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ULK3 (NM_001099436) Human Untagged Clone
CAT＃: SC323667
ULK3 (untagged)-Kinase deficient mutant (K87M) of Human unc-51-like kinase 3 (C. elegans) (ULK3)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323667 sequence for NM_001099436 edited (data generated by NextGen Sequencing)
ATGGCGGGGCCCGGCTGGGGTCCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTG GGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTG GTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTC ACGGAGATTGAGATCCTCAAGGGCATTCGACATCCCCACATTGTGCAGCTGAAAGACTTT CAGTGGGACAGTGACAATATCTACCTCATCATGGAGTTTTGCGCAGGGGGCGACCTGTCT CGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGTGTCTTCATGCAGCAA TTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAGCCA CAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTC GCACAACACATGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATG GCCCCCGAGATGGTGTGCCAGCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGG GTCATCCTGTATGAAGCCCTCTTCGGGCAGCCCCCCTTTGCCTCCAGGTCGTTCTCGGAG CTGGAAGAGAAGATCCGTAGCAACCGGGTCATCGAGCTCCCCTTGCGGCCCCTGCTCTCC CGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGGGACCCCAGCCGTCGCATCTCC TTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGT CTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGATTCA GCAGCTGCCTTATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTAT GAAGTGGATGCCCAGCGGAAGGAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGG GCTGAGGAGCTCAAGGCCATCGTCTCCTCTTCCAATCAGGCCCTGCTGAGGCAGGGGACC TCTGCCCGAGACCTGCTCAGAGAGATGGCCCGGGACAAGCCACGCCTCCTAGCTGCCCTG GAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAGGCCGCCGGCGGGGAGCAGGATGCCCTG GACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTGGCAGCGGAGCCCCCGGGC CGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCTGAATACTTG AAGGAGCAGGTCAAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGA CTGTCGGAATCTGTTCGTAGCTCTTGCACCCTTCAGTGA Clone variation with respect to NM_001099436.1 131 a=>t >OriGene 5' read for mutant NM_001099436 unedited
CCCGCCCGTCCCGCAACGGGCGGTAGGCGCTGTACGGTTGGGAGGTTCATATAAGCAGAGCTCGTTTAGT GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGCGCCTGGAC GGCTTCATCCTCACCGAGCGCCTGGGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGG ACACTCGTGAAGTGGTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCT CCTCACGGAGATTGAGATCCTCAAGGGCATTCGACATCCCCACATTGTGCAGCTGAAAGACTTTCAGTGG GACAGTGACAATATCTACCTCATCATGGGAGTTTTGCGCAGGGGGCGACCCTGTCTCGCTTCATCCCATA CCCCGCAGGATTTCTGCCTGAAAAGGTGCCGCGGTGTCTTCATGCAGCATTTAGCTAGCCGCCCCTGCAT TCCTGCATGAAACGGAATTATCCTCTCACCTGATCCGGAGCCCCAGAACATCTACTGACCTCCTGGAAAA GCCCACCTAAAACTGGCAAACTTTGGTTTGCAACACACAGGTCCCTTGGTAGAAGCCTTCCCCGGGTCTC CCCTCTAATGCCCCAATTGGTGCCACGCGATTAGACCCCTTGACTTTGTCCAGGGGGCTCTCGTGTAAGC CTTTGGCAGCCCTTGCCCAGTGTCTCTGACTGAAGAATCTGAAACGGTATAGTCCTTGGCCTGTTCGAAT CGCACTCGACGCCTGCGGACAGCGTATATTGATTGACTGACGATCCTTGGACTGCAACCGTTGACGA >SC323667 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGCGCCTGGACGGCTTCAT CCTCACCGAGCGCCTGGGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGT GAAGTGGTAGCCATAATGTGTGTAGCCAAGAAAAGTCTGAACAAG |
| Restriction Sites | Please inquire |
| ACCN | NM_001099436 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001099436.1, NP_001092906.1 |
| RefSeq Size | 2635 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 25989 |
| UniProt ID | Q6PHR2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | mTOR signaling pathway, Regulation of autophagy |
| Gene Summary | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220209 | ULK3 (Myc-DDK-tagged)-Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 3,656.00 |
|
| RC220209L1 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC220209L2 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), mGFP tagged |
CNY 5,890.00 |
|
| RC220209L3 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220209L4 | Lenti ORF clone of Human unc-51-like kinase 3 (C. elegans) (ULK3), mGFP tagged |
CNY 5,890.00 |
|
| RG220209 | ULK3 (tGFP-tagged) - Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 4,370.00 |
|
| SC316533 | ULK3 (untagged)-Human unc-51-like kinase 3 (C. elegans) (ULK3) |
CNY 7,220.00 |
