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DIAPH3 (NM_030932) Human Untagged Clone
CAT＃: SC305300
DIAPH3 (untagged)-Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305300 representing NM_030932.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTGAGGAGAGGAGCCTTTCCTTATTGGCCAAAGCCGTGGATCCCAGACACCCCAATATGATGACA GATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATCCTTGAAGAAGTTTTAGAA GCTTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTTTTTTGTATTGTGGAAGGCCTCCGGCAC AATTCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTACATCTCCTGATGATTTG GATTTCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAATTTAAAA TGCATTAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGAAGAAGATTTGTTT GAGTTATCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATGTTTACAACATGGTG TGGAGCACAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCATCTTTTGCTGATT CGAAATGATTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAGTGTGTATCCCAGATTGTATTG CATAGAGATGGAATGGATCCAGACTTCACATATCGAAAAAGACTAGATTTAGATTTAACCCAGTTTGTA GACATTTGCATAGATCAAGCAAAACTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTACAAGAAATTT GAAAAAGAGTTTACCGACCACCAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGATTAATGAG CTTCAAGCAGAGCTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATTCCTTTG CCTCCCTCTAAAGAAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCTGGTGGA GGGGTGCCGCCTCCACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGGATGCCATTCAGTGGT CCTGTGCCTCCACCACCTCCCCTGGGATTCCTTGGAGGACAAAATTCTCCTCCTCTACCAATCCTGCCA TTTGGGTTGAAACCAAAGAAAGAATTTAAACCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATC AGACCTCATGAAATGACTGAAAACTGTTTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGAT TTGCTTTGTAAACTTGAGAATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAA GAGAAGAAATCGATTAAGAAAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTT TCAATCTTCCTGAGCTCTTTTCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAAGTAGATGAA ACACGGTTGGCAGAGTCTATGATTCAGAACTTAATAAAGCATCTTCCTGATCAAGAGCAATTAAATTCA TTGTCTCAGTTCAAGAGTGAATATAGCAACTTATGTGAACCTGAGCAGTTTGTGGTTGTGATGAGCAAT GTGAAGAGACTACGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAAC ATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAG TTGCTGGAACTTGTATTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGA TTTAACCTTAGCTCTCTCTGTAAACTAAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTTCAT TTCCTGGTAGAAATATGTGAAGAGAAGTACCCTGATATACTGAATTTTGTGGATGATTTGGAACCTTTA GACAAAGCTAGTAAAGTCTCTGTAGAAACGCTGGAAAAGAATTTGAGGCAGATGGGAAGGCAGCTTCAA CAGCTTGAGAAGGAATTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGACAAAGATG TCCAGATTTGTTATCAGTGCAAAAGAACAATATGAGACACTTTCGAAGTTACACGAAAACATGGAAAAG TTATACCAGAGTATAATAGGATACTATGCCATTGATGTGAAGAAGGTGTCTGTGGAAGACTTTCTTACT GACCTGAATAACTTCAGAACCACATTCATGCAAGCAATAAAGGAGAATATCAAAAAAAGAGAAGCAGAG GAAAAAGAAAAACGTGTCAGAATAGCTAAAGAATTAGCAGAGCGAGAAAGACTCGAACGCCAACAAAAG AAAAAGCGTTTATTAGAAATGAAGACTGAGGGTGATGAGACAGGAGTGATGGATAATCTGCTGGAGGCC TTGCAGTCCGGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCGATGCCAAAAGATGTTCGGCAGAGT CTCAGTCCAATGTCTCAGAGGCCTGTTCTGAAAGTTTGTAACCATGGTAATAAACCGTATTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_030932 |
| Insert Size | 2550 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030932.3 |
| RefSeq Size | 2745 bp |
| RefSeq ORF | 2550 bp |
| Locus ID | 81624 |
| UniProt ID | Q9NSV4 |
| Protein Pathways | Regulation of actin cytoskeleton |
| MW | 98.1 kDa |
| Gene Summary | This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (2) differs in the 5' and 3' UTR, lacks a portion of the 5' and 3' coding regions, and initiates translation at an alternate start codon compared to variant 1. The resulting protein (isoform b) has shorter N- and C- termini compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205246 | DIAPH3 (Myc-DDK-tagged)-Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2 |
CNY 6,216.00 |
|
| RC205246L3 | Lenti ORF clone of Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC205246L4 | Lenti ORF clone of Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RG205246 | DIAPH3 (tGFP-tagged) - Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2 |
CNY 7,816.00 |
