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WEE2 (NM_001105558) Human Untagged Clone
CAT＃: SC317119
WEE2 (untagged)-Human WEE1 homolog 2 (S. pombe) (WEE2)
CN¥ 9,120.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | OOMD5; WEE1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317119 representing NM_001105558.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATGACAAAGATATTGACAAAGAACTAAGGCAGAAATTAAACTTTTCCTATTGTGAGGAGACTGAG ATTGAAGGGCAGAAGAAAGTAGAAGAAAGCAGGGAGGCTTCGAGCCAAACCCCAGAGAAGGGTGAAGTG CAGGATTCAGAGGCAAAGGGTACACCACCTTGGACTCCCCTTAGCAACGTGCATGAGCTCGACACATCT TCGGAAAAAGACAAAGAAAGTCCAGATCAGATTTTGAGGACTCCAGTGTCACACCCTCTCAAATGTCCT GAGACACCAGCCCAACCAGACAGCAGGAGCAAGCTGCTGCCCAGTGACAGCCCCTCTACTCCCAAAACC ATGCTGAGCCGGTTGGTGATTTCTCCAACAGGGAAGCTTCCTTCCAGAGGCCCTAAGCATTTGAAGCTC ACACCTGCTCCCCTCAAGGATGAGATGACCTCATTGGCTCTGGTCAATATTAATCCCTTCACTCCAGAG TCCTATAAAAAATTATTTCTTCAATCTGGTGGCAAGAGGAAAATAAGAGGAGATCTTGAGGAAGCTGGT CCAGAGGAAGGCAAGGGAGGGCTGCCTGCCAAGAGATGTGTTTTACGAGAAACCAACATGGCTTCCCGC TATGAAAAAGAATTCTTGGAGGTTGAAAAAATTGGGGTTGGCGAATTTGGTACAGTCTACAAGTGCATT AAGAGGCTGGATGGATGTGTTTATGCAATAAAGCGCTCTATGAAAACTTTTACAGAATTATCAAATGAG AATTCGGCTTTGCATGAAGTTTATGCTCACGCAGTGCTTGGGCATCACCCCCATGTGGTACGTTACTAT TCCTCATGGGCAGAAGATGACCACATGATCATTCAGAATGAATACTGCAATGGTGGGAGTTTGCAAGCT GCTATATCTGAAAACACTAAGTCTGGCAATCATTTTGAAGAGCCAAAACTCAAGGACATCCTTCTACAG ATTTCCCTTGGCCTTAATTACATCCACAACTCTAGCATGGTACACCTGGACATCAAACCTAGTAATATA TTCATTTGTCACAAGATGCAAAGTGAATCCTCTGGAGTCATAGAAGAAGTTGAAAATGAAGCTGATTGG TTTCTCTCTGCCAATGTGATGTATAAAATTGGTGACCTGGGCCACGCAACATCAATAAACAAACCCAAA GTGGAAGAAGGAGATAGTCGCTTCCTGGCTAATGAGATTTTGCAAGAGGATTACCGGCACCTTCCCAAA GCAGACATATTTGCCTTGGGATTAACAATTGCAGTGGCTGCAGGAGCAGAGTCATTGCCCACCAATGGT GCTGCATGGCACCATATCCGCAAGGGTAACTTTCCGGACGTTCCTCAGGAGCTCTCAGAAAGCTTTTCC AGTCTGCTCAAGAACATGATCCAACCTGATGCCGAACAGAGACCTTCTGCAGCAGCTCTGGCCAGAAAT ACAGTTCTCCGGCCTTCCCTGGGAAAAACAGAAGAGCTCCAACAGCAGCTGAATTTGGAAAAGTTCAAG ACTGCCACACTGGAAAGGGAACTGAGAGAAGCCCAGCAGGCCCAGTCACCCCAGGGATATACCCATCAT GGTGACACTGGGGTCTCTGGGACCCACACAGGATCAAGAAGCACAAAACGCCTGGTGGGAGGAAAGAGT GCAAGGTCTTCAAGCTTTACCTCAGGAGAGCGTGAGCCTCTGCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001105558 |
| Insert Size | 1704 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001105558.1 |
| RefSeq Size | 3061 bp |
| RefSeq ORF | 1704 bp |
| Locus ID | 494551 |
| UniProt ID | P0C1S8 |
| Protein Pathways | Cell cycle |
| MW | 62.9 kDa |
| Gene Summary | Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II (PubMed:29606300). Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225964 | WEE2 (Myc-DDK-tagged)-Human WEE1 homolog 2 (S. pombe) (WEE2) |
CN¥ 4,224.00 |
|
| RC225964L1 | Lenti ORF clone of Human WEE1 homolog 2 (S. pombe) (WEE2), Myc-DDK-tagged |
CN¥ 6,624.00 |
|
| RC225964L2 | Lenti ORF clone of Human WEE1 homolog 2 (S. pombe) (WEE2), mGFP tagged |
CN¥ 6,940.00 |
|
| RC225964L3 | Lenti ORF clone of Human WEE1 homolog 2 (S. pombe) (WEE2), Myc-DDK-tagged |
CN¥ 6,940.00 |
|
| RC225964L4 | Lenti ORF clone of Human WEE1 homolog 2 (S. pombe) (WEE2), mGFP tagged |
CN¥ 6,940.00 |
|
| RG225964 | WEE2 (tGFP-tagged) - Human WEE1 homolog 2 (S. pombe) (WEE2) |
CN¥ 5,510.00 |
