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SMAD6 (NM_005585) Human Untagged Clone
CAT＃: SC320357
SMAD6 (untagged)-Human SMAD family member 6 (SMAD6), transcript variant 1
CNY 10,568.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AOVD2; HsT17432; MADH6; MADH7 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_005585 edited
CCCCGGTAACCGGAGACCGCCTCCCCCCCACCCCTGGCGCCAAAGGATATCGTATGTTCA GGTCCAAACGCTCGGGGCTGGTGCGGCGACTTTGGCGAAGTCGTGTGGTCCCCGACCGGG AGGAAGGCGGCAGCGGCGGCGGCGGTGGCGGCGACGAGGATGGGAGCTTGGGCAGCCGAG CTGAGCCGGCCCCGCGGGCAAGAGAGGGCGGAGGCTGCGGCCGCTCCGAAGTCCGCCCGG TAGCCCCGCGGCGGCCCCGGGACGCAGTGGGACAGCGAGGCGCCCAGGGCGCGGGGAGGC GCCGGCGCGCAGGGGGCCCCCCGAGGCCCATGTCGGAGCCAGGGGCCGGCGCTGGGAGCT CCCTGCTGGACGTGGCGGAGCCGGGAGGCCCGGGCTGGCTGCCCGAGAGTGACTGCGAGA CGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCGCCGGCGCGCCCCGGGACGCCAGCG ACCCCCTGGCCGGGGCGGCCCTGGAGCCGGCGGGCGGCGGGCGGAGTCGCGAAGCGCGCT CGCGGCTGCTGCTGCTGGAGCAGGAACTCAAAACCGTCACGTACTCGCTGCTGAAGCGGC TCAAGGAGCGCTCGCTGGACACGCTGCTGGAGGCGGTGGAGTCCCGCGGCGGCGTGCCGG GCGGCTGCGTGCTGGTGCCGCGCGCCGACCTCCGCCTGGGCGGCCAGCCCGCGCCGCCGC AGCTGCTGCTCGGCCGCCTCTTTCGCTGGCCCGACCTGCAGCACGCCGTGGAGCTGAAGC CCCTGTGCGGCTGCCACAGCTTCGCCGCCGCCGCCGACGGCCCTACCGTGTGCTGCAACC CCTACCACTTCAGCCGGCTCTGCGGGCCCGAATCTCCGCCACCTCCCTACTCTCGGCTGT CTCCTCGCGACGAGTACAAGCCACTGGATCTGTCCGATTCCACATTGTCTTACACTGAAA CGGAGGCTACCAACTCCCTCATCACTGCTCCGGGTGAATTCTCAGACGCCAGCATGTCTC CGGACGCCACCAAGCCGAGCCACTGGTGCAGCGTGGCGTACTGGGAGCACCGGACGCGCG TGGGCCGCCTCTATGCGGTGTACGACCAGGCCGTCAGCATCTTCTACGACCTACCTCAGG GCAGCGGCTTCTGCCTGGGCCAGCTCAACCTGGAGCAGCGCAGCGAGTCGGTGCGGCGAA CGCGCAGCAAGATCGGCTTCGGCATCCTGCTCAGCAAGGAGCCCGACGGCGTGTGGGCCT ACAACCGCGGCGAGCACCCCATCTTCGTCAACTCCCCGACGCTGGACGCGCCCGGCGGCC GCGCCCTGGTCGTGCGCAAGGTGCCCCCCGGCTACTCCATCAAGGTGTTCGACTTCGAGC GCTCGGGCCTGCAGCACGCGCCCGAGCCCGACGCCGCCGACGGCCCCTACGACCCCAACA GCGTCCGCATCAGCTTCGCCAAGGGCTGGGGGCCCTGCTACTCCCGGCAGTTCATCACCT CCTGCCCCTGCTGGCTGGAGATCCTCCTCAACAACCCCAGATAGTGGCGGCCCCGGCGGG AGGGGCGGGTGGGAGGCCGCGGCCACCGCCACCTGCCGGCCTCGAGAGGGGCCGATGCCC AGAGACACAGCCCCCACGGACAAAACCCCCCAGATATCATCTACCTAGATTTAATATAAA GTTTTATATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_005585 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005585.3, NP_005576.3 |
| RefSeq Size | 2912 bp |
| RefSeq ORF | 2886 bp |
| Locus ID | 4091 |
| UniProt ID | O43541 |
| Domains | DWB, DWA, MH1 |
| Protein Families | Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| Protein Pathways | TGF-beta signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014] Transcript Variant: This variant (1) encodes the supported protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203603 | SMAD6 (Myc-DDK-tagged)-Human SMAD family member 6 (SMAD6), transcript variant 1 |
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| RC203603L3 | Lenti-ORF clone of SMAD6 (Myc-DDK-tagged)-Human SMAD family member 6 (SMAD6), transcript variant 1 |
CNY 5,890.00 |
|
| RC203603L4 | Lenti-ORF clone of SMAD6 (mGFP-tagged)-Human SMAD family member 6 (SMAD6), transcript variant 1 |
CNY 5,890.00 |
|
| RG203603 | SMAD6 (tGFP-tagged) - Human SMAD family member 6 (SMAD6), transcript variant 1 |
CNY 7,088.00 |
|
| SC127117 | SMAD6 (untagged)-Human SMAD family member 6 (SMAD6), transcript variant 1 |
CNY 5,130.00 |
