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CDK7 (NM_001799) Human Untagged Clone
CAT＃: SC323465
CDK7 (untagged)-Kinase deficient mutant (K41M) of Human cyclin-dependent kinase 7 (CDK7)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAK; CAK1; CDKN7; HCAK; MO15; p39MO15; STK1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323465 sequence for NM_001799 edited (data generated by NextGen Sequencing)
ATGGCTCTGGACGTGAAGTCTCGGGCAAAGCGTTATGAGAAGCTGGACTTCCTTGGGGAG GGACAGTTTGCCACCGTTTACAAGGCCAGAGATAAGAACACCAACCAAATTGTCGCCATT ATGAAAATCAAACTTGGACATAGATCAGAAGCTAAAGATGGTATAAATAGAACCGCCTTA AGAGAGATAAAATTATTACAGGAGCTAAGTCATCCAAATATAATTGGTCTCCTTGATGCT TTTGGACATAAATCTAATATTAGCCTTGTCTTTGATTTTATGGAAACTGATCTAGAGGTT ATAATAAAGGATAATAGTCTTGTGCTGACACCATCACACATCAAAGCCTACATGTTGATG ACTCTTCAAGGATTAGAATATTTACATCAACATTGGATCCTACATAGGGATCTGAAACCA AACAACTTGTTGCTAGATGAAAATGGAGTTCTAAAACTGGCAGATTTTGGCCTGGCCAAA TCTTTTGGGAGCCCCAATAGAGCTTATACACATCAGGTTGTAACCAGGTGGTATCGGGCC CCCGAGTTACTATTTGGAGCTAGGATGTATGGTGTAGGTGTGGACATGTGGGCTGTTGGC TGTATATTAGCAGAGTTACTTCTAAGGGTTCCTTTTTTGCCAGGAGATTCAGACCTTGAT CAGCTAACAAGAATATTTGAAACTTTGGGCACACCAACTGAGGAACAGTGGCCGGACATG TGTAGTCTTCCAGATTATGTGACATTTAAGAGTTTCCCTGGAATACCTTTGCATCACATC TTCAGTGCAGCAGGAGACGACTTACTAGATCTCATACAAGGCTTATTCTTATTTAATCCA TGTGCTCGAATTACGGCCACACAGGCACTGAAAATGAAGTATTTCAGTAATCGGCCAGGG CCAACACCTGGATGTCAGCTGCCAAGACCAAACTGTCCAGTGGAAACCTTAAAGGAGCAA TCAAATCCAGCTTTGGCAATAAAAAGGAAAAGAACAGAGGCCTTAGAACAAGGAGGATTG CCCAAGAAACTAATTTTTTAA Clone variation with respect to NM_001799.3 122 a=>t >OriGene 5' read for mutant NM_001799 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGTAGCTTTAAATT CGTGTTGTCCTGGGAGCTCGCCCTTTTCGGCTGGAGTCGGGCTTTACGGCGCCGGATGGCTCTGGACGTG AAGTCTCGGGCAAAGCGTTATGAGAAGCTGGACTTCCTTGGGGAGGGACAGTTTGCCACCGTTTACAAGG CCAGAGATAAAGAACACCAACCAAATTGTCGCCATTATGAAAATCAAAACTTGGACATAGATCAGAAGCT AAAGATGGTATAAATAGAACCGCCTTAAGAGAGATAAAATTATTACAGGACCTAAGTCTTCCAAATATAA TTGTCCTCCTGGATGCTTTTGAACTTAATTCTAATATAACCCTGTTCTTTGATTTTATGGAACCGATTCT AAAGGTTATAATAAAGATTAATGTCCTTGGGCTGACCCCTCCCCCCTCAAGCCCTCATGTGGTAAATCTT CAAAGATTTGGAAAATAAATCAAAAATGATACTAATAGGGGATCTAAAAAAAAAAACTTTTGCGGAAAAA AATAGGGTCAAACTCGGGGAATGGCCTGGCCATCTTTTTGAGCCCCCATGGTCTAACCCTTGGGTACCCG TGTATGGGCCCCCGGACTCTTTTACACGAGATTGTGTTGTGGGGCCCTTGCGCTTCTGCTAATAACAAAC TCCAAAGGGCTTTTGCGCGATATACTCTGTCTCAAAATTTTACTCTGCGCACCAGGACAGGGCGCAGCTA GTCCACATAACTAAAATCGGACACTTCACTCTGCGGGAGACATATATCACGAGTGTTATCGG >SC323465 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGTAGCTTTAAATTCGTGT TGTCCTGGGAGCTCGCCCTTTTCGGCTGGAGTCGGGCTTTACGGCGCCGGATGGCTCTGGACGTGAAGTC TCGGGCAAAGCGTTATGAGAAGCTGGACTTCCTTGGGGAGGGACA |
| Restriction Sites | Please inquire |
| ACCN | NM_001799 |
| Insert Size | 1280 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001799.2, NP_001790.1 |
| RefSeq Size | 1427 bp |
| RefSeq ORF | 1041 bp |
| Locus ID | 1022 |
| UniProt ID | P50613 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Cell cycle, Nucleotide excision repair |
| Gene Summary | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202736 | CDK7 (Myc-DDK-tagged)-Human cyclin-dependent kinase 7 (CDK7) |
CNY 5,488.00 |
|
| RC202736L1 | Lenti ORF clone of Human cyclin-dependent kinase 7 (CDK7), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC202736L2 | Lenti ORF clone of Human cyclin-dependent kinase 7 (CDK7), mGFP tagged |
CNY 5,890.00 |
|
| RC202736L3 | Lenti ORF clone of Human cyclin-dependent kinase 7 (CDK7), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC202736L4 | Lenti ORF clone of Human cyclin-dependent kinase 7 (CDK7), mGFP tagged |
CNY 7,888.00 |
|
| RG202736 | CDK7 (tGFP-tagged) - Human cyclin-dependent kinase 7 (CDK7) |
CNY 7,088.00 |
|
| SC119024 | CDK7 (untagged)-Human cyclin-dependent kinase 7 (CDK7) |
CNY 5,488.00 |
