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DIS3 (NM_001128226) Human Untagged Clone
CAT＃: SC323238
DIS3 (untagged)-Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 2
CNY 17,860.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2810028N01Rik; dis3p; EXOSC11; KIAA1008; RRP44 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001128226, the custom clone sequence may differ by one or more nucleotides
ATGCTCAAGTCCAAGACGTTCTTAAAAAAGACCCGGGCGGGCGGCGTGATGAAGATCGTG CGCGAGCACTACCTGCGAGACGACATCGGCTGCGGTGCGCCCGGGTGCGCAGCGTGTGGA GGGGCGCACGAGGGGCCGGCCCTGGAGCCGCAGCCCCAGGACCCGGCGAGCAGCGTCTGC CCGCAACCGCACTACTTGCTGCCCGACACTAATGTGTTACTGCACCAGATTGTAAGTGCC TGGAGGCCGGGGACCTGGGCTTCTGTGGCCTCCAGCCTGCGACTCCCAGGCAGCTTAGAA ACCTATGTAGAACAAGAACAGGGAGAAAATGCTAATGACAGGAATGATAGAGCGATTCGA GTAGCAGCAAAATGGTACAATGAACATTTGAAAAAAATGTCAGCAGACAACCAGCTGCAA GTTATCTTCATAACAAATGACAGGAGAAACAAAGAGAAAGCCATAGAAGAAGGAATACCA GCTTTCACTTGTGAAGAATATGTAAAGAGCCTAACTGCTAACCCCGAACTCATAGATCGT CTTGCTTGTTTGTCTGAAGAAGGGAATGAAATAGAAAGTGGAAAAATAATATTTTCAGAG CATCTTCCCTTAAGTAAGCTACAGCAAGGCATAAAATCTGGTACATACCTTCAAGGAACA TTTAGAGCTAGCAGGGAAAATTACTTGGAAGCTACAGTATGGATTCATGGCGACAATGAA GAAAATAAAGAGATAATCTTACAGGGACTTAAACATTTAAACAGAGCTGTTCACGAAGAT ATTGTGGCTGTGGAGCTTCTCCCCAAGAGTCAGTGGGTAGCACCATCTTCTGTGGTTTTA CATGATGAAGGTCAAAATGAAGAAGATGTGGAGAAAGAAGAAGAGACAGAACGAATGCTT AAGACTGCTGTAAGCGAGAAAATGTTGAAGCCTACAGGTAGAGTTGTAGGAATAATAAAA AGGAATTGGAGACCATATTGTGGCATGCTTTCCAAGTCTGACATTAAGGAGTCAAGAAGA CATCTCTTTACACCTGCTGATAAGAGAATCCCTCGAATTCGCATAGAAACCAGACAGGCT TCCACATTAGAAGGACGGAGAATTATTGTTGCTATTGATGGTTGGCCCAGAAATTCCAGA TATCCAAATGGACACTTTGTGAGAAATTTAGGTGATGTTGGAGAGAAAGAGACTGAAACA GAAGTTTTGTTACTTGAACACGATGTTCCCCATCAGCCTTTTTCACAGGCTGTTCTTAGT TTTCTGCCAAAGATGCCCTGGAGCATTACTGAAAAGGACATGAAAAACCGAGAAGACCTG AGGCATCTGTGTATTTGTAGTGTAGACCCACCAGGATGTACTGATATAGACGATGCTCTA CATTGTCGAGAACTCGAAAATGGAAATTTGGAGGTTGGTGTTCATATTGCTGATGTGAGC CATTTTATTAGGCCAGGAAATGCCTTGGATCAAGAATCAGCCAGAAGAGGAACAACTGTG TATCTTTGTGAAAAGAGGATTGACATGGTTCCAGAGTTGCTTAGCTCTAACTTGTGTTCC TTAAAATGTGACGTGGACAGGCTGGCATTTTCATGTATTTGGGAAATGAATCACAATGCT GAAATCTTAAAAACGAAGTTTACCAAAAGTGTTATTAATTCAAAGGCATCTCTGACGTAT GCTGAAGCTCAGTTGAGAATTGATTCAGCAAACATGAATGATGATATTACCACTAGTCTC CGTGGACTGAATAAACTAGCCAAAATTCTGAAGAAAAGAAGGATTGAAAAAGGGGCTTTG ACTCTATCCTCTCCTGAAGTTCGATTCCACATGGACAGTGAAACTCACGATCCTATAGAT CTGCAGACCAAGGAACTTAGGGAAACAAATTCCATGGTTGAAGAATTTATGTTACTTGCC AATATTTCTGTTGCAAAAAAAATTCATGAGGAATTTTCTGAACATGCTCTGCTTCGAAAA CATCCTGCTCCACCTCCATCAAATTATGAAATTCTTGTTAAGGCAGCCAGGTCAAGGAAT TTGGAAATTAAGACTGATACAGCCAAGTCTTTGGCTGAGTCTTTGGATCAGGCCGAATCT CCTACTTTTCCATATCTAAACACTCTGTTGAGAATATTAGCCACTCGCTGTATGATGCAA GCTGTGTACTTCTGTTCTGGAATGGATAATGATTTTCATCACTATGGCTTAGCGTCTCCA ATATACACACATTTTACTTCACCCATTAGAAGATACGCAGATGTCATTGTTCATCGGCTT TTGGCTGTGGCTATTGGGGCTGACTGTACTTATCCAGAGTTGACAGACAAACACAAGCTT GCAGATATATGTAAAAATCTAAATTTCCGGCACAAAATGGCTCAATATGCCCAACGTGCA TCAGTGGCTTTTCATACCCAGTTATTCTTCAAAAGCAAAGGAATAGTAAGTGAAGAAGCC TATATTTTATTTGTAAGAAAGAATGCCATTGTGGTATTAATTCCAAAGTATGGTTTAGAA GGGACAGTCTTTTTTGAAGAAAAGGACAAACCAAACCCACAGCTTATTTATGATGATGAG ATACCCTCACTTAAAATAGAAGATACAGTGTTCCATGTATTTGATAAAGTTAAAGTGAAA ATCATGTTAGACTCATCTAATCTTCAACATCAGAAGATCCGAATGTCCCTGGTAGAACCA CAGATACCAGGAATAAGCATTCCTACTGATACTTCAAACATGGACCTTAATGGACCAAAG AAAAAGAAGATGAAGCTTGGAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001128226 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001128226.1, NP_001121698.1 |
| RefSeq Size | 7577 bp |
| RefSeq ORF | 2787 bp |
| Locus ID | 22894 |
| UniProt ID | Q9Y2L1 |
| Protein Pathways | RNA degradation |
| Gene Summary | Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon and contains an alternate exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226264 | DIS3 (Myc-DDK-tagged)-Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 2 |
CNY 7,130.00 |
|
| RC226264L3 | Lenti-ORF clone of DIS3 (Myc-DDK-tagged)-Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 2 |
CNY 9,030.00 |
|
| RC226264L4 | Lenti-ORF clone of DIS3 (mGFP-tagged)-Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 2 |
CNY 9,030.00 |
|
| RG226264 | DIS3 (tGFP-tagged) - Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 2 |
CNY 7,890.00 |
