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WEE2 (NM_001105558) Human Tagged ORF Clone
CAT＃: RC225964
WEE2 (Myc-DDK-tagged)-Human WEE1 homolog 2 (S. pombe) (WEE2)
ORF Plasmid: tGFP
"NM_001105558" in other vectors (6)
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CNY 4,224.00
CNY 5,040.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OOMD5; WEE1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC225964 representing NM_001105558
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACAAAGATATTGACAAAGAACTAAGGCAGAAATTAAACTTTTCCTATTGTGAGGAGACTGAGA TTGAAGGGCAGAAGAAAGTAGAAGAAAGCAGGGAGGCTTCGAGCCAAACCCCAGAGAAGGGTGAAGTGCA GGATTCAGAGGCAAAGGGTACACCACCTTGGACTCCCCTTAGCAACGTGCATGAGCTCGACACATCTTCG GAAAAAGACAAAGAAAGTCCAGATCAGATTTTGAGGACTCCAGTGTCACACCCTCTCAAATGTCCTGAGA CACCAGCCCAACCAGACAGCAGGAGCAAGCTGCTGCCCAGTGACAGCCCCTCTACTCCCAAAACCATGCT GAGCCGGTTGGTGATTTCTCCAACAGGGAAGCTTCCTTCCAGAGGCCCTAAGCATTTGAAGCTCACACCT GCTCCCCTCAAGGATGAGATGACCTCATTGGCTCTGGTCAATATTAATCCCTTCACTCCAGAGTCCTATA AAAAATTATTTCTTCAATCTGGTGGCAAGAGGAAAATAAGAGGAGATCTTGAGGAAGCTGGTCCAGAGGA AGGCAAGGGAGGGCTGCCTGCCAAGAGATGTGTTTTACGAGAAACCAACATGGCTTCCCGCTATGAAAAA GAATTCTTGGAGGTTGAAAAAATTGGGGTTGGCGAATTTGGTACAGTCTACAAGTGCATTAAGAGGCTGG ATGGATGTGTTTATGCAATAAAGCGCTCTATGAAAACTTTTACAGAATTATCAAATGAGAATTCGGCTTT GCATGAAGTTTATGCTCACGCAGTGCTTGGGCATCACCCCCATGTGGTACGTTACTATTCCTCATGGGCA GAAGATGACCACATGATCATTCAGAATGAATACTGCAATGGTGGGAGTTTGCAAGCTGCTATATCTGAAA ACACTAAGTCTGGCAATCATTTTGAAGAGCCAAAACTCAAGGACATCCTTCTACAGATTTCCCTTGGCCT TAATTACATCCACAACTCTAGCATGGTACACCTGGACATCAAACCTAGTAATATATTCATTTGTCACAAG ATGCAAAGTGAATCCTCTGGAGTCATAGAAGAAGTTGAAAATGAAGCTGATTGGTTTCTCTCTGCCAATG TGATGTATAAAATTGGTGACCTGGGCCACGCAACATCAATAAACAAACCCAAAGTGGAAGAAGGAGATAG TCGCTTCCTGGCTAATGAGATTTTGCAAGAGGATTACCGGCACCTTCCCAAAGCAGACATATTTGCCTTG GGATTAACAATTGCAGTGGCTGCAGGAGCAGAGTCATTGCCCACCAATGGTGCTGCATGGCACCATATCC GCAAGGGTAACTTTCCGGACGTTCCTCAGGAGCTCTCAGAAAGCTTTTCCAGTCTGCTCAAGAACATGAT CCAACCTGATGCCGAACAGAGACCTTCTGCAGCAGCTCTGGCCAGAAATACAGTTCTCCGGCCTTCCCTG GGAAAAACAGAAGAGCTCCAACAGCAGCTGAATTTGGAAAAGTTCAAGACTGCCACACTGGAAAGGGAAC TGAGAGAAGCCCAGCAGGCCCAGTCACCCCAGGGATATACCCATCATGGTGACACTGGGGTCTCTGGGAC CCACACAGGATCAAGAAGCACAAAACGCCTGGTGGGAGGAAAGAGTGCAAGGTCTTCAAGCTTTACCTCA GGAGAGCGTGAGCCTCTGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225964 representing NM_001105558
Red=Cloning site Green=Tags(s) MDDKDIDKELRQKLNFSYCEETEIEGQKKVEESREASSQTPEKGEVQDSEAKGTPPWTPLSNVHELDTSS EKDKESPDQILRTPVSHPLKCPETPAQPDSRSKLLPSDSPSTPKTMLSRLVISPTGKLPSRGPKHLKLTP APLKDEMTSLALVNINPFTPESYKKLFLQSGGKRKIRGDLEEAGPEEGKGGLPAKRCVLRETNMASRYEK EFLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSMKTFTELSNENSALHEVYAHAVLGHHPHVVRYYSSWA EDDHMIIQNEYCNGGSLQAAISENTKSGNHFEEPKLKDILLQISLGLNYIHNSSMVHLDIKPSNIFICHK MQSESSGVIEEVENEADWFLSANVMYKIGDLGHATSINKPKVEEGDSRFLANEILQEDYRHLPKADIFAL GLTIAVAAGAESLPTNGAAWHHIRKGNFPDVPQELSESFSSLLKNMIQPDAEQRPSAAALARNTVLRPSL GKTEELQQQLNLEKFKTATLERELREAQQAQSPQGYTHHGDTGVSGTHTGSRSTKRLVGGKSARSSSFTS GEREPLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001105558 |
| ORF Size | 1701 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001105558.1, NP_001099028.1 |
| RefSeq ORF | 1704 bp |
| Locus ID | 494551 |
| UniProt ID | P0C1S8 |
| Protein Pathways | Cell cycle |
| MW | 62.7 kDa |
| Gene Summary | Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II (PubMed:29606300). Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Homozygous Mutations in WEE2 Cause Fertilization Failure and Female Infertility
,Sang, Q;Li, B;Kuang, Y;,
Am J Hum Genet.
,PubMed ID 29606300
[WEE2]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225964L1 | Lenti ORF clone of Human WEE1 homolog 2 (S. pombe) (WEE2), Myc-DDK-tagged |
CNY 6,624.00 |
|
| RC225964L2 | Lenti ORF clone of Human WEE1 homolog 2 (S. pombe) (WEE2), mGFP tagged |
CNY 6,940.00 |
|
| RC225964L3 | Lenti ORF clone of Human WEE1 homolog 2 (S. pombe) (WEE2), Myc-DDK-tagged |
CNY 6,940.00 |
|
| RC225964L4 | Lenti ORF clone of Human WEE1 homolog 2 (S. pombe) (WEE2), mGFP tagged |
CNY 6,940.00 |
|
| RG225964 | WEE2 (tGFP-tagged) - Human WEE1 homolog 2 (S. pombe) (WEE2) |
CNY 5,510.00 |
|
| SC317119 | WEE2 (untagged)-Human WEE1 homolog 2 (S. pombe) (WEE2) |
CNY 9,120.00 |
