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ZNRF3 (NM_032173) Human Untagged Clone
CAT＃: SC318318
ZNRF3 (untagged)-Human zinc and ring finger 3 (ZNRF3), transcript variant 2
CNY 16,250.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BK747E2.3; RNF203 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318318 representing NM_032173.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCACCCACTGGGCCTATGTAATAACAATGACGAAGAGGACTTGTATGAATATGGCTGGGTAGGAGTG GTGAAGCTGGAACAGCCAGAATTGGACCCGAAACCATGCCTCACTGTCCTAGGCAAGGCCAAGCGAGCA GTACAGCGGGGAGCTACTGCAGTCATCTTTGATGTGTCTGAAAACCCAGAAGCTATTGATCAGCTGAAC CAGGGCTCTGAAGACCCGCTCAAGAGGCCGGTGGTGTATGTGAAGGGTGCAGATGCCATTAAGCTGATG AACATCGTCAACAAGCAGAAAGTGGCTCGAGCAAGGATCCAGCACCGCCCTCCTCGACAACCCACTGAA TACTTTGACATGGGGATTTTCCTGGCTTTCTTCGTCGTGGTCTCCTTGGTCTGCCTCATCCTCCTTGTC AAAATCAAGCTGAAGCAGCGACGCAGTCAGAATTCCATGAACAGGCTGGCTGTGCAGGCTCTAGAGAAG ATGGAAACCAGAAAGTTCAACTCCAAGAGCAAGGGGCGCCGGGAGGGGAGCTGTGGGGCCCTGGACACA CTCAGCAGCAGCTCCACGTCCGACTGTGCCATCTGTCTGGAGAAGTACATTGATGGAGAGGAGCTGCGG GTCATCCCCTGTACTCACCGGTTTCACAGGAAGTGCGTGGACCCCTGGCTGCTGCAGCACCACACCTGC CCCCACTGTCGGCACAACATCATAGAACAAAAGGGAAACCCAAGCGCGGTGTGTGTGGAGACCAGCAAC CTCTCACGTGGTCGGCAGCAGAGGGTGACCCTGCCGGTGCATTACCCCGGCCGCGTGCACAGGACCAAC GCCATCCCAGCCTACCCTACGAGGACAAGCATGGACTCCCACGGCAACCCCGTCACCTTGCTGACCATG GACCGGCACGGGGAGCAGAGCCTCTATTCCCCGCAGACCCCCGCCTACATCCGCAGCTACCCACCCCTC CACCTGGACCACAGCCTGGCCGCTCACCGCTGCGGCCTGGAGCACCGGGCCTACTCCCCAGCCCACCCC TTCCGCAGGCCCAAGTTGAGTGGCCGCAGCTTCTCCAAGGCAGCTTGCTTCTCCCAGTATGAGACCATG TACCAGCACTACTACTTCCAGGGCCTCAGCTACCCGGAGCAGGAGGGGCAGTCCCCACCTAGCCTCGCA CCCCGGGGCCCGGCCCGTGCCTTTCCTCCGAGCGGCAGTGGCAGCCTGCTCTTCCCCACCGTGGTGCAC GTGGCCCCGCCCTCCCACCTGGAGAGCGGCAGCACGTCCAGCTTCAGCTGCTATCACGGCCACCGCTCG GTGTGCAGTGGCTACCTGGCCGACTGCCCAGGCAGCGACAGCAGCAGCAGCAGCAGCTCCGGCCAGTGC CACTGTTCCTCCAGTGACTCTGTGGTAGACTGCACTGAGGTCAGCAACCAGGGCGTGTACGGGAGCTGC TCCACCTTCCGCAGCTCCCTCAGCAGCGACTATGACCCCTTCATCTACCGCAGCCGGAGCCCCTGTCGT GCCAGTGAGGCGGGGGGCTCGGGCAGCTCGGGCCGGGGACCTGCCCTGTGCTTCGAGGGCTCCCCGCCT CCCGAGGAGCTCCCGGCGGTGCACAGTCATGGTGCTGGGCGGGGCGAGCCTTGGCCGGGCCCTGCCTCT CCCTCGGGGGATCAGGTGTCCACCTGCAGCCTGGAGATGAACTACAGCAGCAACTCCTCCCTGGAGCAC AGGGGGCCCAATAGCTCTACCTCAGAAGTGGGGCTCGAGGCTTCTCCTGGGGCCGCCCCTGACCTCAGG AGGACCTGGAAGGGGGGCCACGAGTTGCCGTCGTGTGCCTGCTGCTGCGAGCCCCAGCCCTCCCCAGCC GGGCCTAGCGCCGGAGCAGCTGGCAGCAGCACCTTGTTCCTGGGGCCCCACCTCTACGAGGGCTCTGGC CCGGCGGGTGGGGAGCCCCAGTCAGGAAGCTCCCAGGGCTTGTACGGCCTTCACCCCGACCATTTGCCC AGGACAGATGGGGTGAAATACGAGGGTCTGCCCTGCTGCTTCTATGAAGAGAAGCAGGTGGCCCGCGGG GGCGGAGGGGGCAGCGGCTGCTACACTGAGGACTACTCGGTGAGTGTGCAGTACACGCTCACCGAGGAA CCACCGCCCGGCTGCTACCCCGGGGCCCGGGACCTGAGCCAGCGCATCCCCATCATTCCAGAGGATGTG GACTGTGATCTGGGCCTGCCCTCGGACTGCCAAGGGACCCACAGCCTCGGCTCCTGGGGTGGGACGCGA GGCCCGGATACCCCACGGCCCCACAGGGGCCTGGGAGCAACCCGGGAAGAGGAGCGGGCTCTGTGCTGC CAGGCTAGGGCCCTACTGCGGCCTGGCTGCCCTCCGGAGGAGGCGGGTGCTGTCAGGGCCAACTTCCCT AGTGCCCTCCAGGACACTCAGGAGTCCAGCACCACTGCCACTGAGGCTGCAGGACCGAGATCTCACTCA GCAGACAGCAGCAGCCCGGGAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_032173 |
| Insert Size | 2511 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032173.3 |
| RefSeq Size | 6489 bp |
| RefSeq ORF | 2511 bp |
| Locus ID | 84133 |
| UniProt ID | Q9ULT6 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 89.9 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone (PubMed:22575959). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219546 | ZNRF3 (Myc-DDK-tagged)-Human zinc and ring finger 3 (ZNRF3), transcript variant 2 |
CNY 6,120.00 |
|
| RC219546L1 | Lenti ORF clone of Human zinc and ring finger 3 (ZNRF3), transcript variant 2, Myc-DDK-tagged |
CNY 8,520.00 |
|
| RC219546L2 | Lenti ORF clone of Human zinc and ring finger 3 (ZNRF3), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RC219546L3 | Lenti ORF clone of Human zinc and ring finger 3 (ZNRF3), transcript variant 2, Myc-DDK-tagged |
CNY 8,520.00 |
|
| RC219546L4 | Lenti ORF clone of Human zinc and ring finger 3 (ZNRF3), transcript variant 2, mGFP tagged |
CNY 8,520.00 |
|
| RG219546 | ZNRF3 (tGFP-tagged) - Human zinc and ring finger 3 (ZNRF3), transcript variant 2 |
CNY 7,720.00 |
|
| SC106136 | ZNRF3 (untagged)-Human zinc and ring finger 3 (ZNRF3), transcript variant 2 |
CNY 1,200.00 |
