PAPP A2 (PAPPA2) (NM_021936) Human Untagged Clone
CAT#: SC318308
PAPPA2 (untagged)-Human pappalysin 2 (PAPPA2), transcript variant 2
CNY 15,870.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | PAPP-A2; PAPP-E; PAPPE; PLAC3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC318308 representing NM_021936.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGTGCTTAAAGATCCTAAGAATAAGCCTGGCGATTTTGGCTGGGTGGGCACTCTGTTCTGCCAAC TCTGAGCTGGGCTGGACACGCAAGAAATCCTTGGTTGAGAGGGAACACCTGAATCAGGTGCTGTTGGAA GGAGAACGTTGTTGGCTGGGGGCCAAGGTTCGAAGACCCAGAGCTTCTCCACAGCATCACCTCTTTGGA GTCTACCCCAGCAGGGCTGGGAACTACCTAAGGCCCTACCCCGTGGGGGAGCAAGAAATCCATCATACA GGACGCAGCAAACCAGACACTGAAGGAAATGCTGTGAGCCTTGTTCCCCCAGACCTGACTGAAAATCCA GCAGGACTGAGGGGTGCAGTTGAAGAGCCGGCTGCCCCATGGGTAGGGGATAGTCCTATTGGGCAATCT GAGCTGCTGGGAGATGATGACGCTTATCTCGGCAATCAAAGATCCAAGGAGTCTCTAGGTGAGGCCGGG ATTCAGAAAGGCTCAGCCATGGCTGCCACTACTACCACCGCCATTTTCACAACCCTGAACGAACCCAAA CCAGAGACCCAAAGGAGGGGCTGGGCCAAGTCCAGGCAGCGTCGCCAAGTGTGGAAGAGGCGGGCGGAA GATGGGCAGGGAGACTCCGGTATCTCTTCACATTTCCAACCTTGGCCCAAGCATTCCCTTAAACACAGG GTCAAAAAGAGTCCACCGGAGGAAAGCAACCAAAATGGTGGAGAGGGCTCCTACCGAGAAGCAGAGACC TTTAACTCCCAAGTAGGACTGCCCATCTTATACTTCTCTGGGAGGCGGGAGCGGCTGCTGCTGCGTCCA GAAGTGCTGGCTGAGATTCCCCGGGAGGCGTTCACAGTGGAAGCCTGGGTTAAACCGGAGGGAGGACAG AACAACCCAGCCATCATCGCAGGTGTGTTTGATAACTGCTCCCACACTGTCAGTGACAAAGGCTGGGCC CTGGGGATCCGCTCAGGGAAGGACAAGGGAAAGCGGGATGCTCGCTTCTTCTTCTCCCTCTGCACCGAC CGCGTGAAGAAAGCCACCATCTTGATTAGCCACAGTCGCTACCAACCAGGCACATGGACCCATGTGGCA GCCACTTACGATGGACGGCACATGGCCCTGTATGTGGATGGCACTCAGGTGGCTAGCAGTCTAGACCAG TCTGGTCCCCTGAACAGCCCCTTCATGGCATCTTGCCGCTCTTTGCTCCTGGGGGGAGACAGCTCTGAG GATGGGCACTATTTCCGTGGACACCTGGGCACACTGGTTTTCTGGTCGACCGCCCTGCCACAAAGCCAT TTTCAGCACAGTTCTCAGCATTCAAGTGGGGAGGAGGAAGCGACTGACTTGGTCCTGACAGCGAGCTTT GAGCCTGTGAACACAGAGTGGGTTCCCTTTAGAGATGAGAAGTACCCACGACTTGAGGTTCTCCAGGGC TTTGAGCCAGAGCCTGAGATTCTGTCGCCTTTGCAGCCCCCACTCTGTGGGCAAACAGTCTGTGACAAT GTGGAATTGATCTCCCAGTACAATGGATACTGGCCCCTTCGGGGAGAGAAGGTGATACGCTACCAGGTG GTGAACATCTGTGATGATGAGGGCCTAAACCCCATTGTGAGTGAGGAGCAGATTCGTCTGCAGCACGAG GCACTGAATGAGGCCTTCAGCCGCTACAACATCAGCTGGCAGCTGAGCGTCCACCAGGTCCACAATTCC ACCCTGCGACACCGGGTTGTGCTTGTGAACTGTGAGCCCAGCAAGATTGGCAATGACCATTGTGACCCC GAGTGTGAGCACCCACTCACAGGCTATGATGGGGGTGACTGCCGCCTGCAGGGCCGCTGCTACTCCTGG AACCGCAGGGATGGGCTCTGTCACGTGGAGTGTAACAACATGCTGAACGACTTTGACGACGGAGACTGC TGCGACCCCCAGGTGGCTGATGTGCGCAAGACCTGCTTTGACCCTGACTCACCCAAGAGGGCATACATG AGTGTGAAGGAGCTGAAGGAGGCCCTGCAGCTGAACAGTACTCACTTCCTCAACATCTACTTTGCCAGC TCAGTGCGGGAAGACCTTGCAGGTGCTGCCACCTGGCCTTGGGACAAGGACGCTGTCACTCACCTGGGT GGCATTGTCCTCAGCCCAGCATATTATGGGATGCCTGGCCACACCGACACCATGATCCATGAAGTGGGA CATGTTCTGGGACTCTACCATGTCTTTAAAGGAGTCAGTGAAAGAGAATCCTGCAATGACCCCTGCAAG GAGACAGTGCCATCCATGGAAACGGGAGACCTCTGTGCCGACACCGCCCCCACTCCCAAGAGTGAGCTG TGCCGGGAACCAGAGCCCACTAGTGACACCTGTGGCTTCACTCGCTTCCCAGGGGCTCCGTTCACCAAC TACATGAGCTACACGGGTATCACCACTGTCTTGTTTTGTTTTCTGTTAAGAATACATGGGGGCCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_021936 |
Insert Size | 2484 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_021936.2 |
RefSeq Size | 4370 bp |
RefSeq ORF | 2484 bp |
Locus ID | 60676 |
UniProt ID | Q9BXP8 |
Protein Families | Protease, Secreted Protein |
MW | 92.1 kDa |
Gene Summary | This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] Transcript Variant: # This Variant: This variant (2) differs in the 3' UTR and has multiple coding region differences, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222543 | PAPPA2 (Myc-DDK-tagged)-Human pappalysin 2 (PAPPA2), transcript variant 2 |
CNY 6,056.00 |
|
RC222543L3 | Lenti ORF clone of Human pappalysin 2 (PAPPA2), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
RC222543L4 | Lenti ORF clone of Human pappalysin 2 (PAPPA2), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
RG222543 | PAPPA2 (tGFP-tagged) - Human pappalysin 2 (PAPPA2), transcript variant 2 |
CNY 6,370.00 |
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