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P4HB (NM_000918) Human Untagged Clone
CAT＃: SC322507
P4HB (untagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB)
CNY 5,688.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLCRP1; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for SC322507
CCCGGCGGCGCCAACCGAAGCGCCCCGCCTGATCCGTGTCCGACATGCTGCGCCGCGCTC
TGCTGTGCCTGGCCGTGGCCGCCCTGGTGCGCGCCGACGCCCCCGAGGAGGAGGACCACG TCCTGGTGCTGCGGAAAAGCAACTTCGCGGAGGCGCTGGCGGCCCACAAGTACCTGCTGG TGGAGTTCTATGCCCCTTGGTGTGGCCACTGCAAGGCTCTGGCCCCTGAGTATGCCAAAG CCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATCAGGTTGGCCAAGGTGGACGCCACGG AGGAGTCTGACCTGGCCCAGCAGTACGGCGTGCGCGGCTATCCCACCATCAAGTTCTTCA GGAATGGAGACACGGCTTCCCCCAAGGAATATACAGCTGGCAGAGAGGCTGATGACATCG TGAACTGGCTGAAGAAGCGCACGGGCCCGGCTGCCACCACCCTGCCTGACGGCGCAGCTG CAGAGTCCTTGGTGGAGTCCAGCGAGGTGGCTGTCATCGGCTTCTTCAAGGACGTGGAGT CGGACTCTGCCAAGCAGTTTTTGCAGGCAGCAGAGGCCATCGATGACATACCATTTGGGA TCACTTCCAACAGTGACGTGTTCTCCAAATACCAGCTCGACAAAGATGGGGTTGTCCTCT TTAAGAAGTTTGATGAAGGCCGGAACAACTTTGAAGGGGAGGTCACCAAGGAGAACCTGC TGGACTTTATCAAACACAACCAGCTGCCCCTTGTCATCGAGTTCACCGAGCAGACAGCCC CGAAGATTTTTGGAGGTGAAATCAAGACTCACATCCTGCTGTTCTTGCCCAAGAGTGTGT CTGACTATGACGGCAAACTGAGCAACTTCAAAACAGCAGCCGAGAGCTTCAAGGGCAAGA TCCTGTTCATCTTCATCGACAGCGACCACACCGACAACCAGCGCATCCTCGAGTTCTTTG GCCTGAAGAAGGAAGAGTGCCCGGCCGTGCGCCTCATCACCCTGGAGGAGGAGATGACCA AGTACAAGCCCGAATCGGAGGAGCTGACGGCAGAGAGGATCACAGAGTTCTGCCACCGCT TCCTGGAGGGCAAAATCAAGCCCCACCTGATGAGCCAGGAGCTGCCGGAGGACTGGGACA AGCAGCCTGTCAAGGTGCTTGTTGGGAAGAACTTTGAAGACGTGGCTTTTGATGAGAAAA AAAACGTCTTTGTGGAGTTCTATGCCCCATGGTGTGGTCACTGCAAACAGTTGGCTCCCA TTTGGGATAAACTGGGAGAGACGTACAAGGACCATGAGAACATCGTCATCGCCAAGATGG ACTCGACTGCCAACGAGGTGGAGGCCGTCAAAGTGCACAGCTTCCCCACACTCAAGTTCT TTCCTGCCAGTGCCGACAGGACGGTCATTGATTACAACGGGGAACGCACGCTGGATGGTT TTAAGAAATTCCTGGAGAGCGGTGGCCAGGATGGGGCAGGGGATGATGACGATCTCGAGG ACCTGGAAGAAGCAGAGGAGCCAGACATGGAGGAAGACGATGATCAGAAAGCTGTGAAAG ATGAACTGTAATACGCAAAGCCAGACCCGGGCGCTGCCGAGACCCCTCGGGGGCTGCACA CCCAGCAGCAGCGCACGCCTCCGAAGCCTGCGGCCTCGCTTGAAGGAGGGCGTCGCCGGA AACCCAGGGAACCTCTCTGAAGTGACACCTCACCCCTACACACCGTCCGTTCACCCCCGT CTCTTCCTTCTGCTTTTCGGTTTTTGGAAAGGGATCCATCTCCAGGCAGCCCACCCTGGT GGGGCTTGTTTCCTGAAACCATGATGTACTTTTTCATACATGAGTCTGTCCAGAGTGCTT GCTACCGTGTTCGGAGTCTCGCTGCCTCCCTCCCGCGGGAGGTTTCTCCTCTTTTTGAAA ATTCCGTCTGTGGGATTTTTAGACATTTTTCGACATCAGGGTATTTGTTCCACCTTGGCC AGGCCTCCTCGGAGAAGCTTGTCCCCCGTGTGGGAGGGACGGAGCCGGACTGGACATGGT CACTCAGTACCGCCTGCAGTGTCGCCATGACTGATCATGGCTCTTGCATTTTTGGGTAAA TGGAGACTTCCGGATCCTGTCAGGGTGTCCCCCATGCCTGGAAGAGGAGCTGGTGGCTGC CAGCCCTGGGGCCCGGCACAGGCCTGGGCCTTCCCCTTCCCTCAAGCCAGGGCTCCTCCT CCTGTCGTGGGCTCATTGTGACCACTGGCCTCTCTACAGCACGGCCTGTGGCCTGTTCAA GGCAGAACCACGACCCTTGACTCCCGGGTGGGGAGGTGGCCAAGGATGCTGGAGCTGAAT CAGACGCTGACAGTTCTTCAGGCATTTCTATTTCACAATCGAATTGAACACATTGGCCAA ATAAAGTTGAAATTTTACCACCAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000918 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000918.3, NP_000909.2 |
| RefSeq Size | 2596 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 5034 |
| UniProt ID | P07237 |
| Domains | thiored |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202275 | P4HB (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB) |
CNY 5,672.00 |
|
| RC202275L1 | Lenti ORF clone of Human prolyl 4-hydroxylase, beta polypeptide (P4HB), Myc-DDK-tagged |
CNY 8,072.00 |
|
| RC202275L2 | Lenti ORF clone of Human prolyl 4-hydroxylase, beta polypeptide (P4HB), mGFP tagged |
CNY 5,890.00 |
|
| RC202275L3 | Lenti ORF clone of Human prolyl 4-hydroxylase, beta polypeptide (P4HB), Myc-DDK-tagged |
CNY 8,072.00 |
|
| RC202275L4 | Lenti ORF clone of Human prolyl 4-hydroxylase, beta polypeptide (P4HB), mGFP tagged |
CNY 8,072.00 |
|
| RG202275 | P4HB (tGFP-tagged) - Human prolyl 4-hydroxylase, beta polypeptide (P4HB) |
CNY 7,272.00 |
|
| SC119620 | P4HB (untagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB) |
CNY 5,688.00 |
