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LOK (STK10) (NM_005990) Human Tagged ORF Clone
CAT＃: RC209827
STK10 (Myc-DDK-tagged)-Human serine/threonine kinase 10 (STK10)
ORF Plasmid: tGFP
"NM_005990" in other vectors (7)
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CNY 7,088.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LOK; PRO2729 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209827 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTTGCCAATTTCCGCCGCATCCTGCGCCTGTCTACCTTCGAGAAGAGAAAGTCCCGCGAATATG AGCACGTCCGCCGCGACCTGGACCCCAACGAGGTGTGGGAGATCGTGGGCGAGCTGGGCGACGGCGCCTT CGGCAAGGTTTACAAGGCCAAGAATAAGGAGACGGGTGCTTTGGTTGCGGCCAAAGTCATTGAAACCAAG AGTGAGGAGGAGCTGGAGGACTACATCGTGGAGATTGAGATCCTGGCCACCTGCGACCACCCCTACATTG TGAAGCTCCTGGGAGCCTACTATCACGACGGGAAGCTGTGGATCATGATTGAGTTCTGTCCAGGGGGAGC CGTGGACGCCATCATGCTGGAGCTGGACAGAGGCCTCACGGAGCCCCAGATACAGGAGGTTTGCCGCCAG ATGCTAGAAGCCCTCAACTTCCTGCACAGCAAGAGGATCATCCACCGAGATCTGAAAGCTGGCAACGTGC TGATGACCCTCGAGGGAGACATCAGGCTGGCTGACTTTGGTGTGTCTGCCAAGAATCTGAAGACTCTACA GAAACGAGATTCCTTCATCGGCACGCCTTACTGGATGGCCCCCGAGGTGGTCATGTGTGAGACCATGAAA GACACGCCCTACGACTACAAAGCCGACATCTGGTCCCTGGGCATCACGCTGATTGAGATGGCCCAGATCG AGCCGCCACACCACGAGCTCAACCCCATGCGGGTCCTGCTAAAGATCGCCAAGTCAGACCCTCCCACGCT GCTCACGCCCTCCAAGTGGTCTGTAGAGTTCCGTGACTTCCTGAAGATAGCCCTGGATAAGAACCCAGAA ACCCGACCCAGTGCCGCGCAGCTGCTGGAGCATCCCTTCGTCAGCAGCATCACCAGTAACAAGGCTCTGC GGGAGCTGGTGGCTGAGGCCAAGGCCGAGGTGATGGAAGGGATCGAAGACGGCCGGGATGAGGGGGAAGA GGAGGACGCCGTGGATGCCGCCTCCACCCTGGAGAACCATACTCAGAACTCCTCTGAGGTGAGTCCGCCA AGCCTCAATGCTGACAAGCCTCTCGAGGAGTCACCTTCCACCCCGCTGGCACCCAGCCAGTCTCAGGACA GTGTGAATGAGCCCTGCAGCCAGCCCTCTGGGGACAGATCCCTCCAAACCACCAGTCCCCCAGTCGTGGC CCCTGGAAATGAGAACGGCCTGGCAGTGCCTGTGCCCCTGCGGAAGTCCCGACCCGTGTCAATGGATGCC AGAATTCAGGTAGCCCAGGAGAAGCAAGTTGCTGAGCAGGGTGGGGACCTCAGCCCAGCAGCCAACAGAT CTCAAAAGGCCAGCCAGAGCCGGCCCAACAGCAGCGCCCTGGAGACCTTGGGTGGGGAGAAGCTGGCCAA TGGCAGCCTGGAGCCACCTGCCCAGGCAGCTCCAGGGCCTTCCAAGAGGGACTCGGACTGCAGCAGCCTC TGCACCTCTGAGAGCATGGACTATGGTACCAATCTCTCCACTGACCTGTCGCTGAACAAAGAGATGGGCT CTCTGTCCATCAAGGACCCGAAACTGTACAAAAAAACCCTCAAGCGGACACGCAAATTTGTGGTGGATGG TGTGGAGGTGAGCATCACCACCTCCAAGATCATCAGCGAAGATGAGAAGAAGGATGAGGAGATGAGATTT CTCAGGCGCCAGGAACTCCGAGAGCTTCGGCTGCTCCAGAAAGAAGAGCATCGGAACCAGACCCAGCTGA GTAACAAGCATGAGCTGCAGCTGGAGCAAATGCATAAACGTTTTGAACAGGAAATCAACGCCAAGAAGAA GTTCTTTGACACGGAATTAGAGAACCTGGAGCGTCAGCAAAAGCAGCAAGTGGAGAAGATGGAGCAAGAC CATGCCGTGCGCCGCCGGGAGGAGGCCAGGCGGATCCGCCTGGAGCAGGATCGGGACTACACCAGGTTCC AAGAGCAGCTCAAACTGATGAAGAAAGAGGTGAAGAACGAGGTGGAGAAGCTCCCCCGACAGCAGCGGAA GGAAAGCATGAAGCAGAAGATGGAGGAGCACACGCAGAAAAAGCAGCTTCTTGACCGGGACTTTGTAGCC AAGCAGAAGGAGGACCTGGAGCTGGCCATGAAGAGGCTCACCACCGACAACAGGCGAGAGATCTGTGACA AGGAGCGCGAGTGCCTCATGAAGAAGCAGGAGCTCCTTCGAGACCGGGAAGCAGCCCTGTGGGAGATGGA AGAGCACCAGCTGCAGGAGAGGCACCAGCTGGTGAAGCAGCAGCTCAAAGACCAGTACTTCCTCCAGCGG CACGAGCTGCTGCGCAAGCATGAGAAGGAGCGGGAGCAGATGCAGCGCTACAACCAGCGCATGATAGAGC AGCTGAAGGTGCGGCAGCAACAGGAAAAGGCGCGGCTGCCCAAGATCCAGAGGAGTGAGGGCAAGACGCG CATGGCCATGTACAAGAAGAGCCTCCACATCAACGGCGGGGGCAGCGCAGCTGAGCAGCGTGAGAAGATC AAGCAGTTCTCCCAGCAGGAGGAGAAGAGGCAGAAGTCGGAGCGGCTGCAGCAACAGCAGAAACACGAGA ACCAGATGCGGGACATGCTGGCGCAGTGTGAGAGCAACATGAGCGAGCTGCAGCAGCTGCAGAATGAAAA GTGCCACCTCCTGGTAGAGCACGAAACCCAGAAACTGAAGGCCCTGGATGAGAGCCATAACCAGAACCTG AAGGAATGGCGGGACAAGCTTCGGCCGCGCAAGAAGGCTCTGGAAGAGGATCTGAACCAGAAGAAGCGGG AGCAGGAGATGTTCTTCAAGCTGAGCGAGGAGGCGGAGTGCCCAAACCCCTCCACCCCAAGCAAGGCTGC CAAGTTCTTCCCCTACAGTTCTGCGGATGCTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209827 protein sequence
Red=Cloning site Green=Tags(s) MAFANFRRILRLSTFEKRKSREYEHVRRDLDPNEVWEIVGELGDGAFGKVYKAKNKETGALVAAKVIETK SEEELEDYIVEIEILATCDHPYIVKLLGAYYHDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQEVCRQ MLEALNFLHSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVMCETMK DTPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPE TRPSAAQLLEHPFVSSITSNKALRELVAEAKAEVMEGIEDGRDEGEEEDAVDAASTLENHTQNSSEVSPP SLNADKPLEESPSTPLAPSQSQDSVNEPCSQPSGDRSLQTTSPPVVAPGNENGLAVPVPLRKSRPVSMDA RIQVAQEKQVAEQGGDLSPAANRSQKASQSRPNSSALETLGGEKLANGSLEPPAQAAPGPSKRDSDCSSL CTSESMDYGTNLSTDLSLNKEMGSLSIKDPKLYKKTLKRTRKFVVDGVEVSITTSKIISEDEKKDEEMRF LRRQELRELRLLQKEEHRNQTQLSNKHELQLEQMHKRFEQEINAKKKFFDTELENLERQQKQQVEKMEQD HAVRRREEARRIRLEQDRDYTRFQEQLKLMKKEVKNEVEKLPRQQRKESMKQKMEEHTQKKQLLDRDFVA KQKEDLELAMKRLTTDNRREICDKERECLMKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQR HELLRKHEKEREQMQRYNQRMIEQLKVRQQQEKARLPKIQRSEGKTRMAMYKKSLHINGGGSAAEQREKI KQFSQQEEKRQKSERLQQQQKHENQMRDMLAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQNL KEWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAECPNPSTPSKAAKFFPYSSADAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005990 |
| ORF Size | 2904 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005990.2, NP_005981.2 |
| RefSeq Size | 6039 bp |
| RefSeq ORF | 2907 bp |
| Locus ID | 6793 |
| UniProt ID | O94804 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 112.1 kDa |
| Gene Summary | This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209827L1 | Lenti-ORF clone of STK10 (Myc-DDK-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 9,488.00 |
|
| RC209827L2 | Lenti-ORF clone of STK10 (mGFP-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 5,890.00 |
|
| RC209827L3 | Lenti-ORF clone of STK10 (Myc-DDK-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 5,890.00 |
|
| RC209827L4 | Lenti-ORF clone of STK10 (mGFP-tagged)-Human serine/threonine kinase 10 (STK10) |
CNY 5,890.00 |
|
| RG209827 | STK10 (tGFP-tagged) - Human serine/threonine kinase 10 (STK10) |
CNY 8,688.00 |
|
| SC116411 | STK10 (untagged)-Human serine/threonine kinase 10 (STK10) |
CNY 7,096.00 |
|
| SC323467 | STK10 (untagged)-Kinase deficient mutant (K65M) of Human serine/threonine kinase 10 (STK10) |
CNY 7,096.00 |
