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LOK (STK10) (NM_005990) Human Untagged Clone
CAT＃: SC323467
STK10 (untagged)-Kinase deficient mutant (K65M) of Human serine/threonine kinase 10 (STK10)
CNY 7,096.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LOK; PRO2729 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323467 sequence for NM_005990 edited (data generated by NextGen Sequencing)
ATGGCTTTTGCCAATTTCCGCCGCATCCTGCGCCTGTCTACCTTCGAGAAGAGAAAGTCC CGCGAATATGAGCACGTCCGCCGCGACCTGGACCCCAACGAGGTGTGGGAGATCGTGGGC GAGCTGGGCGACGGCGCCTTCGGCAAGGTTTACAAGGCCAAGAATAAGGAGACGGGTGCT TTGGCTGCGGCCATGGTCATTGAAACCAAGAGTGAGGAGGAGCTGGAGGACTACATCGTG GAGATTGAGATCCTGGCCACCTGCGACCACCCCTACATTGTGAAGCTCCTGGGAGCCTAC TATCACGACGGGAAGCTGTGGATCATGATTGAGTTCTGTCCAGGGGGAGCCGTGGACGCC ATCATGCTGGAGCTGGACAGAGGCCTCACGGAGCCCCAGATACAGGTGGTTTGCCGCCAG ATGCTAGAAGCCCTCAACTTCCTGCACAGCAAGAGGATCATCCACCGAGATCTGAAAGCT GGCAACGTGCTGATGACCCTCGAGGGAGACATCAGGCTGGCTGACTTTGGTGTGTCTGCC AAGAATCTGAAGACTCTACAGAAACGAGATTCCTTCATCGGCACGCCTTACTGGATGGCC CCCGAGGTGGTCATGTGTGAGACCATGAAAGACACGCCCTACGACTACAAAGCCGACATC TGGTCCCTGGGCATCACGCTGATTGAGATGGCCCAGATCGAGCCGCCACACCACGAGCTC AACCCCATGCGGGTCCTGCTAAAGATCGCCAAGTCAGACCCTCCCACGCTGCTCACGCCC TCCAAGTGGTCTGTAGAGTTCCGTGACTTCCTGAAGATAGCCCTGGATAAGAACCCAGAA ACCCGACCCAGTGCCGCGCAGCTGCTGGAGCATCCCTTCGTCAGCAGCATCACCAGTAAC AAGGCTCTGCGGGAGCTGGTGGCTGAGGCCAAGGCCGAGGTGATGGAAGAGATCGAAGAC GGCCGGGATGAGGGGGAAGAGGAGGACGCCGTGGATGCCGCCTCCACCCTGGAGAACCAT ACTCAGAACTCCTCTGAGGTGAGTCCGCCAAGCCTCAATGCTGACAAGCCTCTCGAGGAG TCACCTTCCACCCCGCTGGCACCCAGCCAGTCTCAGGACAGTGTGAATGAGCCCTGCAGC CAGCCCTCTGGGGACAGATCCCTCCAAACCACCAGTCCCCCAGTCGTGGCCCCTGGAAAT GAGAACGGCCTGGCAGTGCCTGTGCCCCTGCGGAAGTCCCGACCCGTGTCAATGGATGCC AGAATTCAGGTAGCCCAGGAGAAGCAAGTTGCTGAGCAGGGTGGGGACCTCAGCCCAGCA GCCAACAGATCTCAAAAGGCCAGCCAGAGCCGGCCCAACAGCAGCGCCCTGGAGACCTTG GGTGGGGAGAAGCTGGCCAATGGCAGCCTGGAGCCACCTGCCCAGGCAGCTCCAGGGCCT TCCAAGAGGGACTCGGACTGCAGCAGCCTCTGCACCTCTGAGAGCATGGACTATGGTACC AATCTCTCCACTGACCTGTCGCTGAACAAAGAGATGGGCTCTCTGTCCATCAAGGACCCG AAACTGTACAAAAAAACCCTCAAGCGGACACGCAAATTTGTGGTGGATGGTGTGGAGGTG AGCATCACCACCTCCAAGATCATCAGCGAAGATGAGAAGAAGGATGAGGAGATGAGATTT CTCAGGCGCCAGGAACTCCGAGAGCTTCGGCTGCTCCAGAAAGAAGAGCATCGGAACCAG ACCCAGCTGAGTAACAAGCATGAGCTGCAGCTGGAGCAAATGCATAAACGTTTTGAACAG GAAATCAACGCCAAGAAGAAGTTCTTTGACACGGAATTAGAGAACCTGGAGCGTCAGCAA AAGCAGCAAGTGGAGAAGATGGAGCAAGACCATGCCGTGCGCCGCCGGGAGGAGGCCAGG CGGATCCGCCTGGAGCAGGATCGGGACTACACCAGGTTCCAAGAGCAGCTCAAACTGATG AAGAAAGAGGTGAAGAACGAGGTGGAGAAGCTCCCCCGACAGCAGCGGAAGGAAAGCATG AAGCAGAAGATGGAGGAGCACACGCAGAAAAAGCAGCTTCTTGACCGGGACTTTGTAGCC AAGCAGAAGGAGGACCTGGAGCTGGCCATGAAGAGGCTCACCACCGACAACAGGCGGGAG ATCTGTGACAAGGAGCGCGAGTGCCTCATGAAGAAGCAGGAGCTCCTTCGAGACCGGGAA GCAGCCCTGTGGGAGATGGAAGAGCACCAGCTGCAGGAGAGGCACCAGCTGGTGAAGCAG CAGCTCAAAGACCAGTACTTCCTCCAGCGGCACGAGCTGCTGCGCAAGCATGAGAAGGAG CGGGAGCAGATGCAGCGCTACAACCAGCGCATGATAGAGCAGCTGAAGGTGCGGCAGCAA CAGGAAAAGGCGCGGCTGCCCAAGATCCAGAGGAGTGAGGGCAAGACGCGCATGGCCATG TACAAGAAGAGCCTCCACATCAACGGCGGGGGCAGCGCAGCTGAGCAGCGTGAGAAGATC AAGCAGTTCTCCCAGCAGGAGGAGAAGAGGCAGAAGTCGGAGCGGCTGCAGCAACAGCAG AAACACGAGAACCAGATGCGGGACATGCTGGCGCAGTGTGAGAGCAACATGAGCGAGCTG CAGCAGCTGCAGAATGAAAAGTGCCACCTCCTGGTAGAGCACGAAACCCAGAAACTGAAG GCCCTGGATGAGAGCCATAACCAGAACCTGAAGGAATGGCGGGACAAGCTTCGGCCGCGC AAGAAGGCTCTGGAAGAGGATCTGAACCAGAAGAAGCGGGAGCAGGAGATGTTCTTCAAG CTGAGCGAGGAGGCGGAGTGCCCAAACCCCTCCACCCCAAGCAAGGCCGCCAAGTTCTTC CCCTACAGTTCTGCGGATGCTTCTTAA Clone variation with respect to NM_005990.3 194 a=>t;195 a=>g;756 g=>a;783 t=>c >OriGene 5' read for mutant NM_005990 unedited
ACCCCCCGTCCCGCAACGGGGCGGAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATCCGGCACGAGGCGGCACAAGGGAG GCGGCCCTGGGGACCTGACAGGGCGGGGGCGGGGGCGGGCACGCCGCCGCCTGCGCGGTTCCGGGTGCTC CCGGCGCGGCGCTGCCCAGGCACACTTGACCGAAGCCGGGCTGCGGGGAAAGGCAGGTCTGGGCAGAAGG CGGACACGCTTCTGCGGTCCGTGCTTGGGCGATAGTCCGGACACGAAGGCGAGGAGAGGCGTAAGACAAC CCAAGACTTTTCGCGATGGTAATAAAGGGAACTGCTGGATTGGTGGTAACAGACCGGAATAAGTAGGGCA GAATCCAAGCAAAATAGCCAAATCCGGAAAAAAAACTAACACCCCTTTAAAAATCCCGAAAACGTTAGGT CGTCCCCCGGCTATCTT >SC323467 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CAATKCACCACGAGGTKGGGAGWCGTGGGCGAGCTGGGCGACGGCGCCTTCGGCAAGGTTTACAAGGCCA AGAATAAGGAGACGGGTGCTTTGGCTGCGGCCATGGTCATTGAAACCAAGAGTGAGGAGGAGCTGGAGGA CTACATCGTGGAGATTGAGATCCTGGCCACCTGCGACCACCCCTACATTGTGAAGCTCCTGGGAGCCTAC TATCACGACGGGAAGCTGTGGATCATGATTGAGTTCTGTCCAGGG |
| Restriction Sites | Please inquire |
| ACCN | NM_005990 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005990.2, NP_005981.2 |
| RefSeq Size | 4398 bp |
| RefSeq ORF | 2907 bp |
| Locus ID | 6793 |
| UniProt ID | O94804 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209827 | STK10 (Myc-DDK-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 7,088.00 |
|
| RC209827L1 | Lenti-ORF clone of STK10 (Myc-DDK-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 9,488.00 |
|
| RC209827L2 | Lenti-ORF clone of STK10 (mGFP-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 5,890.00 |
|
| RC209827L3 | Lenti-ORF clone of STK10 (Myc-DDK-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 5,890.00 |
|
| RC209827L4 | Lenti-ORF clone of STK10 (mGFP-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 5,890.00 |
|
| RG209827 | STK10 (tGFP-tagged) - Human serine/threonine kinase 10 (STK10) |
CNY 8,688.00 |
|
| SC116411 | STK10 (untagged)-Human serine/threonine kinase 10 (STK10) |
CNY 7,096.00 |
