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JHDM1D (KDM7A) (NM_030647) Human Untagged Clone
CAT＃: SC310699
JHDM1D (untagged)-Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D)
CNY 6,896.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | JHDM1D |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_030647 edited
ATGGCCGGAGCGGCGGCGGCGGTGGCCGCGGGAGCAGCAGCTGGAGCCGCCGCGGCAGCC GTGTCGGTGGCGGCTCCCGGCCGGGCCTCGGCGCCTCCGCCGCCCCCGCCCGTGTACTGT GTGTGCCGGCAGCCGTACGACGTGAACCGCTTCATGATCGAGTGCGATATCTGCAAGGAC TGGTTCCACGGCAGCTGTGTTGGAGTAGAAGAACATCATGCTGTTGACATTGACCTGTAT CACTGTCCCAACTGTGCAGTTTTACATGGTTCCTCCTTGATGAAAAAAAGGAGGAACTGG CACAGACATGACTACACAGAAATTGATGATGGTTCCAAACCAGTGCAAGCTGGAACTAGA ACTTTCATTAAGGAATTACGCTCTCGAGTCTTCCCAAGTGCCGATGAAATAATTATAAAG ATGCATGGCAGCCAGCTGACACAAAGATATCTGGAGAAACATGGATTTGATGTCCCTATT ATGGTCCCAAAATTAGATGATCTAGGACTCAGGCTCCCTTCACCTACATTTTCTGTGATG GATGTGGAACGTTATGTAGGTGGTGACAAAGTGATAGATGTCATTGATGTGGCGAGGCAG GCAGACAGCAAAATGACACTTCACAATTATGTTAAATACTTCATGAATCCTAACAGACCA AAAGTGTTAAATGTGATCAGCCTTGAATTTTCAGATACAAAGATGTCTGAATTGGTGGAG GTCCCTGATATAGCCAAAAAACTTTCCTGGGTGGAAAATTATTGGCCAGATGATTCAGTC TTTCCCAAGCCATTTGTTCAGAAATATTGCTTAATGGGAGTTCAAGACAGCTATACAGAT TTCCACATTGACTTCGGTGGAACTTCAGTCTGGTACCATGTCCTCTGGGGTGAGAAGATT TTTTATTTAATAAAGCCAACAGATGAAAATTTGGCACGTTATGAATCTTGGAGTTCATCT GTGACCCAGAGTGAGGTGTTCTTTGGAGATAAGGTGGATAAATGCTACAAATGTGTGGTA AAGCAGGGACATACCTTATTTGTTCCTACAGGGTGGATCCATGCTGTGCTCACTTCTCAG GACTGTATGGCTTTTGGGGGGAACTTCCTGCACAACCTTAACATTGGCATGCAGCTCAGG TGTTATGAGATGGAGAAAAGGCTAAAAACACCAGATCTTTTCAAATTCCCTTTCTTTGAA GCCATATGTTGGTTTGTAGCCAAAAACTTGCTGGAAACCCTGAAAGAACTGAGAGAAGAT GGTTTCCAGCCTCAAACTTACCTAGTACAGGGAGTGAAAGCACTGCATACTGCTTTAAAA TTATGGATGAAAAAAGAACTTGTATCTGAACATGCCTTTGAAATTCCAGACAATGTTAGA CCTGGACACCTTATTAAAGAACTTTCTAAAGTAATTCGAGCAATAGAGGAGGAAAACGGC AAACCAGTTAAATCTCAGGGAATTCCTATTGTGTGTCCAGTTTCACGATCCTCAAATGAA GCAACTTCCCCATACCATTCCCGAAGAAAGATGAGGAAACTTCGAGATCATAATGTCCGA ACTCCTTCTAACCTAGACATCCTAGAGCTCCACACAAGGGAGGTCCTCAAAAGATTAGAG ATGTGTCCATGGGAAGAGGACATCTTGAGCTCTAAACTGAATGGAAAATTCAACAAACAT CTCCAACCATCCTCCACAGTACCTGAATGGAGAGCGAAAGATAATGATCTACGATTACTG CTGACAAATGGAAGAATAATTAAAGATGAAAGGCAGCCCTTTGCAGATCAAAGTCTTTAT ACAGCAGATAGTGAAAATGAAGAGGATAAAAGAAGGACAAAAAAGGCAAAAATGAAGATA GAAGAGAGTTCAGGAGTAGAGGGAGTGGAACATGAAGAATCTCAAAAACCACTGAATGGG TTTTTTACACGTGTGAAATCAGAACTCAGGAGTAGATCATCAGGATATTCTGATATTTCT GAGTCAGAAGACTCCGGACCCGAGTGCACTGCACTGAAAAGTATCTTTACCACTGAAGAG TCTGAAAGTTCAGGTGATGAAAAGAAACAAGAAATAACATCCAACTTTAAGGAGGAATCT AATGTGATGAGGAACTTCCTTCAAAAGAGCCAGAAGCCATCTAGAAGTGAAATTCCAATT AAAAGGGAATGTCCTACCTCGACGAGCACAGAGGAAGAAGCTATTCAGGGCATGCTGTCT ATGGCAGGGTTGCACTATTCCACGTGTTTACAAAGGCAAATACAAAGCACAGACTGCAGT GGTGAAAGAAACTCTCTCCAGGATCCCAGCAGCTGCCATGGCAGTAACCATGAGGTTAGG CAGTTGTATCGCTATGATAAACCAGTGGAATGTGGATACCATGTCAAGACTGAAGATCCA GACTTGAGGACTTCCTCCTGGATTAAACAGTTTGATACTTCCAGATTTCATCCTCAGGAT CTAAGTAGAAGCCAGAAATGCATCAGAAAGGAAGGTTCATCAGAAATTAGTCAGAGGGTA CAAAGTAGGAATTATGTGGACAGCAGCGGCTCAAGCCTTCAGAATGGAAAGTATATGCAG AATTCAAACCTGACTTCGGGGGCGTGCCAGATAAGTAATGGCAGTCTAAGCCCAGAAAGG CCAGTTGGTGAAACTTCCTTCTCGGTGCCCCTTCACCCCACCAAGAGACCGGCATCAAAT CCACCACCTATCAGCAACCAGGCAACAAAAGGTAAACGTCCAAAAAAAGGAATGGCAACA GCCAAACAACGTCTTGGGAAGATCCTTAAGTTGAACAGAAATGGCCATGCACGTTTCTTT GTGTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_030647 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030647.1, NP_085150.1 |
| RefSeq Size | 9178 bp |
| RefSeq ORF | 2826 bp |
| Locus ID | 80853 |
| UniProt ID | Q6ZMT4 |
| Gene Summary | Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218944 | JHDM1D (Myc-DDK-tagged)-Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D) |
CNY 6,896.00 |
|
| RC218944L1 | Lenti ORF clone of Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D), Myc-DDK-tagged |
CNY 9,296.00 |
|
| RC218944L2 | Lenti ORF clone of Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D), mGFP tagged |
CNY 9,030.00 |
|
| RC218944L3 | Lenti ORF clone of Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC218944L4 | Lenti ORF clone of Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D), mGFP tagged |
CNY 9,030.00 |
|
| RG218944 | JHDM1D (tGFP-tagged) - Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D) |
CNY 7,890.00 |
