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RNF190 (MARCH10) (NM_001100875) Human Tagged ORF Clone
CAT＃: RG218712
- TrueORF®
41343 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2
ORF Plasmid: DDK
"NM_001100875" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MARCH-X; MARCH10; RNF190 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218712 representing NM_001100875
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCATGACGCAAGGGACAGGCAGAAGTTCTTCAGCGATGTTCAGTATCTGCGGGACATGCAGCATA AGGTGGACTCTGAGTATCAGGCTTGTCTGAGACGACAGGAATATAGAAGAGACCCAAATGAGAAGAAACG CGATCAGTTTTGGGGGCAAGAGACAAGTTTTGAGAGATCCCGGTTTTCTAGCAGGTCATCTTCCAAACAG AGTTCTAGTGAGGAGGATGCTCTAACTGAACCAAGGTCATCTATCAAGATATCTGCATTTAAGTGTGACT CCAAACTTCCAGCAATTGACCAAACATCAGTCAAGCAGAAACATAAAAGTACCATGACTGTAAGGAAAGC AGAGAAAGTGGACCCCAGCGAACCCTCTCCAGCAGACCAAGCACCAATGGTTTTATTAAGAAAGAGGAAG CCAAACCTGAGGAGATTTACAGTCAGCCCAGAATCGCACAGCCCGAGAGCATCTGGGGACAGAAGCAGAC AGAAACAGCAGTGGCCTGCAAAGGTGCCGGTTCCCAGGGGAGCAGATCAAGTAGTTCAACAAGAAGGCCT GATGTGTAACACTAAGCTGAAGAGGCCAAATCAAGAGAGAAGAAACTTGGTCCCATCGTCACAGCCAATG ACTGAGAACGCCCCTGATAGAGCCAAAAAAGGAGACCCGAGTGCTCCTTCCCAGAGTGAGCTGCATCCAG CCCTGTCCCAGGCCTTCCAAGGAAAAAATAGTCCTCAAGTATTGAGTGAGTTCTCGGGGCCACCACTCAC ACCCACCACTGTAGGAGGGCCAAGAAAGGCATCATTTAGGTTCCGAGATGAAGACTTTTATTCCATTTTG TCATTGAACAGCAGAAGAGAAAGCGATGACACTGAAGAGGAAACCCAGTCTGAAGAATGTCTGTGGGTTG GTGTGCGCTCACCCTGTTCTCCTTCCCATCACAAAAGAAGTAGATTTGGGGGGACATCGACCCCTCAGGC CAAAAATAAAAATTTTGAAGAAAATGCTGAAAATTGCAGAGGTCATTCTTCAAGAAGAAGTGAGCCCAGT CATGGCTCATTGAGAATAAGCAATGCAATGGAGCCAGCAACAGAGCGGCCTTCTGCTGGGCAAAGGTTGT CCCAAGACCCCGGGCTGCCTGATAGGGAATCTGCTACAGAGAAGGACAGAGGTGGCAGTGAAAATGCGAA AAAGAGCCCTCTTTCGTGGGACACCAAATCCGAGCCCAGACAAGAGGTTGGTGTCAATGCTGAAAATGTA TGGAGTGATTGTATTTCTGTGGAACATAGGCCTGGCACCCATGATTCTGAAGGATACTGGAAAGACTATT TAAACAGTTCTCAAAATTCTCTTGACTACTTTATTTCTGGCAGACCAATATCTCCAAGATCATCAGTGAA TTCATCCTATAACCCTCCTGCATCATTCATGCATTCTGCTCTGAGAGATGATATTCCAGTAGACTTGTCA ATGTCATCGACTTCAGTTCACAGCTCAGACTCAGAGGGAAACTCAGGGTTTCATGTTTGCCAACCACTGT CTCCCATTAGAAATAGGACTCCATTTGCCAGTGCCGAAAACCATAATTATTTCCCAGTAAACAGTGCACA CGAATTTGCTGTCAGGGAAGCAGAAGATACTACTTTAACAAGCCAGCCTCAGGGGGCTCCACTATATACA GATCTCTTACTAAATCCACAGGGCAATTTGTCCTTAGTGGATTCTTCCAGCTCCTCTCCATCAAGAATGA ACTCAGAAGGCCATTTGCATGTGTCTGGGTCTCTGCAAGAAAATACACCATTTACTTTCTTTGCAGTGTC TCATTTCCCAAATCAAAATGATAATGGGAGCAGGATGGCAGCCTCTGGTTTCACAGATGAAAAGGAAACC AGTAAGATAAAGGCAGACCCTGAGAAACTCAAGAAATTGCAAGAAAGTCTCCTGGAGGAGGACTCCGAGG AGGAGGGAGACTTGTGTCGCATCTGTCAGATAGCCGGGGGTTCCCCAAGCAACCCCCTCCTGGAGCCTTG CGGCTGTGTGGGAAGCCTGCAGTTTGTTCATCAAGAGTGCCTGAAAAAGTGGCTGAAAGTGAAAATAACA TCAGGAGCAGATCTTGGTGCCGTGAAGACCTGTGAGATGTGTAAGCAAGGCCTGCTGGTTGACCTGGGTG ACTTTAACATGATTGAGTTCTACCAGAAGCACCAGCAATCTCAGGCACAAAACGAGCTGATGAATTCAGG CTTGTACCTGGTGCTGCTGCTTCACCTCTATGAGCAGAGGTTTGCAGAACTCATGAGGCTCAACCACAAC CAGGTGGAAAGAGAGAGGTTGTCAAGAAATTACCCACAACCCAGAACAGAGGAAAATGAAAATTCGGAGT TGGGAGATGGAAATGAAGGCAGCATTTCTCAAAGCCAGGTCGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218712 representing NM_001100875
Red=Cloning site Green=Tags(s) MLHDARDRQKFFSDVQYLRDMQHKVDSEYQACLRRQEYRRDPNEKKRDQFWGQETSFERSRFSSRSSSKQ SSSEEDALTEPRSSIKISAFKCDSKLPAIDQTSVKQKHKSTMTVRKAEKVDPSEPSPADQAPMVLLRKRK PNLRRFTVSPESHSPRASGDRSRQKQQWPAKVPVPRGADQVVQQEGLMCNTKLKRPNQERRNLVPSSQPM TENAPDRAKKGDPSAPSQSELHPALSQAFQGKNSPQVLSEFSGPPLTPTTVGGPRKASFRFRDEDFYSIL SLNSRRESDDTEEETQSEECLWVGVRSPCSPSHHKRSRFGGTSTPQAKNKNFEENAENCRGHSSRRSEPS HGSLRISNAMEPATERPSAGQRLSQDPGLPDRESATEKDRGGSENAKKSPLSWDTKSEPRQEVGVNAENV WSDCISVEHRPGTHDSEGYWKDYLNSSQNSLDYFISGRPISPRSSVNSSYNPPASFMHSALRDDIPVDLS MSSTSVHSSDSEGNSGFHVCQPLSPIRNRTPFASAENHNYFPVNSAHEFAVREAEDTTLTSQPQGAPLYT DLLLNPQGNLSLVDSSSSSPSRMNSEGHLHVSGSLQENTPFTFFAVSHFPNQNDNGSRMAASGFTDEKET SKIKADPEKLKKLQESLLEEDSEEEGDLCRICQIAGGSPSNPLLEPCGCVGSLQFVHQECLKKWLKVKIT SGADLGAVKTCEMCKQGLLVDLGDFNMIEFYQKHQQSQAQNELMNSGLYLVLLLHLYEQRFAELMRLNHN QVERERLSRNYPQPRTEENENSELGDGNEGSISQSQVV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100875 |
| ORF Size | 2424 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100875.3 |
| RefSeq Size | 3069 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 162333 |
| UniProt ID | Q8NA82 |
| Gene Summary | MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218712 | MARCH10 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2 |
CNY 4,896.00 |
|
| RC218712L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218712L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC316836 | 41343 (untagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 2 |
CNY 7,220.00 |
