LATS1 (NM_004690) Human Untagged Clone

CAT#: SC323703

LATS1 (untagged)-Kinase deficient mutant (K734M) of Human LATS, large tumor suppressor, homolog 1 (Drosophila) (LATS1)



  "NM_004690" in other vectors (7)

CNY 21,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-LATS1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms WARTS; wts
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004690, the custom clone sequence may differ by one or more nucleotides


ATGAAGAGGAGTGAAAAGCCAGAAGGATATAGACAAATGAGGCCTAAGACCTTTCCTGCCAGTAACTATA
CTGTCAGTAGCCGGCAAATGTTACAAGAAATTCGGGAATCCCTTAGGAATTTATCTAAACCATCTGATGC
TGCTAAGGCTGAGCATAACATGAGTAAAATGTCAACCGAAGATCCTCGACAAGTCAGAAATCCACCCAAA
TTTGGGACGCATCATAAAGCCTTGCAGGAAATTCGAAACTCTCTGCTTCCATTTGCAAATGAAACAAATT
CTTCTCGGAGTACTTCAGAAGTTAATCCACAAATGCTTCAAGACTTGCAAGCTGCTGGATTTGATGAGGA
TATGGTTATACAAGCTCTTCAGAAAACTAACAACAGAAGTATAGAAGCAGCAATTGAATTCATTAGTAAA
ATGAGTTACCAAGATCCTCGACGAGAGCAGATGGCTGCAGCAGCTGCCAGACCTATTAATGCCAGCATGA
AACCAGGGAATGTGCAGCAATCAGTTAACCGCAAACAGAGCTGGAAAGGTTCTAAAGAATCCTTAGTTCC
TCAGAGGCATGGCCCGCCACTAGGAGAAAGTGTGGCCTATCATTCTGAGAGTCCCAACTCACAGACAGAT
GTAGGAAGACCTTTGTCTGGATCTGGTATATCAGCATTTGTTCAAGCTCACCCTAGCAACGGACAGAGAG
TGAACCCCCCACCACCACCTCAAGTAAGGAGTGTTACTCCTCCACCACCTCCAAGAGGCCAGACTCCCCC
TCCAAGAGGTACAACTCCACCTCCCCCTTCATGGGAACCAAACTCTCAAACAAAGCGCTATTCTGGAAAC
ATGGAATACGTAATCTCCCGAATCTCTCCTGTCCCACCTGGGGCATGGCAAGAGGGCTATCCTCCACCAC
CTCTCAACACTTCCCCCATGAATCCTCCTAATCAAGGACAGAGAGGCATTAGTTCTGTTCCTGTTGGCAG
ACAACCAATCATCATGCAGAGTTCTAGCAAATTTAACTTTCCATCAGGGAGACCTGGAATGCAGAATGGT
ACTGGACAAACTGATTTCATGATACACCAAAATGTTGTCCCTGCTGGCACTGTGAATCGGCAGCCACCAC
CTCCATATCCTCTGACAGCAGCTAATGGACAAAGCCCTTCTGCTTTACAAACAGGGGGATCTGCTGCTCC
TTCGTCATATACAAATGGAAGTATTCCTCAGTCTATGATGGTGCCAAACAGAAATAGTCATAACATGGAA
CTATATAACATTAGTGTACCTGGACTGCAAACAAATTGGCCTCAGTCATCTTCTGCTCCAGCCCAGTCAT
CCCCGAGCAGTGGGCATGAAATCCCTACATGGCAACCTAACATACCAGTGAGGTCAAATTCTTTTAATAA
CCCATTAGGAAATAGAGCAAGTCACTCTGCTAATTCTCAGCCTTCTGCTACAACAGTCACTGCAATTACA
CCAGCTCCTATTCAACAGCCTGTGAAAAGTATGCGTGTATTAAAACCAGAGCTACAGACTGCTTTAGCAC
CTACACACCCTTCTTGGATACCACAGCCAATTCAAACTGTTCAACCCAGTCCTTTTCCTGAGGGAACCGC
TTCAAATGTGACTGTGATGCCACCTGTTGCTGAAGCTCCAAACTATCAAGGACCACCACCACCCTACCCA
AAACATCTGCTGCACCAAAACCCATCTGTTCCTCCATACGAGTCAATCAGTAAGCCTAGCAAAGAGGATC
AGCCAAGCTTGCCCAAGGAAGATGAGAGTGAAAAGAGTTATGAAAATGTTGATAGTGGGGATAAAGAAAA
GAAACAGATTACAACTTCACCTATTACTGTTAGGAAAAACAAGAAAGATGAAGAGCGAAGGGAATCTCGT
ATTCAAAGTTATTCTCCTCAAGCATTTAAATTCTTTATGGAGCAACATGTAGAAAATGTACTCAAATCTC
ATCAGCAGCGTCTACATCGTAAAAAACAATTAGAGAATGAAATGATGCGGGTTGGATTATCTCAAGATGC
CCAGGATCAAATGAGAAAGATGCTTTGCCAAAAAGAATCTAATTACATCCGTCTTAAAAGGGCTAAAATG
GACAAGTCTATGTTTGTGAAGATAAAGACACTAGGAATAGGAGCATTTGGTGAAGTCTGTCTAGCAAGAA
AAGTAGATACTAAGGCTTTGTATGCAACAAAAACTCTTCGAAAGAAAGATGTTCTTCTTCGAAATCAAGT
CGCTCATGTTAAGGCTGAGAGAGATATCCTGGCTGAAGCTGACAATGAATGGGTAGTTCGTCTATATTAT
TCATTCCAAGATAAGGACAATTTATACTTTGTAATGGACTACATTCCTGGGGGTGATATGATGAGCCTAT
TAATTAGAATGGGCATCTTTCCAGAAAGTCTGGCACGATTCTACATAGCAGAACTTACCTGTGCAGTTGA
AAGTGTTCATAAAATGGGTTTTATTCATAGAGATATTAAACCTGATAATATTTTGATTGATCGTGATGGT
CATATTAAATTGACTGACTTTGGCCTCTGCACTGGCTTCAGATGGACACACGATTCTAAGTACTATCAGA
GTGGTGACCATCCACGGCAAGATAGCATGGATTTCAGTAATGAATGGGGGGATCCCTCAAGCTGTCGATG
TGGAGACAGACTGAAGCCATTAGAGCGGAGAGCTGCACGCCAGCACCAGCGATGTCTAGCACATTCTTTG
GTTGGGACTCCCAATTATATTGCACCTGAAGTGTTGCTACGAACAGGATACACACAGTTGTGTGATTGGT
GGAGTGTTGGTGTTATTCTTTTTGAAATGTTGGTGGGACAACCTCCTTTCTTGGCACAAACACCATTAGA
AACACAAATGAAGGTTATCAACTGGCAAACATCTCTTCACATTCCACCACAAGCTAAACTCAGTCCTGAA
GCTTCTGATCTTATTATTAAACTTTGCCGAGGACCCGAAGATCGCTTAGGCAAGAATGGTGCTGATGAAA
TAAAAGCTCATCCATTTTTTAAAACAATTGACTTCTCCAGTGACCTGAGACAGCAGTCTGCTTCATACAT
TCCTAAAATCACACACCCAACAGATACATCAAATTTTGATCCTGTTGATCCTGATAAATTATGGAGTGAT
GATAACGAGGAAGAAAATGTAAATGACACTCTCAATGGATGGTATAAAAATGGAAAGCATCCTGAACATG
CATTCTATGAATTTACCTTCCGAAGGTTTTTTGATGACAATGGCTACCCATATAATTATCCGAAGCCTAT
TGAATATGAATACATTAATTCACAAGGCTCAGAGCAGCAGTCGGATGAAGATGATCAAAACACAGGCTCA
GAGATTAAAAATCGCGATCTAGTATATGTTTAA


>OriGene 5' read for NM_004690 unedited
CCGCCCGTCTGAGCAATGGGCGGTAGGCGTGTACTGGACAGTGGAGGTCTATATAAGCAGAGCTCGTTTA
GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGGCGGCGG
CGGTGGCCTGGTGTTAAGGGGAGAGCCAGGTCCTTACGACCCCTGGGACGGGCCGCGCTGGCCCGCGGCA
GCCCCCCCGTTCGTCTCCCCGCTCTGCCCCACCAGGGATACTTGGGGTTGCTGGGACGGACTCTGGCCGC
CTCAGCGTCCGCCCTCAGGCCCGTGGCCGCTGTCCAGGAGCTCTGCTCTCCCCTCCAGAGTTAATTATTT
ATATTGTAAAGAATTTTAACAGTCCTGGGGACTTCCTTGAAGCATCATTTTCACTTTTGCTCAGAAGAAA
GCTCTGGATCTATCAAATAAAGAAGTCCTTCGTGTGGGCTACATATATAGATGTTTTCATGAAGAGGAGT
GAAAAGCCAGAAGGATATAGACAAATGAGGCCTAAGACCTTTCCTGCCAGTAACTATACTGTCAGTAGCC
GGCAAATGTTACAAGAAATTCGGGAATCCCTTAGGAATTTATCTAAACCATCTGATGCTGCTAAGGCTGA
GCATAACATGAGTAAAATGTCAACCGAAGATCCTCGACAAGTCAGAAATCCACCCAAATTTGGGACGCAT
CATAAGCCTTGCAGAAATTCGAAACTCTCTGCTTCCATTTGCAAATGAAACAAATTCTTCTCGGAGTACT
TCAGAAGTTAATCCACAAATGCTTCAGGACTTGCAGCTGCTGGATTTGATGAGATATGGGTTATACAGCT
CTTCAGAACTAACAACAGAAGTATAGAAGCAGCATTTGAATTCATTAGTAAAATGGAGTACCAGATCTCG
ACGAGAGCGAATGCTGCAGCGCTGCGACCTATTTAATGCCAGCATGAAACCAGGGATGTTGCCCGCATCA
GTTAG
>SC323703 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TYTCTCAGCATTTAATTCTTTATGGAGCAACATGTAGAAAATGTACTCAAATCTCATCAGCAGCGTCTAC
ATCGTAAAAAACAATTAGAGAATGAAATGATGCGGCTGATTTAAACTTTTATGGTGTTGGAAGCTTTGCT
GACTTCTGTAGTCTCCATTACTGTGTAGGTTGGATTATCTCAAGATGCCCAGGATCAAATGAGAAAGATG
CTTTGCCAAAAAGAATCTAATTACATCCGTCTTAAAAGGGCTAAA
Restriction Sites Please inquire     
ACCN NM_004690
Insert Size 4700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004690.2, NP_004681.1
RefSeq Size 4756 bp
RefSeq ORF 3393 bp
Locus ID 9113
UniProt ID O95835
Domains UBA, pkinase
Protein Families Druggable Genome, Protein Kinase
Gene Summary The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017]
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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