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RNF190 (MARCH10) (NM_001100875) Human Untagged Clone
CAT＃: SC316836
41343 (untagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MARCH-X; MARCH10; RNF190 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316836 representing NM_001100875.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGCATGACGCAAGGGACAGGCAGAAGTTCTTCAGCGATGTTCAGTATCTGCGGGACATGCAGCAT AAGGTGGACTCTGAGTATCAGGCTTGTCTGAGACGACAGGAATATAGAAGAGACCCAAATGAGAAGAAA CGCGATCAGTTTTGGGGGCAAGAGACAAGTTTTGAGAGATCCCGGTTTTCTAGCAGGTCATCTTCCAAA CAGAGTTCTAGTGAGGAGGATGCTCTAACTGAACCAAGGTCATCTATCAAGATATCTGCATTTAAGTGT GACTCCAAACTTCCAGCAATTGACCAAACATCAGTCAAGCAGAAACATAAAAGTACCATGACTGTAAGG AAAGCAGAGAAAGTGGACCCCAGCGAACCCTCTCCAGCAGACCAAGCACCAATGGTTTTATTAAGAAAG AGGAAGCCAAACCTGAGGAGATTTACAGTCAGCCCAGAATCGCACAGCCCGAGAGCATCTGGGGACAGA AGCAGACAGAAACAGCAGTGGCCTGCAAAGGTGCCGGTTCCCAGGGGAGCAGATCAAGTAGTTCAACAA GAAGGCCTGATGTGTAACACTAAGCTGAAGAGGCCAAATCAAGAGAGAAGAAACTTGGTCCCATCGTCA CAGCCAATGACTGAGAACGCCCCTGATAGAGCCAAAAAAGGAGACCCGAGTGCTCCTTCCCAGAGTGAG CTGCATCCAGCCCTGTCCCAGGCCTTCCAAGGAAAAAATAGTCCTCAAGTATTGAGTGAGTTCTCGGGG CCACCACTCACACCCACCACTGTAGGAGGGCCAAGAAAGGCATCATTTAGGTTCCGAGATGAAGACTTT TATTCCATTTTGTCATTGAACAGCAGAAGAGAAAGCGATGACACTGAAGAGGAAACCCAGTCTGAAGAA TGTCTGTGGGTTGGTGTGCGCTCACCCTGTTCTCCTTCCCATCACAAAAGAAGTAGATTTGGGGGGACA TCGACCCCTCAGGCCAAAAATAAAAATTTTGAAGAAAATGCTGAAAATTGCAGAGGTCATTCTTCAAGA AGAAGTGAGCCCAGTCATGGCTCATTGAGAATAAGCAATGCAATGGAGCCAGCAACAGAGCGGCCTTCT GCTGGGCAAAGGTTGTCCCAAGACCCCGGGCTGCCTGATAGGGAATCTGCTACAGAGAAGGACAGAGGT GGCAGTGAAAATGCGAAAAAGAGCCCTCTTTCGTGGGACACCAAATCCGAGCCCAGACAAGAGGTTGGT GTCAATGCTGAAAATGTATGGAGTGATTGTATTTCTGTGGAACATAGGCCTGGCACCCATGATTCTGAA GGATACTGGAAAGACTATTTAAACAGTTCTCAAAATTCTCTTGACTACTTTATTTCTGGCAGACCAATA TCTCCAAGATCATCAGTGAATTCATCCTATAACCCTCCTGCATCATTCATGCATTCTGCTCTGAGAGAT GATATTCCAGTAGACTTGTCAATGTCATCGACTTCAGTTCACAGCTCAGACTCAGAGGGAAACTCAGGG TTTCATGTTTGCCAACCACTGTCTCCCATTAGAAATAGGACTCCATTTGCCAGTGCCGAAAACCATAAT TATTTCCCAGTAAACAGTGCACACGAATTTGCTGTCAGGGAAGCAGAAGATACTACTTTAACAAGCCAG CCTCAGGGGGCTCCACTATATACAGATCTCTTACTAAATCCACAGGGCAATTTGTCCTTAGTGGATTCT TCCAGCTCCTCTCCATCAAGAATGAACTCAGAAGGCCATTTGCATGTGTCTGGGTCTCTGCAAGAAAAT ACACCATTTACTTTCTTTGCAGTGTCTCATTTCCCAAATCAAAATGATAATGGGAGCAGGATGGCAGCC TCTGGTTTCACAGATGAAAAGGAAACCAGTAAGATAAAGGCAGACCCTGAGAAACTCAAGAAATTGCAA GAAAGTCTCCTGGAGGAGGACTCCGAGGAGGAGGGAGACTTGTGTCGCATCTGTCAGATAGCCGGGGGT TCCCCAAGCAACCCCCTCCTGGAGCCTTGCGGCTGTGTGGGAAGCCTGCAGTTTGTTCATCAAGAGTGC CTGAAAAAGTGGCTGAAAGTGAAAATAACATCAGGAGCAGATCTTGGTGCCGTGAAGACCTGTGAGATG TGTAAGCAAGGCCTGCTGGTTGACCTGGGTGACTTTAACATGATTGAGTTCTACCAGAAGCACCAGCAA TCTCAGGCACAAAACGAGCTGATGAATTCAGGCTTGTACCTGGTGCTGCTGCTTCACCTCTATGAGCAG AGGTTTGCAGAACTCATGAGGCTCAACCACAACCAGGTGGAAAGAGAGAGGTTGTCAAGAAATTACCCA CAACCCAGAACAGAGGAAAATGAAAATTCGGAGTTGGGAGATGGAAATGAAGGCAGCATTTCTCAAAGC CAGGTCGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001100875 |
| Insert Size | 2427 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001100875.2 |
| RefSeq Size | 3072 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 162333 |
| UniProt ID | Q8NA82 |
| MW | 90.5 kDa |
| Gene Summary | MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 3. Variants 1 and 2 encode the same isoform (1), which is shorter than isoform 2. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218712 | MARCH10 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2 |
CNY 4,896.00 |
|
| RC218712L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218712L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG218712 | 41343 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2 |
CNY 4,370.00 |
