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P4HB (NM_000918) Human Untagged Clone
CAT＃: SC119620
P4HB (untagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB)
CNY 5,688.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLCRP1; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119620 sequence for NM_000918 edited (data generated by NextGen Sequencing)
ATGCTGCGCCGCGCTCTGCTGTGCCTGGCCGTGGCCGCCCTGGTGCGCGCCGACGCCCCC GAGGAGGAGGACCACGTCCTGGTGCTGCGGAAAAGCAACTTCGCGGAGGCGCTGGCGGCC CACAAGTACCTGCTGGTGGAGTTCTATGCCCCTTGGTGTGGCCACTGCAAGGCTCTGGCC CCTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATCAGGTTGGCC AAGGTGGACGCCACGGAGGAGTCTGACCTGGCCCAGCAGTACGGCGTGCGCGGCTATCCC ACCATCAAGTTCTTCAGGAATGGAGACACGGCTTCCCCCAAGGAATATACAGCTGGCAGA GAGGCTGATGACATCGTGAACTGGCTGAAGAAGCGCACGGGCCCGGCTGCCACCACCCTG CCTGACGGCGCAGCTGCAGAGTCCTTGGTGGAGTCCAGCGAGGTGGCTGTCATCGGCTTC TTCAAGGACGTGGAGTCGGACTCTGCCAAGCAGTTTTTGCAGGCAGCAGAGGCCATCGAT GACATACCATTTGGGATCACTTCCAACAGTGACGTGTTCTCCAAATACCAGCTCGACAAA GATGGGGTTGTCCTCTTTAAGAAGTTTGATGAAGGCCGGAACAACTTTGAAGGGGAGGTC ACCAAGGAGAACCTGCTGGACTTTATCAAACACAACCAGCTGCCCCTTGTCATCGAGTTC ACCGAGCAGACAGCCCCGAAGATTTTTGGAGGTGAAATCAAGACTCACATCCTGCTGTTC TTGCCCAAGAGTGTGTCTGACTATGACGGCAAACTGAGCAACTTCAAAACAGCAGCCGAG AGCTTCAAGGGCAAGATCCTGTTCATCTTCATCGACAGCGACCACACCGACAACCAGCGC ATCCTCGAGTTCTTTGGCCTGAAGAAGGAAGAGTGCCCGGCCGTGCGCCTCATCACCCTG GAGGAGGAGATGACCAAGTACAAGCCCGAATCGGAGGAGCTGACGGCAGAGAGGATCACA GAGTTCTGCCACCGCTTCCTGGAGGGCAAAATCAAGCCCCACCTGATGAGCCAGGAGCTG CCGGAGGACTGGGACAAGCAGCCTGTCAAGGTGCTTGTTGGGAAGAACTTTGAAGACGTG GCTTTTGATGAGAAAAAAAACGTCTTTGTGGAGTTCTATGCCCCATGGTGTGGTCACTGC AAACAGTTGGCTCCCATTTGGGATAAACTGGGAGAGACGTACAAGGACCATGAGAACATC GTCATCGCCAAGATGGACTCGACTGCCAACGAGGTGGAGGCCGTCAAAGTGCACAGCTTC CCCACACTCAAGTTCTTTCCTGCCAGTGCCGACAGGACGGTCATTGATTACAACGGGGAA CGCACGCTGGATGGTTTTAAGAAATTCCTGGAGAGCGGTGGCCAGGATGGGGCAGGGGAT GATGACGATCTCGAGGACCTGGAAGAAGCAGAGGAGCCAGACATGGAGGAAGACGATGAT CAGAAAGCTGTGAAAGATGAACTGTAA Clone variation with respect to NM_000918.3 >OriGene 5' read for NM_000918 unedited
GGCCGTTCCAAAATTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGCCGGG ACCGGCGCGCGCTCTCGTCGCCCCCGCTGTCCCGGCGGCGCCAACCGAAGCGCCCCGCCT GATCCGTGTCCGACATGCTGCGCCGCGCTCTGCTGTGCCTGGCCGTGGCCGCCCTGGTGC GCGCCGACGCCCCCGAGGAGGAGGACCACGTCCTGGTGCTGCGGAAAAGCAACTTCGCGG AGGCGCTGGCGGCCCACAAGTACCTGCTGGTGGAGTTCTATGCCCCTTGGTGTGGCCACT GCAAGGCTCTGGCCCCTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCG AGATCAGGTTGGCCAAGGTGGACGCCACGGAGGAGTCTGACCTGGCCCAGCAGTACGGCG TGCGCGGCTATCCCACCATCAAGTTCTTCAGGAATGGAGACACGGCTTCCCCCAAGGAAT ATACAGCTGGCAGAGAGGCTGATGACATCGTGAACTGGCTGAAGAAGCGCACGGGCCCGG CTGCCACCACCCTGCCTGACGGCGCAGCTGCAGAGTCCTTGGTGGAGTCCAGCGAGGTGG CTGTCATCGGCTTCTTCAAGGACGTGGAGTCGGACTCTGCCAAGCAGTTTTTGCAGGCAG CAGAGGCCATCGATGACATACCATTTGGGATCACTTCCAACAGTGACGTGTTCTCCAAAT ACCAGCTCGACAAGATGGNGTTGTCCTCTTTAAGAAGTTTGATGAAGGCCGGAACACTTT GAAGGGGAGGTCACCAAGNAGAACCTGCTGGACTTTATCAAACACAACCAGCTGCCCCTT GTCATCGAGTTCACCGAGCAGACAGCCCCCGAGATTTTTGGAGGTGAAATCAGACTCCAT CN >OriGene 3' read for NM_000918 unedited
TANNGATCGATTTTTTTTTTTTTTTTTTGGTGGTAAAATTTCAACTTTATTTGGCCAATG TGTTCAATTCGATTGTGAAATAGAAATGCCTGAAGAACTGTCAGCGTCTGATTCAGCTCC AGCATCCTTGGCCACCTCCCCACCCGGGAGTCAAGGGTCGTGGTTCTGCCTTGAACAGGC CACAGGCCGTGCTGTAGAGAGGCCAGTGGTCACAATGAGCCCACGACAGGAGGAGGAGCC CTGGCTTGAGGGAAGGGGAAGGCCCAGGCCTGTGCCGGGCCCCAGGGCTGGCAGCCACCA GCTCCTCTTCCAGGCATGGGGGACACCCTGACAGGATCCGGAAGTCTCCATTTACCCAAA AATGCAAGAGCCATGATCAGTCATGGCGACACTGCAGGCGGTACTGAGTGACCATGTCCA GTCCGGCTCCGTCCCTCCCACACGGGGGACAAGCTTCTTCGAGGAGGCCTGGCCAAGGTG GAACAAATACCCTGATGTCGAAAAATGTCTTAAAATCCCACAGACCGGATTTTCAAAAAG AGGAGAAACCTCCCGCGGGAAGGAGGCAGCGAGACTCCCAACACGGGAGCCAGCACTCTG GACAGACTCCTGTGTGAAAAAGTACATCATGGTTTTCAGGAAACAACCCCACCCAGGTGG GGCTGCCCTGAAAAGGATCCCTTTCCAAAAACCCCAAAACACAAAGGAAAAACCGGGGTG AACCGACCGTGTGTAGGGGGGAAGTGGTCCCTTCCAAGAAGTTCCCTGGGGTTTCCGGGG AACCACCTCTCTTTAACAGAGGCCCCAGGTCTTTGAGGGCTGCGCCTCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000918 |
| Insert Size | 2700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000918.2, NP_000909.2 |
| RefSeq Size | 2438 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 5034 |
| UniProt ID | P07237 |
| Domains | thiored |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Novel protein disulfide isomerase inhibitor with anti-cancer activity in multiple myeloma
,Vatolin, S;Phillips, JG;Jha, BK;Govindgari, S;Hu, JC;,
Cancer Research
,PubMed ID 27197150
[P4HB]
|
|
Pro-apoptotic activities of PDI and PDIA3: a role of Bcl-2 protein Bak
,Zhao, G;Lu, H;Li, C;,
J. Biol. Chem.
,PubMed ID 25697356
[P4HB]
|
|
Hyperoxia Resensitizes Chemoresistant Glioblastoma Cells to Temozolomide Through Unfolded Protein Response
,LEE, D;SUN, S;HO, AMYSW;KIANG, KMY;,
Anticancer Research
[P4HB]
|
|
Model-directed engineering of difficult-to-express monoclonal antibody production by Chinese hamster ovary cells
,Pybus, LP;Dean, G;West, NR;Smith, A;Daramola, O;Field, R;Wilkinson, SJ;James, DC;,
Biotechnol Bioeng. 2013 Sep 21
,PubMed ID 24081924
[P4HB]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202275 | P4HB (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB) |
CNY 5,672.00 |
|
| RC202275L1 | Lenti ORF clone of Human prolyl 4-hydroxylase, beta polypeptide (P4HB), Myc-DDK-tagged |
CNY 8,072.00 |
|
| RC202275L2 | Lenti ORF clone of Human prolyl 4-hydroxylase, beta polypeptide (P4HB), mGFP tagged |
CNY 5,890.00 |
|
| RC202275L3 | Lenti ORF clone of Human prolyl 4-hydroxylase, beta polypeptide (P4HB), Myc-DDK-tagged |
CNY 8,072.00 |
|
| RC202275L4 | Lenti ORF clone of Human prolyl 4-hydroxylase, beta polypeptide (P4HB), mGFP tagged |
CNY 8,072.00 |
|
| RG202275 | P4HB (tGFP-tagged) - Human prolyl 4-hydroxylase, beta polypeptide (P4HB) |
CNY 7,272.00 |
|
| SC322507 | P4HB (untagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB) |
CNY 5,688.00 |
