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PAPP A2 (PAPPA2) (NM_021936) Human Tagged ORF Clone
CAT＃: RG222543
- TrueORF®
PAPPA2 (tGFP-tagged) - Human pappalysin 2 (PAPPA2), transcript variant 2
ORF Plasmid: DDK
"NM_021936" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PAPP-A2; PAPP-E; PAPPE; PLAC3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222543 representing NM_021936
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGCTTAAAGATCCTAAGAATAAGCCTGGCGATTTTGGCTGGGTGGGCACTCTGTTCTGCCAACT CTGAGCTGGGCTGGACACGCAAGAAATCCTTGGTTGAGAGGGAACACCTGAATCAGGTGCTGTTGGAAGG AGAACGTTGTTGGCTGGGGGCCAAGGTTCGAAGACCCAGAGCTTCTCCACAGCATCACCTCTTTGGAGTC TACCCCAGCAGGGCTGGGAACTACCTAAGGCCCTACCCCGTGGGGGAGCAAGAAATCCATCATACAGGAC GCAGCAAACCAGACACTGAAGGAAATGCTGTGAGCCTTGTTCCCCCAGACCTGACTGAAAATCCAGCAGG ACTGAGGGGTGCAGTTGAAGAGCCGGCTGCCCCATGGGTAGGGGATAGTCCTATTGGGCAATCTGAGCTG CTGGGAGATGATGACGCTTATCTCGGCAATCAAAGATCCAAGGAGTCTCTAGGTGAGGCCGGGATTCAGA AAGGCTCAGCCATGGCTGCCACTACTACCACCGCCATTTTCACAACCCTGAACGAACCCAAACCAGAGAC CCAAAGGAGGGGCTGGGCCAAGTCCAGGCAGCGTCGCCAAGTGTGGAAGAGGCGGGCGGAAGATGGGCAG GGAGACTCCGGTATCTCTTCACATTTCCAACCTTGGCCCAAGCATTCCCTTAAACACAGGGTCAAAAAGA GTCCACCGGAGGAAAGCAACCAAAATGGTGGAGAGGGCTCCTACCGAGAAGCAGAGACCTTTAACTCCCA AGTAGGACTGCCCATCTTATACTTCTCTGGGAGGCGGGAGCGGCTGCTGCTGCGTCCAGAAGTGCTGGCT GAGATTCCCCGGGAGGCGTTCACAGTGGAAGCCTGGGTTAAACCGGAGGGAGGACAGAACAACCCAGCCA TCATCGCAGGTGTGTTTGATAACTGCTCCCACACTGTCAGTGACAAAGGCTGGGCCCTGGGGATCCGCTC AGGGAAGGACAAGGGAAAGCGGGATGCTCGCTTCTTCTTCTCCCTCTGCACCGACCGCGTGAAGAAAGCC ACCATCTTGATTAGCCACAGTCGCTACCAACCAGGCACATGGACCCATGTGGCAGCCACTTACGATGGAC GGCACATGGCCCTGTATGTGGATGGCACTCAGGTGGCTAGCAGTCTAGACCAGTCTGGTCCCCTGAACAG CCCCTTCATGGCATCTTGCCGCTCTTTGCTCCTGGGGGGAGACAGCTCTGAGGATGGGCACTATTTCCGT GGACACCTGGGCACACTGGTTTTCTGGTCGACCGCCCTGCCACAAAGCCATTTTCAGCACAGTTCTCAGC ATTCAAGTGGGGAGGAGGAAGCGACTGACTTGGTCCTGACAGCGAGCTTTGAGCCTGTGAACACAGAGTG GGTTCCCTTTAGAGATGAGAAGTACCCACGACTTGAGGTTCTCCAGGGCTTTGAGCCAGAGCCTGAGATT CTGTCGCCTTTGCAGCCCCCACTCTGTGGGCAAACAGTCTGTGACAATGTGGAATTGATCTCCCAGTACA ATGGATACTGGCCCCTTCGGGGAGAGAAGGTGATACGCTACCAGGTGGTGAACATCTGTGATGATGAGGG CCTAAACCCCATTGTGAGTGAGGAGCAGATTCGTCTGCAGCACGAGGCACTGAATGAGGCCTTCAGCCGC TACAACATCAGCTGGCAGCTGAGCGTCCACCAGGTCCACAATTCCACCCTGCGACACCGGGTTGTGCTTG TGAACTGTGAGCCCAGCAAGATTGGCAATGACCATTGTGACCCCGAGTGTGAGCACCCACTCACAGGCTA TGATGGGGGTGACTGCCGCCTGCAGGGCCGCTGCTACTCCTGGAACCGCAGGGATGGGCTCTGTCACGTG GAGTGTAACAACATGCTGAACGACTTTGACGACGGAGACTGCTGCGACCCCCAGGTGGCTGATGTGCGCA AGACCTGCTTTGACCCTGACTCACCCAAGAGGGCATACATGAGTGTGAAGGAGCTGAAGGAGGCCCTGCA GCTGAACAGTACTCACTTCCTCAACATCTACTTTGCCAGCTCAGTGCGGGAAGACCTTGCAGGTGCTGCC ACCTGGCCTTGGGACAAGGACGCTGTCACTCACCTGGGTGGCATTGTCCTCAGCCCAGCATATTATGGGA TGCCTGGCCACACCGACACCATGATCCATGAAGTGGGACATGTTCTGGGACTCTACCATGTCTTTAAAGG AGTCAGTGAAAGAGAATCCTGCAATGACCCCTGCAAGGAGACAGTGCCATCCATGGAAACGGGAGACCTC TGTGCCGACACCGCCCCCACTCCCAAGAGTGAGCTGTGCCGGGAACCAGAGCCCACTAGTGACACCTGTG GCTTCACTCGCTTCCCAGGGGCTCCGTTCACCAACTACATGAGCTACACGGGTATCACCACTGTCTTGTT TTGTTTTCTGTTAAGAATACATGGGGGCCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222543 representing NM_021936
Red=Cloning site Green=Tags(s) MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGV YPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSEL LGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQ GDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLA EIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKA TILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFR GHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEI LSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSR YNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHV ECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAA TWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDL CADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTGITTVLFCFLLRIHGGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021936 |
| ORF Size | 2481 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021936.3 |
| RefSeq Size | 4370 bp |
| RefSeq ORF | 2484 bp |
| Locus ID | 60676 |
| UniProt ID | Q9BXP8 |
| Protein Families | Protease, Secreted Protein |
| Gene Summary | This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222543 | PAPPA2 (Myc-DDK-tagged)-Human pappalysin 2 (PAPPA2), transcript variant 2 |
CNY 6,056.00 |
|
| RC222543L3 | Lenti ORF clone of Human pappalysin 2 (PAPPA2), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC222543L4 | Lenti ORF clone of Human pappalysin 2 (PAPPA2), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| SC318308 | PAPPA2 (untagged)-Human pappalysin 2 (PAPPA2), transcript variant 2 |
CNY 15,870.00 |
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