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ALAS1 (NM_199166) Human Untagged Clone
CAT＃: SC127743
ALAS1 (untagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2
CNY 4,776.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALAS; ALAS-H; ALAS3; ALASH; MIG4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_199166, the custom clone sequence may differ by one or more nucleotides
ATGGAGAGTGTTGTTCGCCGCTGCCCATTCTTATCCCGAGTCCCCCAGGCCTTTCTGCAGAAAGCAGGCA AATCTCTGTTGTTCTATGCCCAAAACTGCCCCAAGATGATGGAAGTTGGGGCCAAGCCAGCCCCTCGGGC ATTGTCCACTGCAGCAGTACACTACCAACAGATCAAAGAAACCCCTCCGGCCAGTGAGAAAGACAAAACT GCTAAGGCCAAGGTCCAACAGACTCCTGATGGATCCCAGCAGAGTCCAGATGGCACACAGCTTCCGTCTG GACACCCCTTGCCTGCCACAAGCCAGGGCACTGCAAGCAAATGCCCTTTCCTGGCAGCACAGATGAATCA GAGAGGCAGCAGTGTCTTCTGCAAAGCCAGTCTTGAGCTTCAGGAGGATGTGCAGGAAATGAATGCCGTG AGGAAAGAGGTTGCTGAAACCTCAGCAGGCCCCAGTGTGGTTAGTGTGAAAACCGATGGAGGGGATCCCA GTGGACTGCTGAAGAACTTCCAGGACATCATGCAAAAGCAAAGACCAGAAAGAGTGTCTCATCTTCTTCA AGATAACTTGCCAAAATCTGTTTCCACTTTTCAGTATGATCGTTTCTTTGAGAAAAAAATTGATGAGAAA AAGAATGACCACACCTATCGAGTTTTTAAAACTGTGAACCGGCGAGCACACATCTTCCCCATGGCAGATG ACTATTCAGACTCCCTCATCACCAAAAAGCAAGTGTCAGTCTGGTGCAGTAATGACTACCTAGGAATGAG TCGCCACCCACGGGTGTGTGGGGCAGTTATGGACACTTTGAAACAACATGGTGCTGGGGCAGGTGGTACT AGAAATATTTCTGGAACTAGTAAATTCCATGTGGACTTAGAGCGGGAGCTGGCAGACCTCCATGGGAAAG ATGCCGCACTCTTGTTTTCCTCGTGCTTTGTGGCCAATGACTCAACCCTCTTCACCCTGGCTAAGATGAT GCCAGGCTGTGAGATTTACTCTGATTCTGGGAACCATGCCTCCATGATCCAAGGGATTCGAAACAGCCGA GTGCCAAAGTACATCTTCCGCCACAATGATGTCAGCCACCTCAGAGAACTGCTGCAAAGATCTGACCCCT CAGTCCCCAAGATTGTGGCATTTGAAACTGTCCATTCAATGGATGGGGCGGTGTGCCCACTGGAAGAGCT GTGTGATGTGGCCCATGAGTTTGGAGCAATCACCTTCGTGGATGAGGTCCACGCAGTGGGGCTTTATGGG GCTCGAGGCGGAGGGATTGGGGATCGGGATGGAGTCATGCCAAAAATGGACATCATTTCTGGAACACTTG GCAAAGCCTTTGGTTGTGTTGGAGGGTACATCGCCAGCACGAGTTCTCTGATTGACACCGTACGGTCCTA TGCTGCTGGCTTCATCTTCACCACCTCTCTGCCACCCATGCTGCTGGCTGGAGCCCTGGAGTCTGTGCGG ATCCTGAAGAGCGCTGAGGGACGGGTGCTTCGCCGCCAGCACCAGCGCAACGTCAAACTCATGAGACAGA TGCTAATGGATGCCGGCCTCCCTGTTGTCCACTGCCCCAGCCACATCATCCCTGTGCGGGTTGCAGATGC TGCTAAAAACACAGAAGTCTGTGATGAACTAATGAGCAGACATAACATCTACGTGCAAGCAATCAATTAC CCTACGGTGCCCCGGGGAGAAGAGCTCCTACGGATTGCCCCCACCCCTCACCACACACCCCAGATGATGA ACTACTTCCTTGAGAATCTGCTAGTCACATGGAAGCAAGTGGGGCTGGAACTGAAGCCTCATTCCTCAGC TGAGTGCAACTTCTGCAGGAGGCCACTGCATTTTGAAGTGATGAGTGAAAGAGAGAAGTCCTATTTCTCA GGCTTGAGCAAGTTGGTATCTGCTCAGGCCTGA >OriGene 5' read for NM_199166 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGCAACGAGCGT TTCGTTTGGACTTCTCGACTTGAGTGCCCGCCTCCTTCGCCGCCGCCTCTGCAGTCCTCA GCGCAGTCTTTCCACAGGAGCCAGCATACTTCCTGAACATGGAGAGTGTTGTTCGCCGCT GCCCATTCTTATCCCGAGTCCCCCAGGCCTTTCTGCAGAAAGCAGGCAAATCTCTGTTGT TCTATGCCCAAAACTGCCCCAAGATGATGGAAGTTGGGGCCAAGCCAGCCCCTCGGGCAT TGTCCACTGCAGCAGTACACTACCAACAGATCAAAGAAACCCCTCCGGCCAGTGAGAAAG ACAAAACTGCTAAGGCCAAGGTCCAACAGACTCCTGATGGATCCCAGCAGAGTCCAGATG GCACACAGCTTCCGTCTGGACACCCCTTGCCTGCCACAAGCCAGGGCACTGCAAGCAAAT GCCCTTTCCTGGCAGCACAGATGAATCAGAGAGGCAGCAGTGTCTTCTGCAAAGCCAGTC TTGAGCTTCAGGAGGATGTGCAGGAAATGAATGCCGTGAGGAAAGAGGTTGCTGAAACCT CAGCAGGCCCCAGTGTGGTTAGTGTGAAAACCGATGGAGGGGATCCCAGTGGACTGCTGA AGAACTTCCAGGACATTATGCAAAAGCAAAGACCAGANAGAGTGTCTCATCTTCTTCAAG ATAACTTGCCAAAATCTGTTTCCACTNTTCAGTATGATCGTTTCTTTGAGAAAAAATTGA TGAGAAAAAGATGACCCACCTATCGAGTTTTTAAACTGTGAACCGCGAGCACACATCTCC CATGGCAGAGACTATTCAGACTCCTCTCACANAAGCAGTGTCAGTCTGGTGCAGTATGAC TCCTAGGATGAGC >OriGene 3' read for NM_199166 unedited
CCGTGAGGTCGAGTAAAAATCCTTTTTTTTTTTTTTTTTACTATAGATTAAAATTTAATA TAACTTAATTCACATATAAAGACAACTCTGAAGACCATCTGGATATGATAATGGCCTGGG GTTAAGTGAAATAATTGAGGCCATGCTCAGGCCTGAGCAGATACCAACTTGCTCAAGCCT GAGAAATAGGACTTCTTTTTTTCACTCATCACTTCAAAATGCAGTGGCCTCCTGCAGAAG TTGCACTCAGCTGAGGAATGAGGCTTCAGTCCCAGCCCCACTTGCTTCCATGTGACTAGC AGATTCTCAAGGAAGTAGCCCATCACTCGGGGTGTGTGGTGAGGGGTGGGGGCAATCCGT AGGAGCTCTTCTCCCCGGGGCACCGCAGGGTAATTGATTGCTCGCACGTACATGTTATGA CCGCTCATTAGCCCATCACAGACTTCTGCGCCTTTTACCAACACCTGCAACCCGCACAGG GATGATGTGGCTGGGGCAGTGGACAACAGGGAGGGCGGCATCCATTACCATCTGCCCCAT GAGTTTGACGCTGCGCTGGTGCCTGCGGCGAAACACCCGTACCTAAACGCTCTTCAGGAT CCGCACAGACTATAGGGCTCCCCCCCGCACCCCGGGCGGCAGACAGGTGGTGAAAATGAA CCCAACCCCTAACCGCCCGCCCGGCGCAAAACTACATAAATCGGGCTCGGCGAAGAACCC TCTCAAAAAACCCAAAAGGCTCTGCCAAGGGGTATCCAAACCGAGCACCATTTTTGCCCG AGCCCCCCCCGAACCCACAATGCCTCCGACTTGAACCCCATAAAGCCCCACTGGCGGGTA CTCATACACCAAGGGCACGCCCCAAACTATGGGCCCCCTACCAATCTTCTCACGGGTCAC CCGCCCTTCCTTGACGGAATGCCCACAGCCCAAATCTGGAACCGACGGGAAAACCCTTAC CATTCCCTGAGGGGTGACCCCTTGGGCGAAAACGCCTCGCCTCCGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_199166 |
| Insert Size | 2260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_199166.1, NP_954635.1 |
| RefSeq Size | 2258 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 211 |
| UniProt ID | P13196 |
| Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism |
| Gene Summary | This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015] Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203996 | ALAS1 (Myc-DDK-tagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2 |
CNY 4,768.00 |
|
| RC203996L3 | Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203996L4 | Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG203996 | ALAS1 (tGFP-tagged) - Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2 |
CNY 6,368.00 |
|
| SC320404 | ALAS1 (untagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2 |
CNY 4,776.00 |
