGGAAGGAGAGTAGTTATCTTGATGGAATTAGAA GTTTTGAAGTCAGCTGAAGCAGTTGGAGTGAAGATTGGCAATCCAGTGCCCTATAATGAA GGACTCGGGCAGCCGCAAGTAGCTCCTCCAGCGCCAGCAGCCAGCCCAGCAGCAAGCAGC AGGCCCCAGCCGCAGAATGGAAGCTCGGGAATGGGTTCTACTGTTTCTAAGGCTTATGGT GCTTCAAAGACATTTGGAAAAGCTGCAGGTCCCAGCCTGTCACACACTTCTGGGGGAACA CAGTCCAAAGTGGTGCCCATTGCCAGCCTCACTCCTTACCAGTCCAAGTGGACCATTTGT GCTCGTGTTACCAACAAAAGTCAGATCCGTACCTGGAGCAACTCCCGAGGGGAAGGGAAG CTTTTCTCCCTAGAACTGGTTGACGAAAGTGGTGAAATCCGAGCTACAGCTTTCAATGAG CAAGTGGACAAGTTCTTTCCTCTTATTGAAGTGAACAAGGTGTATTATTTCTCGAAAGGC ACCCTGAAGATTGCTAACAAGCAGTTCACAGCTGTTAAAAATGACTACGAGATGACCTTC AATAACGAGACTTCCGTCATGCCCTGTGAGGACGACCATCATTTACCTACGGTTCAGTTT GATTTCACGGGGATTGATGACCTCGAGAACAAGTCGAAAGACTCACTTGTAGACATCATC GGGATCTGCAAGAGCTATGAAGACGCCACTAAAATCACAGTGAGGTCTAACAACAGAGAA GTTGCCAAGAGGAATATCTACTTGATGGACACATCCGGGAAGGTGGTGACTGCTACACTG TGGGGGGAAGATGCTGATAAATTTGATGGTTCTAGACAGCCCGTGTTGGCTATCAAAGGA GCCCGAGTCTCTGATTTCGGTGGACGGAGCCTCTCCGTGCTGTCTTCAAGCACTATCATT GCGAATCCTGACATCCCAGAGGCCTATAAGCTTCGTGGATGGTTTGACGCAGAAGGACAA GCCTTAGATGGTGTTTCCATCTCTGATCTAAAGAGCGGCGGAGTCGGAGGGAGTAACACC AACTGGAAAACCTTGTATGAGGTCAAATCCGAGAACCTGGGCCAAGGCGACAAGCCGGAC TACTTTAGTTCTGTGGCCACAGTGGTGTATCTTCGCAAAGAGAACTGCATGTACCAAGCC TGCCCGACTCAGGACTGCAATAAGAAAGTGATTGATCAACAGAATGGATTGTACCGCTGT GAGAAGTGCGACACCGAATTTCCCAATTTCAAGTACCGCATGATCCTGTCAGTAAATATT GCAGATTTTCAAGAGAATCAGTGGGTGACTTGTTTCCAGGAGTCTGCTGAAGCTATCCTT GGACAAAATGCTGCTTATCTTGGGGAATTAAAAGACAAGAATGAACAGGCATTTGAAGAA GTTTTCCAGAATGCCAACTTCCGATCTTTCATATTCAGAGTCAGGGTCAAAGTGGAGACC TACAACGACGAGTCTCGAATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGACTACAGA GAGTATGGCCGAAGGCTGGTCATGAGCATCAGGAGAAGTGCATTGATGTGA Clone variation with respect to NM_002945.3 12 a=>g >OriGene 5' read for NM_002945 unedited
TATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGGGACCCGGGTGGGGAAGCTG GAGCTGTTGCGGGGTCCGCGGGGAAGTCTTGGCGGTGGAGCCATGGTCGGCCAGCTGAGC GAGGGGGCCATTGCGGCCATCATGCAGAAGGGGGATACAAACATAAAGCCCATCCTCCAA GTCATCAACATCCGTCCCATTACTACGGGGAATAGTCCGCCGCGTTATCGACTGCTCATG AGTGATGGATTGAACACTCTATCCTCTTTCATGTTGGCGACACAGTTGAACCCTCTCGTG GAGGAAGAACAATTGTCCAGCAACTGTGTATGCCAGATTCACAGATTTATTGTGAACACT CTGAAAGACGGAAGGAGAGTAGTTATCTTGATGGATTTAGAAGTTNTGAAGTCAGCTGAA GCAGTTGGAGTGNAAGATTGGCAATCCAGTGCCCTATAATGAAGGACTCGGGCAGCCGCA AGTAGCTCCTCCAGCGCCAGCAGCCAGCCCAGCAGCAAGCAGCAGGCCCCAGCCGCAGAA TGGAAGCTGGGNAAATGGGTTCTACTGTTTCTAGGGCTTATGGTGCTTCAAAGACATTTG GAAAGGCTGCAGGTCCCAGCCTGTCACACACTTCTGGNGGAACACAGTCNAAAGTGGTGC CCATTGCCAGCCTCACTCCTTACCAGTCCAAAGTGACCCATTTGTGGCTCGTGTTACCAA CANAAGTCAAAATCCGTACCTGGAGCACTNCCGAAGGGGAAAAGGGAAAGCTTTTCTCCC TAGAACCTGNTGACAAAAAGTGGTGGAAATCCGAGCTACAGCTTTTCAATGAGCAGTGGG ACCAAGTTCTTTTCTCTTTTTTGGAGTGAACCAGGGGTGATTATTTTTTCGAAAGGCACC CTTGAGAATGCAAACAAGCGTTCACCAGCTGTTAAAATGACTACCAAATGACCTCATACG AAACTTCGTCTGCCTTGGAAGACACATATTACTCCGCGGTTAATTGATCTCCGGGGGAAA GA >OriGene 3' read for NM_002945 unedited
GGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACAACCAAAGATATA CTGTTTATTAAAACAAAGCAAGGGAAAACAAAAGGCTTCAAAATCCTTTCCATGTAACAT AGCTTTAGTCAAAAAAAACAAAATACATGAATGCAGGAATGTGTACAGGTAAAAAAAGTA CGCACTCACCAAGCGAAGCGCAGCATTAATTGAAGTGAAAAGTAAAGAAACGCTTTGTAG CCGCTCGGATTTCCTTAGGGGACAGCAAGTCAATGCTTATCAGCGGTCCTCAAAAGAGAC AATTTTGATTAATATCAGACCCATCTGACTCAGTCTATTAAACCCTGAAGGAAGGATACT CTTCAGATATAGAAGATATGCCTTTGATTAATCATTCTACCCTATTGCCATTCCAAGCAT TACCAACTATGTAGCACCTCTAGAAAGAGAGAGCCTTCGAATTCTAGGAAGACACACTTA CTAAAATCCCCAGGTGCTCATGACCGCTTCTCAGCTCGCCCCGCTGAGAAGAACCCAAGT TGTGAGCGGGATGGNGGGGGGGTTGTGNACACCACTGGAGAAGCAAAAACCTAGTTACAT AATTTACTTCATGGTCTGCAGTTAGGGTCAGTGACTTACGACATAATTCCTGCTTGATGA TAATGAAATTGACAGAAGNCCTGAGGCAGAGGGAGTGACTGGTCTTGCGCTAGCCACTGG CTGACTCCGTAGGCCTGACACAGTCTACACCACAACACCTGAGCGTATTTTCCTTTGCTA CCGATGGGGGTCTGANAGCGCACGAGGGNAGGAATTGCTTANTCCACATCAAGAGCCTCT GCACTGGGTACTACATGGGATCGTACACGGAGGGGGGAAGAAAATGATTCCTTCTGGCTA TTGCAAATTTCGCCCGATTGGCCGTGGTCTTTACATAAAGCAATTTTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002945 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002945.2, NP_002936.1 |
| RefSeq Size | 2824 bp |
| RefSeq ORF | 1851 bp |
| Locus ID | 6117 |
| UniProt ID | P27694 |
| Domains | tRNA_anti, Rep-A_N |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair |
| Gene Summary | This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202066 | RPA1 (Myc-DDK-tagged)-Human replication protein A1, 70kDa (RPA1) |
CNY 4,584.00 |
|
| RC202066L1 | Lenti ORF clone of Human replication protein A1, 70kDa (RPA1), Myc-DDK-tagged |
CNY 6,984.00 |
|
| RC202066L2 | Lenti ORF clone of Human replication protein A1, 70kDa (RPA1), mGFP tagged |
CNY 6,460.00 |
|
| RC202066L3 | Lenti ORF clone of Human replication protein A1, 70kDa (RPA1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202066L4 | Lenti ORF clone of Human replication protein A1, 70kDa (RPA1), mGFP tagged |
CNY 6,984.00 |
|
| RG202066 | RPA1 (tGFP-tagged) - Human replication protein A1, 70kDa (RPA1) |
CNY 6,184.00 |
|
| SC324660 | RPA1 (untagged)-Human replication protein A1, 70kDa (RPA1) |
CNY 4,592.00 |
