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Calpain 10 (CAPN10) (NM_023083) Human Untagged Clone
CAT＃: SC124129
CAPN10 (untagged)-Human calpain 10 (CAPN10), transcript variant 1
CNY 4,928.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CANP10; NIDDM1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124129 sequence for NM_023083 edited (data generated by NextGen Sequencing)
ATGCGGGCGGGCCGGGGCGCGACGCCGGCGAGGGAGCTGTTCCGGGACGCCGCCTTCCCC GCCGCGGACTCCTCGCTCTTCTGCGACTTGTCTACGCCGCTGGCCCAGTTCCGCGAGGAC ATCACGTGGAGGCGGCCCCAGGAGATTTGTGCCACACCCCGGCTGTTTCCAGATGACCCA CGGGAAGGGCAGGTGAAGCAGGGGCTGCTGGGGGATTGCTGGTTCCTGTGTGCCTGCGCC GCGCTGCAGAAGAGCAGGCACCTCCTGGACCAGGTCATTCCTCCGGGACAGCCGAGCTGG GCCGACCAGGAGTACCGGGGCTCCTTCACCTGTCGCATTTGGCAGTTTGGACGCTGGGTG GAGGTGACCACAGATGACCGCCTGCCGTGCCTTGCAGGGAGACTCTGTTTCTCCCGCTGC CAGAGGGAGGATGTGTTCTGGCTCCCCTTACTGGAAAAGGTCTACGCCAAGGTCCATGGG TCCTACGAGCACCTGTGGGCCGGGCAGGTGGCGGATGCCCTGGTGGACCTGACCGGCGGC CTGGCAGAAAGATGGAACCTGAAGGGCGTAGCAGGAAGCGGAGGCCAGCAGGACAGGCCA GGCCGCTGGGAGCACAGGACTTGTCGGCAGCTGCTCCACCTGAAGGACCAGTGTCTGATC AGCTGCTGCGTGCTCAGCCCCAGAGCAGGTGCCCGGGAGCTGGGGGAGTTCCATGCCTTC ATTGTCTCGGACCTGCGGGAGCTCCAGGGTCAGGCGGGCCAGTGCATCCTGCTGCTGCGG ATCCAGAACCCCTGGGGCCGGCGGTGCTGGCAGGGGCTCTGGAGAGAGGGGGGTGAAGGG TGGAGCCAGGTAGATGCAGCGGTAGCATCTGAGCTCCTGTCCCAGCTCCAGGAAGGGGAG TTCTGGGTGGAGGAGGAGGAGTTCCTCAGGGAGTTTGACGAGCTCACCGTTGGCTACCCG GTCACGGAGGCCGGCCACCTGCAGAGCCTCTACACAGAGAGGCTGCTCTGCCATACGCGG GCGCTGCCTGGGGCCTGGGTCAAGGGCCAGTCAGCAGGAGGCTGCCGGAACAACAGCGGC TTTCCCAGCAACCCCAAATTCTGGCTGCGGGTCTCAGAACCGAGTGAGGTGTACATTGCC GTCCTGCAGAGATCCAGGCTGCACGCGGCGGACTGGGCAGGCCGGGCCCGGGCACTGGTG GGTGACAGTCATACTTCGTGGAGCCCAGCGAGCATCCCGGGCAAGCACTACCAGGCTGTG GGTCTGCACCTCTGGAAGGTAGAGAAGCGGCGGGTCAATCTGCCTAGGGTCCTGTCCATG CCCCCCGTGGCTGGCACCGCGTGCCATGCATACGACCGGGAGGTCCACCTGCGTTGTGAG CTCTCACCGGGCTACTACCTGGCTGTCCCCAGCACCTTCCTGAAGGACGCGCCAGGGGAG TTCCTGCTCCGAGTCTTCTCTACCGGGCGAGTCTCCCTTAGCGCCATCAGGGCAGTGGCC AAGAACACCACCCCCGGGGCAGCCCTGCCTGCGGGGGAGTGGGGGACCGTGCAGCTACGG GGTTCTTGGAGAGTCGGCCAGACGGCGGGGGGCAGCAGGAACTTTGCCTCATACCCCACC AACCCCTGCTTCCCCTTCTCGGTCCCCGAGGGCCCTGGCCCCCGCTGCGTCCGCATCACT CTGCATCAGCACTGCCGGCCCAGTGACACCGAGTTCCACCCCATCGGCTTCCATATCTTC CAGGTCCCAGAGGGTGGAAGGAGCCAGGACGCACCCCCACTGCTGCTGCAGGAGCCGCTG CTGAGCTGCGTGCCACATCGCTACGCCCAGGAGGTGAGCCGGCTCTGCCTCCTGCCTGCA GGCACCTACAAGGTTGTGCCCTCCACCTACCTGCCGGACACAGAGGGGGCCTTCACAGTG ACCATCGCAACCAGGATTGACAGGCCATCCATTCACAGCCAGGAGATGCTGGGCCAGTTC CTCCAAGAGGTCTCCGTCATGGCAGTGATGAAAACCTAA Clone variation with respect to NM_023083.3 1860 g=>a >OriGene 5' read for NM_023083 unedited
GACCTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGGCCGGCGTACTGGCCTGGTC CAGCACCTGCGGGGCCCTCGGGCTTGGAGGGCTGGGCCGGGCGGGGAACGGGCGGGGCGG GCCGGAGGCGGCGGCGGCTGACTCGCCTTCTCTCCGGGGCTGCGACCCCGAGGCAACCGG CTGCAGATGGGAGCCCGCGGAGCCGAGGATGCGGGCGGGCCGGGGCGCGACGCCGGCGAG GGAGCTGTTCCGGGACGCCGCCTTCCCCGCCGCGGACTCCTCGCTCTTCTGCGACTTGTC TACGCCGCTGGCCCAGTTCCGCGAGGACATCACGTGGAGGCGGCCCCAGGAGATTTGTGC CACACCCCGGCTGTTTCCAGATGACCCACGGGAAAGGCAGGTGAAGCAGGGGCTGCTGGG GGATTGCTGGTTCCTGTGTGCCTGCGCCGCGCTGCAGAAGAGCAGGCACCTCCTGGACCA NGTCATTCCTCCGGGACAGCCGAGCTGGGCCGACCAGGAGTACCGGGGCTCCTTCACCTG TCGCATTTGGCAGTTGACGCTGGGTGGAGTGACCCAGATGACCGCTGCCGGCTTGAGGAG ACTTGTCTCCGTGCAGAGGAGAGGTCTGCTCCTTATGAAGGCTCCCAGTCATGTCACACA CTGTGCGGCAGTGCGTCCTGTGAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_023083 |
| Insert Size | 2700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_023083.1, NP_075571.1 |
| RefSeq Size | 2620 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 11132 |
| UniProt ID | Q9HC96 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010] Transcript Variant: This variant (1, also known as CAPN10a) represents the longer transcript and encodes the longer isoform (a). |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202556 | CAPN10 (Myc-DDK-tagged)-Human calpain 10 (CAPN10), transcript variant 1 |
CNY 4,920.00 |
|
| RC202556L3 | Lenti ORF clone of Human calpain 10 (CAPN10), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202556L4 | Lenti ORF clone of Human calpain 10 (CAPN10), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202556 | CAPN10 (tGFP-tagged) - Human calpain 10 (CAPN10), transcript variant 1 |
CNY 6,520.00 |
|
| SC320709 | CAPN10 (untagged)-Human calpain 10 (CAPN10), transcript variant 1 |
CNY 4,928.00 |
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