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GRLF1 (ARHGAP35) (NM_004491) Human Untagged Clone
CAT＃: SC308989
ARHGAP35 (untagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1)
CNY 28,880.00
货期*
4周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP; p190RhoGAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308989 representing NM_004491.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGATGGCAAGAAAGCAAGATGTCCGAATTCCCACCTACAACATCAGTGTGGTGGGATTATCTGGG ACCGAGAAGGAAAAGGGCCAGTGTGGGATTGGAAAGTCTTGTTTGTGCAACCGCTTCGTGCGCCCGAGT GCTGACGAGTTTCACTTGGACCATACCTCCGTCCTCAGCACCAGTGACTTTGGAGGGCGAGTGGTCAAT AATGACCACTTTCTCTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGATTGTGTGGAATGTAAGATGCAC ATTGTGGAGCAGACTGAATTTATTGATGATCAGACTTTTCAACCTCATCGAAGCACGGCCCTGCAGCCC TATATCAAGAGAGCTGCTGCGACCAAGCTTGCATCAGCTGAAAAACTCATGTACTTTTGCACTGACCAG CTGGGGCTGGAGCAGGACTTTGAGCAGAAACAAATGCCAGACGGAAAGCTGCTGGTTGATGGTTTTCTT CTTGGTATTGATGTTAGCAGGGGCATGAATAGGAACTTTGATGACCAGCTCAAGTTTGTCTCCAATCTC TACAATCAGCTTGCAAAAACAAAAAAGCCCATAGTGGTGGTCCTGACTAAGTGTGACGAAGGTGTTGAG CGGTACATTAGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTGGAGACCTCAGCG AGATCCAATGTAAACGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAAAGTCGGGGAAAG ACAAAAATCATTCCTTATTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAAGACAAG TATGAGTGGCTGGTGAGTCGCATTGTGAAAAACCACAATGAGAACTGGCTGAGTGTCAGCCGAAAGATG CAGGCCTCTCCAGAATACCAGGACTATGTCTACCTGGAAGGGACTCAGAAAGCCAAGAAGCTGTTTCTA CAGCACATCCACCGCCTCAAGCATGAGCATATCGAGCGTAGGAGAAAGCTGTACCTGGCAGCCCTGCCA TTAGCTTTTGAAGCTCTTATACCTAATCTAGATGAAATAGACCACCTAAGCTGCATAAAAGCCAAAAAG CTCTTAGAAACCAAGCCAGAATTCTTGAAGTGGTTTGTTGTGCTTGAAGAGACCCCATGGGATGCCACC AGTCACATTGACAACATGGAAAACGAACGGATTCCCTTTGATTTAATGGATACCGTCCCTGCAGAGCAG CTATACGAGGCCCACTTAGAGAAGCTGAGGAACGAAAGGAAAAGAGTTGAGATGCGAAGGGCGTTTAAA GAAAACCTGGAGACTTCTCCTTTCATAACTCCCGGAAAGCCTTGGGAAGAGGCCCGTAGTTTTATTATG AATGAGGATTTCTACCAGTGGCTGGAGGAATCTGTATACATGGATATTTATGGCAAACACCAAAAGCAA ATTATAGATAAAGCAAAGGAAGAATTTCAGGAGTTGCTTTTGGAATATTCAGAATTGTTTTATGAACTG GAGCTGGATGCTAAGCCCAGCAAGGAGAAGATGGGTGTTATTCAGGATGTTCTGGGAGAGGAACAGCGA TTTAAAGCATTACAAAAGCTCCAAGCAGAGCGTGATGCCCTTATTCTGAAACACATTCATTTTGTGTAC CACCCAACAAAGGAGACATGCCCCAGCTGCCCAGCTTGTGTGGACGCTAAGATTGAGCACTTGATTAGT TCTCGGTTTATCCGGCCGTCTGACCGGAATCAGAAAAATTCACTCTCTGACCCTAACATTGATAGAATC AACTTGGTTATATTGGGCAAAGACGGCCTTGCCCGAGAGTTGGCCAATGAGATTCGAGCTCTTTGTACA AATGATGACAAGTATGTGATAGATGGTAAAATGTATGAGCTTTCCCTGAGGCCAATAGAGGGGAATGTC AGGCTTCCTGTGAACTCTTTCCAGACGCCAACATTTCAGCCCCACGGCTGTCTCTGCCTTTACAATTCA AAGGAATCGCTATCCTATGTAGTGGAAAGTATAGAGAAGAGTAGAGAGTCCACGCTGGGCCGGCGGGAT AATCATTTAGTCCATCTCCCCCTTACATTAATTTTGGTTAACAAGAGAGGAGACACCAGTGGAGAGACT CTGCATAGCTTAATACAGCAAGGTCAACAAATTGCTAGCAAACTTCAGTGTGTCTTTCTCGACCCTGCT TCTGCTGGCATTGGTTACGGACGCAACATTAATGAAAAGCAAATCAGTCAAGTTTTGAAGGGACTCCTG GACTCTAAGCGTAACTTAAACCTGGTCAGTTCTACTGCTAGCATCAAAGATTTGGCTGATGTTGATCTG CGAATTGTTATGTGTCTGATGTGTGGAGATCCTTTTAGTGCAGATGACATACTTTTTCCTGTCCTTCAG TCCCAAACCTGTAAATCTTCCCATTGTGGAAGCAACAACTCTGTTTTACTTGAACTACCAATCGGACTG CACAAGAAGCGGATTGAACTGTCTGTTCTTTCATACCATTCCTCCTTTAGCATCAGAAAGAGCCGGTTG GTTCATGGGTACATTGTTTTTTATTCAGCCAAACGTAAGGCCTCTTTGGCTATGTTACGTGCCTTTCTT TGTGAAGTGCAGGATATTATCCCTATTCAGCTTGTAGCACTCACTGATGGCGCTGTAGATGTCCTGGAC AATGACTTAAGTAGGGAACAGCTAACTGAGGGGGAGGAGATTGCTCAAGAAATTGACGGAAGGTTCACA AGCATCCCCTGTAGCCAACCCCAGCATAAACTTGAGATCTTTCACCCATTTTTTAAAGATGTGGTGGAA AAAAAGAACATAATCGAGGCTACTCATATGTACGATAATGCTGCCGAGGCCTGTAGCACCACCGAAGAG GTGTTTAACTCCCCCCGGGCAGGATCACCGCTCTGCAACTCAAACCTGCAGGATTCAGAAGAAGATATC GAGCCATCTTACAGCCTGTTTCGAGAAGACACATCACTGCCTTCTCTGTCCAAAGACCATTCTAAGCTC TCTATGGAACTGGAGGGAAATGATGGGCTGTCTTTCATTATGAGCAATTTTGAGAGTAAACTGAACAAC AAAGTACCTCCGCCAGTCAAACCAAAGCCTCCTGTCCATTTTGAAATTACAAAGGGGGATCTATCTTAT TTAGACCAAGGCCATAGGGATGGACAGAGGAAGTCTGTGTCTTCTAGCCCCTGGCTGCCTCAGGATGGG TTTGATCCTTCTGACTATGCTGAACCCATGGATGCTGTGGTGAAGCCAAGGAATGAAGAAGAAAACATA TACTCCGTGCCCCATGACAGCACCCAAGGCAAAATCATCACCATTCGGAATATCAACAAAGCCCAGTCC AACGGCAGCGGGAATGGTTCTGACAGTGAAATGGACACCAGCTCTCTAGAGCGAGGGCGCAAGGTTTCC ATCGTGAGCAAGCCAGTGCTGTACAGGACGAGATGCACCCGGCTGGGGCGGTTTGCTAGTTACCGGACC AGCTTCAGCGTGGGGAGTGATGATGAGCTGGGGCCCATCCGGAAGAAAGAGGAGGATCAGGCATCCCAG GGTTATAAAGGGGACAATGCTGTCATTCCATACGAAACAGACGAAGACCCGCGGAGGAGGAATATTCTT CGCAGCCTAAGGAGGAACACTAAGAAACCAAAGCCCAAACCCCGGCCATCCATCACAAAGGCAACCTGG GAGAGTAACTATTTTGGGGTGCCCTTAACAACTGTCGTGACTCCAGAGAAGCCGATCCCCATTTTTATT GAAAGATGTATTGAGTACATTGAAGCCACAGGACTGAGCACGGAAGGCATCTACCGGGTCAGCGGGAAC AAGTCTGAGATGGAGAGTCTGCAGAGACAGTTTGATCAAGACCACAACCTGGACCTGGCAGAGAAAGAC TTTACGGTGAATACCGTGGCTGGTGCCATGAAGAGCTTTTTCTCAGAACTGCCTGACCCCCTGGTCCCG TATAACATGCAGATCGACTTGGTGGAAGCACACAAAATCAACGACCGGGAGCAGAAGTTGCATGCCCTT AAGGAGGTATTAAAGAAATTTCCAAAGGAAAACCACGAAGTCTTCAAGTATGTCATCTCTCACCTAAAC AAGGTCAGCCACAACAACAAGGTGAATCTCATGACCAGCGAGAACCTCTCCATCTGCTTCTGGCCCACC TTGATGAGACCTGATTTCAGCACTATGGACGCCCTCACAGCCACGCGCACCTACCAGACAATCATTGAA CTCTTTATCCAGCAGTGCCCCTTCTTCTTCTACAATCGGCCCATCACCGAGCCCCCCGGCGCCAGGCCC AGCTCCCCCTCTGCCGTGGCTTCCACCGTCCCCTTCCTCACTTCCACGCCTGTCACAAGTCAGCCGTCG CCCCCACAGTCGCCTCCACCCACCCCCCAGTCCCCAATGCAGCCACTGCTTCCCTCCCAGCTTCAAGCC GAACACACGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_004491 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004491.4 |
| RefSeq Size | 8904 bp |
| RefSeq ORF | 4500 bp |
| Locus ID | 2909 |
| UniProt ID | Q9NRY4 |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton |
| MW | 170.5 kDa |
| Gene Summary | The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate segment in the coding region compared to variant 1, resulting in a longer isoform (b) that has a distinct C-terminus compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222277 | ARHGAP35 (Myc-DDK-tagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1) |
CNY 10,360.00 |
|
| RC222277L3 | Lenti-ORF clone of ARHGAP35 (Myc-DDK-tagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1) |
CNY 12,730.00 |
|
| RC222277L4 | Lenti-ORF clone of ARHGAP35 (mGFP-tagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1) |
CNY 12,730.00 |
|
| RG222277 | ARHGAP35 (tGFP-tagged) - Human glucocorticoid receptor DNA binding factor 1 (GRLF1) |
CNY 11,880.00 |
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