DIAPH3 (NM_030932) Human Tagged ORF Clone
CAT#: RG205246
- TrueORF®
DIAPH3 (tGFP-tagged) - Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2
ORF Plasmid: DDK
"NM_030932" in other vectors (4)
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CNY 7,816.00
CNY 7,890.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205246 representing NM_030932
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGGAGAGGAGCCTTTCCTTATTGGTCAAAGCCGTGGATCCCAGACACCCCAATATGATGACAG ATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATCCTTGAAGAAGTTTTAGAAGC TTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTTTTTTGTATTGTGGAAGGCCTCCGGCACAAT TCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTACATCTCCTGATGATTTGGATT TCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAATTTAAAATGCAT TAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGAAGAAGATTTGTTTGAGTTA TCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATGTTTACAACATGGTGTGGAGCA CAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCATCTGTTGCTGATTCGAAATGA TTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAGTGTGTATCCCAGATTGTATTGCATAGAGAT GGAATGGATCCAGACTTCACATATCGAAAAAGACTAGATTTAGATTTAACCCAGTTTGTAGACATTTGCA TAGATCAAGCAAAACTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTACAAGAAATTTGAAAAAGAGTT TACCGACCACCAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGATTAATGAGCTTCAAGCAGAG CTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATTCCTTTGCCTCCCTCTAAAG AAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCTGGTGGAGGGGTGCCGCCTCC ACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGGATGCCATTCAGTGGTCCTGTGCCTCCACCA CCTCCCCTGGGATTCCTTGGAGGACAAAATTCTCCTCCTCTACCAATCCTGCCATTTGGGTTGAAACCAA AGAAAGAATTTAAACCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCAGACCTCATGAAATGAC TGAAAACTGTTTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGATTTGCTTTGTAAACTTGAG AATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAAGAGAAGAAATCGATTAAGA AAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTTTCAATCTTCCTGAGCTCTTT TCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAAGTAGATGAAACACGGTTGGCAGAGTCTATG ATTCAGAACTTAATAAAGCATCTTCCTGATCAAGAGCAATTAAATTCATTGTCTCAGTTCAAGAGTGAAT ATAGCAACTTATGTGAACCTGAGCAGTTTGTGGTTGTGATGAGCAATGTGAAGAGACTACGGCCACGGCT CAGTGCTATTCTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTC AGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAGTTGCTGGAACTTGTATTGCTAATGG GAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTAACCTTAGCTCTCTCTGTAAACT AAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTTCATTTCCTGGTAGAAATATGTGAAGAGAAG TACCCTGATATACTGAATTTTGTGGATGATTTGGAACCTTTAGACAAAGCTAGTAAAGTCTCTGTAGAAA CGCTGGAAAAGAATTTGAGGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGGAATTGGAAACCTTTCC CCCTCCTGAGGACTTGCATGACAAGTTTGTGACAAAGATGTCCAGATTTGTTATCAGTGCAAAAGAACAA TATGAGACACTTTCGAAGTTACACGAAAACATGGAAAAGTTATACCAGAGTATAATAGGATACTATGCCA TTGATGTGAAGAAGGTGTCTGTGGAAGACTTTCTTACTGACCTGAATAACTTCAGAACCACATTCATGCA AGCAATAAAGGAGAATATCAAAAAAAGAGAAGCAGAGGAAAAAGAAAAACGTGTCAGAATAGCTAAAGAA TTAGCAGAGCGAGAAAGACTCGAACGCCAACAAAAGAAAAAGCGTTTATTAGAAATGAAGACTGAGGGTG ATGAGACAGGAGTGATGGATAATCTGCTGGAGGCCTTGCAGTCCGGGGCTGCCTTCCGCGACAGAAGAAA AAGGACACCGATGCCAAAAGATGTTCGGCAGAGTCTCAGTCCAATGTCTCAGAGGCCTGTTCTGAAAGTT TGTAACCATGGTAATAAACCGTATTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205246 representing NM_030932
Red=Cloning site Green=Tags(s) MSEERSLSLLVKAVDPRHPNMMTDVVKLLSAVCIVGEESILEEVLEALTSAGEEKKIDRFFCIVEGLRHN SVQLQVACMQLINALVTSPDDLDFRLHIRNEFMRCGLKEILPNLKCIKNDGLDIQLKVFDEHKEEDLFEL SHRLEDIRAELDEAYDVYNMVWSTVKETRAEGYFISILQHLLLIRNDYFIRQQYFKLIDECVSQIVLHRD GMDPDFTYRKRLDLDLTQFVDICIDQAKLEEFEEKASELYKKFEKEFTDHQETQAELQKKEAKINELQAE LQAFKSQFGALPADCNIPLPPSKEGGTGHSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPFSGPVPPP PPLGFLGGQNSPPLPILPFGLKPKKEFKPEISMRRLNWLKIRPHEMTENCFWIKVNENKYENVDLLCKLE NTFCCQQKERREEEDIEEKKSIKKKIKELKFLDSKIAQNLSIFLSSFRVPYEEIRMMILEVDETRLAESM IQNLIKHLPDQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAV STACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEK YPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQ YETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKE LAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKV CNHGNKPYL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030932 |
ORF Size | 2547 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030932.3, NP_112194.2 |
RefSeq Size | 2745 bp |
RefSeq ORF | 2550 bp |
Locus ID | 81624 |
UniProt ID | Q9NSV4 |
Protein Pathways | Regulation of actin cytoskeleton |
Gene Summary | This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205246 | DIAPH3 (Myc-DDK-tagged)-Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2 |
CNY 6,216.00 |
|
RC205246L3 | Lenti ORF clone of Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
RC205246L4 | Lenti ORF clone of Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
SC305300 | DIAPH3 (untagged)-Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2 |
CNY 7,220.00 |