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ALAS1 (NM_199166) Human Untagged Clone
CAT＃: SC320404
ALAS1 (untagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2
CNY 4,776.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALAS; ALAS-H; ALAS3; ALASH; MIG4 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_199166.1
AGCGTTTCGTTTGGACTTCTCGACTTGAGTGCCCGCCTCCTTCGCCGCCGCCTCTGCAGT
CCTCAGCGCAGTTATGCCCAGTTCTTCCCGCTGTGGGGACACGACCACGGAGGAATCCTT GCTTCAGGGACTCGGGACCCTGCTGGACCCCTTCCTCGGGTTTAGGGGATGTGGGGACCA GGAGAAAGTCAGGATCCCTAAGAGTCTTCCCTGCCTGGATGGATGAGTGGCTTCTTCTCC ACCTAGATTCTTTCCACAGGAGCCAGCATACTTCCTGAACATGGAGAGTGTTGTTCGCCG CTGCCCATTCTTATCCCGAGTCCCCCAGGCCTTTCTGCAGAAAGCAGGCAAATCTCTGTT GTTCTATGCCCAAAACTGCCCCAAGATGATGGAAGTTGGGGCCAAGCCAGCCCCTCGGGC ATTGTCCACTGCAGCAGTACACTACCAACAGATCAAAGAAACCCCTCCGGCCAGTGAGAA AGACAAAACTGCTAAGGCCAAGGTCCAACAGACTCCTGATGGATCCCAGCAGAGTCCAGA TGGCACACAGCTTCCGTCTGGACACCCCTTGCCTGCCACAAGCCAGGGCACTGCAAGCAA ATGCCCTTTCCTGGCAGCACAGATGAATCAGAGAGGCAGCAGTGTCTTCTGCAAAGCCAG TCTTGAGCTTCAGGAGGATGTGCAGGAAATGAATGCCGTGAGGAAAGAGGTTGCTGAAAC CTCAGCAGGCCCCAGTGTGGTTAGTGTGAAAACCGATGGAGGGGATCCCAGTGGACTGCT GAAGAACTTCCAGGACATTATGCAAAAGCAAAGACCAGAAAGAGTGTCTCATCTTCTTCA AGATAACTTGCCAAAATCTGTTTCCACTTTTCAGTATGATCGTTTCTTTGAGAAAAAAAT TGATGAGAAAAAGAATGACCACACCTATCGAGTTTTTAAAACTGTGAACCGGCGAGCACA CATCTTCCCCATGGCAGATGACTATTCAGACTCCCTCATCACCAAAAAGCAAGTGTCAGT CTGGTGCAGTAATGACTACCTAGGAATGAGTCGCCACCCACGGGTGTGTGGGGCAGTTAT GGACACTTTGAAACAACATGGTGCTGGGGCAGGTGGTACTAGAAATATTTCTGGAACTAG TAAATTCCATGTGGACTTAGAGCGGGAGCTGGCAGACCTCCATGGGAAAGATGCCGCACT CTTGTTTTCCTCGTGCTTTGTGGCCAATGACTCAACCCTCTTCACCCTGGCTAAGATGAT GCCAGGCTGTGAGATTTACTCTGATTCTGGGAACCATGCCTCCATGATCCAAGGGATTCG AAACAGCCGAGTGCCAAAGTACATCTTCCGCCACAATGATGTCAGCCACCTCAGAGAACT GCTGCAAAGATCTGACCCCTCAGTCCCCAAGATTGTGGCATTTGAAACTGTCCATTCAAT GGATGGGGCGGTGTGCCCACTGGAAGAGCTGTGTGATGTGGCCCATGAGTTTGGAGCAAT CACCTTCGTGGATGAGGTCCACGCAGTGGGGCTTTATGGGGCTCGAGGCGGAGGGATTGG GGATCGGGATGGAGTCATGCCAAAAATGGACATCATTTCTGGAACACTTGGCAAAGCCTT TGGTTGTGTTGGAGGGTACATCGCCAGCACGAGTTCTCTGATTGACACCGTACGGTCCTA TGCTGCTGGCTTCATCTTCACCACCTCTCTGCCACCCATGCTGCTGGCTGGAGCCCTGGA GTCTGTGCGGATCCTGAAGAGCGCTGAGGGACGGGTGCTTCGCCGCCAGCACCAGCGCAA CGTCAAACTCATGAGACAGATGCTAATGGATGCCGGCCTCCCTGTTGTCCACTGCCCCAG CCACATCATCCCTGTGCGGGTTGCAGATGCTGCTAAAAACACAGAAGTCTGTGATGAACT AATGAGCAGACATAACATCTACGTGCAAGCAATCAATTACCCTACGGTGCCCCGGGGAGA AGAGCTCCTACGGATTGCCCCCACCCCTCACCACACACCCCAGATGATGAACTACTTCCT TGAGAATCTGCTAGTCACATGGAAGCAAGTGGGGCTGGAACTGAAGCCTCATTCCTCAGC TGAGTGCAACTTCTGCAGGAGGCCACTGCATTTTGAAGTGATGAGTGAAAGAGAGAAGTC CTATTTCTCAGGCTTGAGCAAGTTGGTATCTGCTCAGGCCTGAGCATGACCTCAATTATT TCACTTAACCCCAGGCCATTATCATATCCAGATGGTCTTCAGAGTTGTCTTTATATGTGA ATTAAGTTATATTAAATTTTAATCTATAGTAAAAACATAGTCCTGGAAATAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_199166 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_199166.1, NP_954635.1 |
| RefSeq Size | 2258 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 211 |
| UniProt ID | P13196 |
| Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism |
| Gene Summary | This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015] Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203996 | ALAS1 (Myc-DDK-tagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2 |
CNY 4,768.00 |
|
| RC203996L3 | Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203996L4 | Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG203996 | ALAS1 (tGFP-tagged) - Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2 |
CNY 6,368.00 |
|
| SC127743 | ALAS1 (untagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2 |
CNY 4,776.00 |
