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GRLF1 (ARHGAP35) (NM_004491) Human Tagged ORF Clone
CAT＃: RG222277
- TrueORF®
ARHGAP35 (tGFP-tagged) - Human glucocorticoid receptor DNA binding factor 1 (GRLF1)
ORF Plasmid: DDK
"NM_004491" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP; p190RhoGAP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222277 representing NM_004491
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGGCAAGAAAGCAAGATGTCCGAATTCCCACCTACAACATCAGTGTGGTGGGATTATCTGGGA CCGAGAAGGAAAAGGGCCAGTGTGGGATTGGAAAGTCTTGTTTGTGCAACCGCTTCGTGCGCCCGAGTGC TGACGAGTTTCACTTGGACCATACCTCCGTCCTCAGCACCAGTGACTTTGGAGGGCGAGTGGTCAATAAT GACCACTTTCTCTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGATTGTGTGGAATGTAAGATGCACATTG TGGAGCAGACTGAATTTATTGATGATCAGACTTTTCAACCTCATCGAAGCACGGCCCTGCAGCCCTATAT CAAGAGAGCTGCTGCGACCAAGCTTGCATCAGCTGAAAAACTCATGTACTTTTGCACTGACCAGCTGGGG CTGGAGCAGGACTTTGAGCAGAAACAAATGCCAGACGGAAAGCTGCTGGTTGATGGTTTTCTTCTTGGTA TTGATGTTAGCAGGGGCATGAATAGGAACTTTGATGACCAGCTCAAGTTTGTCTCCAATCTCTACAATCA GCTTGCAAAAACAAAAAAGCCCATAGTGGTGGTCCTGACTAAGTGTGACGAAGGTGTTGAGCGGTACATT AGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTGGAGACCTCAGCGAGATCCAATG TAAACGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAAAGTCCGGGGAAGACAAAAATCAT TCCTTATTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAAGACAAGTATGAGTGGCTG GTGAGTCGCATTGTGAAAAACCACAATGAGAACTGGCTGAGTGTCAGCCGAAAGATGCAGGCCTCTCCAG AATACCAGGACTATGACTACCTGGAAGGGACTCAGAAAGCCAAGAAGCTGTTTTTACAGCACATCCACCG CCTCAAGCATGAGCATATCGAGCGTAGGAGAAAGCTGTACCTGGCAGCCCTGCCATTAGCTTTTGAAGCT CTTATACCTAATCTAGATGAAATAGACCACCTAGGCTGCATAAAAGCCAAAAAGCTCTTAGAAACCAAGC CAGAATTCTTGAAGTGGTTTGTTGTGCTTGAAGAGACCCCATGGGATGCCACCAGTCACATTGACAACAT GGAAAACGAACGGATTCCCTTTGATTTAATGGATACCGTCCCTGCAGAGGCACTATACGAGGCCCACTTA GAGAAGCTGAGGAACGAAAGGAAAAGAGTTGAGATGCGAAGGGCGTTTAAAGAAAACCTGGAGACTTCTC CTTTCATAACTCCCGGAAAGCCTTGGGAAGAGGCCCGTAGTTTTATTATGAATGAGGATTTCTACCAGTG GCTGGAGGAATCTGTATACACGGATATTTATGGCAAACACCAAAAGCAAATTATAGATAAAGCAAAGGAA GAATTTCAGGAGTTGCTTTTGGAATATTCAGAATTGTTTTATGAACTGGAGCTGGATGCTAAGCCCAGCA AGGAGAAGATGGGTGTTATTCAGGATGTTCTGGGAGAGGAACAGCGATTTAAAGCCATTTACAAAAGCTC CAAGCAGAGCGTTGATGCCCTTATTCTGAAACACATTCATTTTGTGTACCACCCAACAAAGGAGACATGC CCCAGCTGCCCAGCTTGTGTGGACGCTAAGATTGAGCACTTGATTAGTTCTCGGTTTATCCGGCCGTCTG ACCGGAATCAGAAAAATTCACTCTCTGACCCTAACATTGATAGAATCAACTTGGTTATATTGGGCAAAGA CGCCTTGCCCGAGAGTTGGCCAATGGAGATTAGAGCTCTTTGTACAAATGATGACAAGTATGTGATAGAT GGTAAAATGTATGAGCTTTCCCTGAGGCCAATAGAGGGGAATGTCAGGCTTCCTGTGAACTCTTTCCAGA CGCCAACATTTCAGCCCCACGGCTGTCTCTGCCTTTACAATTCAAAGGAATCGCTATCCTATGTAGTGGA AAGTATAGAGAAGAGTAGAGAGTCCACGCTGGGCCGGCGGGATAATCATTTAGTCCATCTCCCCCTTACA TTAATTTTGGTTAACAAGAGAGGAGACACCAGTGGAGAGACTCTGCATAGCTTAATACAGCAAGGTCAAC AAATTGCTAGCAAACTTCAGTGTGTCTTTCTCGACCCTGCTTCTGCTGGCATTGGTTACGGACGCAACAT TAATGAAAAGCAAATCAGTCAAGTTTTGAAGGGACTCCTGGACTCTAAGCGTAACTTAAACCTGGTCAGT TCTACTGCTAGCATCAAAGATTTGGCTGATGTTGATCTGCGAATTGTTATGTGTCTGATGTGTGGAGATC CTTTTAGTGCAGATGATATACTTTTTCCTGTCCTTCAGTCCCAAACCTGTAAATCTTCCCATTGTGGAAG CAACAACTCTGTTTTACTTGAACTACCAATCGGACTGCACAAGAAGCGGATTGAACTGTCTGTTCTTTCA TACCATTCCTCCTTTAGCATCAGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCAGCCAAAC GTAAGGCCTCTTTGGCTATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATTATCCCTATTCAGCTTGT AGCACTCACTGATGGCGCTGTAGATGTCCTGGACAATGACTTAAGTAGGGAACAGCTAACTGAGGGGGAG GAGATTGCTCAAGAAATTGACGGAAGGTTCACAAGCATCCCCTGTAGCCAACCCCAGCATAAACTTGAGA TCTTTCACCCATTTTTTAAAGATGTGGTGGAAAAAAAGAACATAATCGAGGCTACTCATATGTACGATAA TGCTGCCGAGGCCTGTAGCACCACCGAAGAGGTGTTTAACTCCCCCCGGGCAGGATCACCGCTCTCGAAC TCAAACCTGCAGGATTCAGAAGAAGATATCGAGCCATCTTACAGCCTGTTTCGAGAAGACACATCACTGC CTTCTCTGTCCAAAGACCATTCTAAGCTCTCTATGGAACTGGAGGGAAATGATGGGCTGTCTTTCATTAT GAGCAATTTTGAGAGTAAACTGAACAACAAAGTACCTCCGCCAGTCAAACCAAAGCCTCCTGTCCATTTT GAAATTACAAAGGGGGATCTATCTTATTTAGACCAAGGCCATAGGGATGGACAGAGGAAGTCTGTGTCTT CTAGCCCCTGGCTGCCTCAGGATGGGTTTGATCCTTCTGACTATGCTGAACCCATGGATGCTGTGGTGAA GCCAAGGAATGAAGAAGAAAACATATACTCCGTGCCCCATGACAGCACCCAAGGCAAAATCATCACCATT CGGAATATCAACAAAGCCCAGTCCAACGGCAGCGGGAATGGTTCTGACAGTGAAATGGACACCAGCTCTC TAGAGCGAGGGCGCAAGGTTTCCATCGTGAGCAAGCCAGTGCTGTACAGGACGAGATGCACCCGGCTGGG GCGGTTTGCTAGTTACCGGACCAGCTTCAGCGTCGGGAGTGATGATGAGCTGGGGCCCATCCGGAAGAAA GAGGAGGATCAGGCATCCCAGGGTTATAAAGGGGACAATGCTGTCATTCCATACGAAACAGACGAAGACC CGCGGAGGAGGAATATTCTTCGCAGCCTAAGGAGGAACACTAAGAAACCAAAGCCCAAACCCCGGCCATC CATCACAAAGGCAACCTGGGAGAGTAACTATTTTGGGGTGCCCTTAACAACTGTCGTGACTCCAGAGAAG CCGATCCCCATTTTTATTGAAAGATGTATTGAGTACATTGAAGCCACAGGACTGAGCACGGAAGGCATCT ACCGGGTCAGCGGGAACAAGTCTGAGATCGAGAGTCTGCAGAGACAGTTTGATCAAGACCACAACCTGGA CCTGGCAGAGAAAGACTTTACGGTGAATACCGTGGCTGGTGCCATGAAGAGCTTTTTCTCAGAACTGCCT GACCCCCTGGTCCCGTATAACATGCAGATCGACTTGGTGGAAGCACACAAAATCAACGACCGGGAGCAGA AGTTGCATGCCCTTAAGGAGGTATTAAAGAAATTTCCAAAGGAAAACCACGAAGTCTTCAAGTATGTCAT CTCTCACCTAAACAAGGTCAGCCACAACAACAAGGTGAATCTCATGACCAGCGAGAACCTCTCCATCTGC TTCTGGCCCACCTTGATGAGACCTGATTTCAGCACTATGGACGCCCTCACAGCCACGCGCACCTACCAGA CAATCATTGAACTCTTTATCCAGCAGTGCCCCTTCTTCTTCTACAATCGGCCCATCACCGAGCCCCCCGG CGCCAGGCCCAGCTCCCCCTCTGCCGTGGCTTCCACCGTCCCCTTCCTCACTTCCACGCCTGTCACAAGT CAGCCGTCGCCCCCACAGTCGCCTCCACCCACCCCCCAGTCCCCAATGCAGCCACTGCTTCCCTCCCCGC TTCATCCCCACCGTCCCACTCCACAGCCTTCCCGAAACATTCCCTGGCAAACAAAGGAACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222277 representing NM_004491
Red=Cloning site Green=Tags(s) MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNN DHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLG LEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYI RDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSPGKTKIIPYFEALKQQSQQIATAKDKYEWL VSRIVKNHNENWLSVSRKMQASPEYQDYDYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEA LIPNLDEIDHLGCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEALYEAHL EKLRNERKRVEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYTDIYGKHQKQIIDKAKE EFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKAIYKSSKQSVDALILKHIHFVYHPTKETC PSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDALPESWPMEIRALCTNDDKYVID GKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLT LILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVS STASIKDLADVDLRIVMCLMCGDPFSADDILFPVLQSQTCKSSHCGSNNSVLLELPIGLHKKRIELSVLS YHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAVDVLDNDLSREQLTEGE EIAQEIDGRFTSIPCSQPQHKLEIFHPFFKDVVEKKNIIEATHMYDNAAEACSTTEEVFNSPRAGSPLSN SNLQDSEEDIEPSYSLFREDTSLPSLSKDHSKLSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHF EITKGDLSYLDQGHRDGQRKSVSSSPWLPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITI RNINKAQSNGSGNGSDSEMDTSSLERGRKVSIVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKK EEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEK PIPIFIERCIEYIEATGLSTEGIYRVSGNKSEIESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELP DPLVPYNMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSIC FWPTLMRPDFSTMDALTATRTYQTIIELFIQQCPFFFYNRPITEPPGARPSSPSAVASTVPFLTSTPVTS QPSPPQSPPPTPQSPMQPLLPSPLHPHRPTPQPSRNIPWQTKEH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004491 |
| ORF Size | 4545 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004491.2, NP_004482.2 |
| RefSeq Size | 4545 bp |
| RefSeq ORF | 4500 bp |
| Locus ID | 2909 |
| UniProt ID | Q9NRY4 |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton |
| Gene Summary | The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222277 | ARHGAP35 (Myc-DDK-tagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1) |
CNY 10,360.00 |
|
| RC222277L3 | Lenti-ORF clone of ARHGAP35 (Myc-DDK-tagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1) |
CNY 12,730.00 |
|
| RC222277L4 | Lenti-ORF clone of ARHGAP35 (mGFP-tagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1) |
CNY 12,730.00 |
|
| SC308989 | ARHGAP35 (untagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1) |
CNY 28,880.00 |
