Calpain 10 (CAPN10) (NM_023083) Human Untagged Clone

CAT#: SC320709

CAPN10 (untagged)-Human calpain 10 (CAPN10), transcript variant 1



  "NM_023083" in other vectors (5)

CNY 4,928.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-CAPN10 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CANP10; NIDDM1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_023083.2 CACCTGCGGGGCCCTCGGGCTTGGAGGGCTGGGCCGGGCGGGGAACGGGCGGGGCGGGCC
GGAGGCGGCGGCGGCTGACTCGCCTTCTCTCCGGGGCTGCGACCCCGAGGCAACCGGCTG
CAGATGGGAGCCCGCGGAGCCGAGGATGCGGGCGGGCCGGGGCGCGACGCCGGCGAGGGA
GCTGTTCCGGGACGCCGCCTTCCCCGCCGCGGACTCCTCGCTCTTCTGCGACTTGTCTAC
GCCGCTGGCCCAGTTCCGCGAGGACATCACGTGGAGGCGGCCCCAGGAGATTTGTGCCAC
ACCCCGGCTGTTTCCAGATGACCCACGGGAAGGGCAGGTGAAGCAGGGGCTGCTGGGGGA
TTGCTGGTTCCTGTGTGCCTGCGCCGCGCTGCAGAAGAGCAGGCACCTCCTGGACCAGGT
CATTCCTCCGGGACAGCCGAGCTGGGCCGACCAGGAGTACCGGGGCTCCTTCACCTGTCG
CATTTGGCAGTTTGGACGCTGGGTGGAGGTGACCACAGATGACCGCCTGCCGTGCCTTGC
AGGGAGACTCTGTTTCTCCCGCTGCCAGAGGGAGGATGTGTTCTGGCTCCCCTTACTGGA
AAAGGTCTACGCCAAGGTCCATGGGTCCTACGAGCACCTGTGGGCCGGGCAGGTGGCGGA
TGCCCTGGTGGACCTGACCGGCGGCCTGGCAGAAAGATGGAACCTGAAGGGCGTAGCAGG
AAGCGGAGGCCAGCAGGACAGGCCAGGCCGCTGGGAGCACAGGACTTGTCGGCAGCTGCT
CCACCTGAAGGACCAGTGTCTGATCAGCTGCTGCGTGCTCAGCCCCAGAGCAGGTGCCCG
GGAGCTGGGGGAGTTCCATGCCTTCATTGTCTCGGACCTGCGGGAGCTCCAGGGTCAGGC
GGGCCAGTGCATCCTGCTGCTGCGGATCCAGAACCCCTGGGGCCGGCGGTGCTGGCAGGG
GCTCTGGAGAGAGGGGGGTGAAGGGTGGAGCCAGGTAGATGCAGCGGTAGCATCTGAGCT
CCTGTCCCAGCTCCAGGAAGGGGAGTTCTGGGTGGAGGAGGAGGAGTTCCTCAGGGAGTT
TGACGAGCTCACCGTTGGCTACCCGGTCACGGAGGCCGGCCACCTGCAGAGCCTCTACAC
AGAGAGGCTGCTCTGCCATACGCGGGCGCTGCCTGGGGCCTGGGTCAAGGGCCAGTCAGC
AGGAGGCTGCCGGAACAACAGCGGCTTTCCCAGCAACCCCAAATTCTGGCTGCGGGTCTC
AGAACCGAGTGAGGTGTACATTGCCGTCCTGCAGAGATCCAGGCTGCACGCGGCGGACTG
GGCAGGCCGGGCCCGGGCACTGGTGGGTGACAGTCATACTTCGTGGAGCCCAGCGAGCAT
CCCGGGCAAGCACTACCAGGCTGTGGGTCTGCACCTCTGGAAGGTAGAGAAGCGGCGGGT
CAATCTGCCTAGGGTCCTGTCCATGCCCCCCGTGGCTGGCACCGCGTGCCATGCATACGA
CCGGGAGGTCCACCTGCGTTGTGAGCTCTCACCGGGCTACTACCTGGCTGTCCCCAGCAC
CTTCCTGAAGGACGCGCCAGGGGAGTTCCTGCTCCGAGTCTTCTCTACCGGGCGAGTCTC
CCTTAGCGCCATCAGGGCAGTGGCCAAGAACACCACCCCCGGGGCAGCCCTGCCTGCGGG
GGAGTGGGGGACCGTGCAGCTACGGGGTTCTTGGAGAGTCGGCCAGACGGCGGGGGGCAG
CAGGAACTTTGCCTCATACCCCACCAACCCCTGCTTCCCCTTCTCGGTCCCCGAGGGCCC
TGGCCCCCGCTGCGTCCGCATCACTCTGCATCAGCACTGCCGGCCCAGTGACACCGAGTT
CCACCCCATCGGCTTCCATATCTTCCAGGTCCCAGAGGGTGGAAGGAGCCAGGACGCACC
CCCACTGCTGCTGCAGGAGCCGCTGCTGAGCTGCGTGCCACATCGCTACGCCCAGGAGGT
GAGCCGGCTCTGCCTCCTGCCTGCAGGCACCTACAAGGTTGTGCCCTCCACCTACCTGCC
GGACACAGAGGGGGCCTTCACAGTGACCATCGCAACCAGGATTGACAGGCCATCCATTCA
CAGCCAGGAGATGCTGGGCCAGTTCCTCCAAGAGGTCTCCGTCATGGCAGTGATGAAAAC
CTAACAGGGTGGCCCCCTGTGCCAGCTCAGGTGACTGGAGCCCGAGGGCCTGACAGGTTC
CCAGCAGCTGGGCCGGCCAGCCTTGCACTGTGGGGGCTGGTCCTGAGTCTTGGCCTGCCT
CCCAGCCCTGCCAGGGGGCTGCGGCCTAGGGGTCCACGGGAAGCCTCCGTCAGGAGAGAC
GCAGCCCTGGGGGCCAGCTGGTGCTGCAAGGAAGGGTGGGAAGCTTGCTGGCTTCTGTTG
CGCCACTGAGACGGCAGAGACCCCAGGATCCCAGAGCTTCCCAGGATCCCTCCCAGATCC
TCTGCTGACTCCATATGGAGGCCTCACACCCAGAGGGTAGGGCAGCAGATCTTCTTTATA
ACTATTTATTGTTCGAATCACTTTTAGGATGTAACTTTATAAATAAACATGAGCGCTGAT
GATTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_023083
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_023083.2, NP_075571.1
RefSeq Size 2626 bp
RefSeq ORF 2019 bp
Locus ID 11132
UniProt ID Q9HC96
Protein Families Druggable Genome, Protease
Gene Summary Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010]
Transcript Variant: This variant (1, also known as CAPN10a) represents the longer transcript and encodes the longer isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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