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SUPT5H (NM_001111020) Human Untagged Clone
CAT＃: SC317210
SUPT5H (untagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SPT5; SPT5H; Tat-CT1 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001111020, the custom clone sequence may differ by one or more nucleotides
ATGTCGGACAGCGAGGACAGCAACTTTTCCGAGGAGGAGGACAGCGAGCGCAGCAGTGAC GGCGAGGAGGCCGAGGTAGACGAAGAGCGGCGGAGTGCAGCGGGCAGTGAGAAAGAAGAA GAGCCTGAGGACGAAGAGGAGGAGGAAGAGGAGGAGGAATACGATGAGGAAGAGGAGGAA GAAGATGATGACCGACCCCCCAAGAAACCCCGCCATGGAGGCTTCATTCTGGACGAGGCT GATGTTGACGATGAGTATGAGGACGAGGACCAGTGGGAGGATGGAGCAGAGGACATTCTA GAGAAAGAAGAGATTGAAGCCTCCAATATCGATAATGTTGTCCTGGATGAAGATCGTTCT GGGGCTCGCCGCCTGCAAAACCTCTGGAGGGACCAGCGAGAAGAAGAACTGGGCGAGTAT TACATGAAGAAATACGCCAAGTCATCTGTGGGAGAGACGGTGTATGGAGGATCTGATGAG CTCTCAGACGACATCACCCAGCAGCAGCTGCTCCCAGGAGTCAAGGATCCCAATCTGTGG ACTGTCAAATGTAAGATTGGGGAGGAACGGGCCACGGCCATTTCCTTGATGCGCAAGTTC ATTGCCTACCAGTTCACAGACACGCCCCTGCAGATCAAGTCAGTAGTGGCACCAGAGCAT GTGAAGGGCTACATCTACGTGGAGGCCTACAAGCAGACCCACGTGAAGCAGGCCATTGAG GGGGTGGGCAACCTGCGGCTTGGCTACTGGAACCAGCAGATGGTGCCCATCAAGGAGATG ACAGACGTGCTCAAAGTGGTGAAGGAGGTGGCCAACCTGAAACCAAAGTCCTGGGTCCGC CTCAAGCGGGGCATCTACAAGGATGACATTGCTCAGGTGGACTACGTGGAGCCCAGCCAG AACACCATCTCCCTGAAGATGATCCCACGCATCGACTACGATCGCATCAAGGCCCGCATG AGCTTGAAAGACTGGTTTGCCAAAAGGAAGAAGTTTAAGCGGCCTCCACAGAGGCTGTTT GATGCTGAGAAGATCAGGTCCCTGGGGGGTGATGTTGCCTCTGATGGTGACTTCCTCATC TTTGAGGGGAACCGTTACAGCCGGAAGGGCTTTCTGTTCAAGAGCTTCGCCATGTCTGCT GTGATCACGGAGGGTGTGAAGCCAACACTCTCTGAGCTGGAAAAGTTTGAGGACCAGCCA GAGGGCATTGACCTGGAGGTGGTGACTGAGAGCACAGGGAAGGAGCGGGAGCACAACTTC CAACCTGGGGACAACGTGGAGGTCTGTGAGGGTGAGCTCATCAACCTGCAGGGCAAGATC CTCAGCGTGGATGGCAACAAGATCACCATCATGCCCAAGCATGAGGACCTCAAGGACATG TTGGAGTTCCCAGCCCAGGAACTTAGAAAATACTTCAAGATGGGGGACCACGTGAAGGTG ATTGCTGGCCGATTCGAGGGCGACACAGGCCTCATTGTGCGGGTGGAGGAGAATTTCGTT ATCCTGTTCTCTGACCTCACCATGCATGAGCTGAAGGTGCTCCCCCGGGACCTGCAGCTC TGCTCAGAGACAGCATCAGGTGTGGATGTTGGGGGCCAGCATGAATGGGGCGAGCTGGTG CAGCTGGATCCCCAGACTGTGGGTGTCATCGTGCGACTAGAACGGGAGACCTTCCAGGTG CTGAACATGTACGGGAAGGTGGTGACTGTCAGACATCAGGCTGTGACCCGGAAGAAGGAC AACCGCTTTGCTGTGGCCTTGGACTCAGAGCAGAACAACATCCATGTGAAAGACATCGTT AAGGTCATTGATGGCCCCCACTCAGGCCGAGAAGGGGAGATTCGCCATCTCTTCCGAAGC TTCGCCTTCCTACATTGCAAGAAACTGGTGGAGAACGGGGGCATGTTTGTCTGCAAGACC CGCCACCTGGTGCTGGCTGGGGGCTCAAAGCCCCGTGATGTGACCAACTTCACCGTGGGT GGCTTTGCGCCTATGAGTCCCCGGATCAGCAGCCCCATGCACCCCAGTGCTGGAGGTCAG CGTGGCGGCTTTGGTAGCCCAGGTGGCGGCAGTGGTGGCATGAGCAGGGGCCGGGGCCGG AGGGACAACGAACTCATCGGCCAGACCGTGCGCATCTCCCAGGGGCCCTACAAAGGCTAC ATCGGTGTGGTGAAAGATGCCACAGAGTCCACGGCCCGTGTGGAGCTGCACTCCACCTGC CAGACCATCTCTGTGGACCGTCAGCGGCTCACCACGGTGGGCTCACGGCGCCCGGGCGGC ATGACCTCGACCTATGGGAGGACGCCCATGTATGGCTCCCAGACGCCCATGTATGGCTCT GGCTCCCGAACACCCATGTACGGCTCACAGACACCCCTCCAGGATGGTAGCCGCACCCCA CACTACGGCTCACAGACGCCCCTGCATGATGGCAGCCGCACTCCTGCCCAGAGTGGGGCC TGGGACCCCAACAACCCCAACACGCCGTCACGGGCTGAGGAAGAATATGAGTATGCTTTC GATGATGAGCCCACCCCGTCCCCGCAGGCCTATGGGGGAACCCCCAATCCCCAAACACCT GGCTACCCAGACCCCTCGTCCCCACAGGTCAACCCACAATACAACCCGCAGACGCCAGGG ACGCCGGCCATGTACAACACAGACCAGTTCTCTCCCTATGCTGCCCCCTCCCCACAAGGT TCCTACCAGCCCAGCCCCAGCCCCCAGAGCTACCACCAGGTGGCGCCAAGCCCAGCAGGC TACCAGAATACCCACTCCCCAGCCAGCTACCACCCTACACCGTCGCCCATGGCCTATCAG GCTAGCCCCAGCCCGAGCCCCGTTGGCTACAGTCCTATGACACCTGGAGCTCCCTCCCCT GGTGGCTACAACCCACACACGCCAGGCTCAGGCATCGAGCAGAACTCCAGCGACTGGGTA ACCACTGACATTCAGGTGAAGGTGCGGGACACCTACCTGGATACACAGGTGGTGGGACAG ACAGGTGTCATCCGCAGTGTCACGGGGGGCATGTGCTCTGTGTACCTGAAGGACAGTGAG AAGGTTGTCAGCATTTCCAGTGAGCACCTGGAGCCTATCACCCCCACCAAGAACAACAAG GTGAAAGTGATCCTGGGCGAGGATCGGGAAGCCACGGGCGTCCTACTGAGCATTGATGGT GAGGATGGCATTGTCCGTATGGACCTTGATGAGCAGCTCAAGATCCTCAACCTCCGCTTC CTGGGGAAGCTCCTGGAAGCC |
| Restriction Sites | Please inquire |
| ACCN | NM_001111020 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001111020.1, NP_001104490.1 |
| RefSeq Size | 3704 bp |
| RefSeq ORF | 3264 bp |
| Locus ID | 6829 |
| UniProt ID | O00267 |
| Protein Families | Transcription Factors |
| Gene Summary | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 5 all encode the same isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226321 | SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2 |
CNY 8,328.00 |
|
| RC226321L3 | Lenti-ORF clone of SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2 |
CNY 7,600.00 |
|
| RC226321L4 | Lenti-ORF clone of SUPT5H (mGFP-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2 |
CNY 7,600.00 |
|
| RG226321 | SUPT5H (tGFP-tagged) - Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 2 |
CNY 6,270.00 |
