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MAP3K8 (NM_005204) Human Untagged Clone
CAT＃: SC323633
MAP3K8 (untagged)-Kinase deficient mutant (D253A) of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8)
CN¥ 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AURA2; c-COT; COT; EST; ESTF; MEKK8; Tpl-2; TPL2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323633 sequence for NM_005204 edited (data generated by NextGen Sequencing)
ATGGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTTATTAATTAAACAT TTAAATGTGTCTGATGTAATAGACATTATGGAAAATCTTTATGCAAGTGAAGAGCCAGCA GTTTATGAACCCAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCT AAGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATACGGAACT GTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTATGGA CAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCCCCAAAATGGACGT TACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGT TCTGATTTTATTCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCACAAGATATAAAGACG AAGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCTGATGTGGAA ATCCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGGGGT GAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCTGGAGAAACTGGAG AGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGA CTTGATTTTCTACACTCAAAGAAAGTGATCCATCATGCCATTAAACCTAGCAACATTGTT TTCATGTCCACAAAAGCTGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGAT GTCTATTTTCCTAAGGACCTCCGAGGAACAGAGATTTACATGAGCCAAGAGGTCATCCTG TGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCACGCTCATCCACATG CAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTG TACATAATCCACAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGG ATGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCA GACCTACTAAAACATGAGGCCCTGAACCCGCCGAGAGAGGATCAGCCACGCTGTCAGAGT CTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGAGCTGGAACTTCCT GAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCGAGGAATCTGAGATGCTCAAGAGG CAACGCTCTCTCTACATCGACCTCGGCGCTCTGGCTGGCTACTTCAATCTTGTTCGGGGA CCACCAACGCTTGAATATGGCTGA Clone variation with respect to NM_005204.3 234 t=>c;758 a=>c;759 t=>c;887 c=>a;1173 c=>g >OriGene 5' read for mutant NM_005204 unedited
CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGGAAGCGGGGA CCCGCTGTCACTGCGCCTCCCGCTGCCGACGCCGCCTGGACGGCCGCACTCTCCCTGCCCGAGACCCGAT GCAATCTTCTTACCGCGAAGAAGCCAGGGGAATAGGTAGCCACATCTTGTTTGCAGATAAGAAAGGAAGC TAACGCAGTATCTGCAAAGCCAGGAGTCTGACTCAGTACTTTTTCTCACTCATGCATACAAAGCAGCTAA GAAATGACACAGCTTTATTTTACCATGCCCCCTTGACACTGGCACTGGAGCACTTTTATGAGCCTGGACC TCGGGTTAATTCCTCCACAACCCCCCTCAGGAAAACTCCCCAGGAAAAAAGCACCAGGTATGGGAATACA CATAGCACACTGAAGTTTGAAAAATAAGAAAAGAAAATGGATTTATTTAATTAAAACATTAAAAGTGGTG TTGAGAGGTAATAAAACTTTAGGGAAAAATCTTTTGGCCGTGTAAAGACCCGCCATTTTAAAGACCCCAG TCAAAGAGCATAGTGGTCAAGACGGTACCAACCGGATGGAGCTTTTTAGCTCCTGCTGGGGATTGGGGCC AAAAAGGCCCCGGGGGTTGGTAACATTTAAATACCAGCACTGGGGGGGGTTTTGTGTTTTTTTTTGACAC CCTATTCCCCAACCGGTGAAAATTTTTTTTGGGGGCCCACCCCCCGGGGCTTGGGGTATTTTTATAACGT GGGGCCCTCCCCCCCCAGGAGGGGTCTTCACAAAATACCTACGGTGTCCTCGTACACCCGCGAGCAGACT TTCTAGAGAGATATGTGTGCACTATTCATCGCGCGCGCCTCGTGAGATGTCTGTCTGCGCATATAGCAGA CAAAAGCGTGTCAGATCGCTCCATCAGTGATCACTGCTGCATGATGAGCAGCGCGTGCG >SC323633 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GWCTGGACTGMTTAAGGATATTGGTTCTGATTTTATTCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCA CAAGATATAAAGACGAAGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCTGATG TGGAAATCCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGGGGTGAAAC TGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCTGGA |
| Restriction Sites | Please inquire |
| ACCN | NM_005204 |
| Insert Size | 2870 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005204.2, NP_005195.2 |
| RefSeq Size | 3096 bp |
| RefSeq ORF | 1404 bp |
| Locus ID | 1326 |
| UniProt ID | P41279 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway |
| Gene Summary | This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011] Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211171 | MAP3K8 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8) |
CN¥ 5,488.00 |
|
| RC211171L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8), Myc-DDK-tagged |
CN¥ 7,888.00 |
|
| RC211171L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8), mGFP tagged |
CN¥ 5,890.00 |
|
| RC211171L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8), Myc-DDK-tagged |
CN¥ 7,888.00 |
|
| RC211171L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8), mGFP tagged |
CN¥ 7,888.00 |
|
| RG211171 | MAP3K8 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8) |
CN¥ 7,088.00 |
|
| SC112674 | MAP3K8 (untagged)-Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8) |
CN¥ 5,488.00 |
