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DNASE2B (NM_021233) Human Untagged Clone
CAT＃: SC304928
DNASE2B (untagged)-Human deoxyribonuclease II beta (DNASE2B), transcript variant 1
CN¥ 7,220.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DLAD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304928 representing NM_021233.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAACAGAAAATGATGGCAAGACTGCTAAGAACATCCTTTGCTTTGCTCTTCCTTGGCCTCTTTGGG GTGCTGGGGGCAGCAACAATTTCATGCAGAAATGAAGAAGGGAAAGCTGTGGACTGGTTTACTTTTTAT AAGTTACCTAAAAGACAAAACAAGGAAAGTGGAGAGACTGGGTTAGAGTACCTGTACCTAGACTCTACA ACTAGAAGCTGGAGGAAGAGTGAGCAACTAATGAATGACACCAAGAGTGTTTTGGGAAGGACATTACAA CAGCTATATGAAGCATATGCCTCTAAGAGTAACAACACAGCCTATCTAATATACAATGATGGAGTCCCT AAACCTGTGAATTACAGCAGAAAGTATGGACACACCAAAGGTTTACTGCTGTGGAACAGAGTTCAAGGG TTCTGGCTGATTCATTCCATCCCTCAGTTTCCTCCAATTCCGGAAGAAGGCTATGATTATCCACCCACA GGGAGACGAAATGGACAAAGTGGCATCTGCATAACTTTCAAGTACAACCAGTATGAGGCAATAGATTCT CAGCTCTTGGTCTGCAACCCCAACGTCTATAGCTGCTCCATCCCAGCCACCTTTCACCAGGAGCTCATT CACATGCCCCAGCTGTGCACCAGGGCCAGCTCATCAGAGATTCCTGGCAGGCTCCTCACCACACTTCAG TCGGCCCAGGGACAAAAATTCCTCCATTTTGCAAAGTCGGATTCTTTTCTTGACGACATCTTTGCAGCC TGGATGGCTCAACGGCTGAAGACACACTTGTTAACAGAAACCTGGCAGCGAAAAAGACAAGAGCTTCCT TCAAACTGCTCCCTTCCTTACCATGTCTACAATATAAAAGCAATTAAATTATCACGACACTCTTATTTC AGTTCTTATCAAGATCATGCCAAGTGGTGTATTTCCCAAAAGGGCACCAAAAATCGCTGGACATGTATT GGAGACCTAAATCGGAGTCCACACCAAGCCTTCAGAAGTGGAGGATTCATTTGTACCCAGAATTGGCAA ATTTACCAAGCATTTCAAGGATTAGTATTATACTATGAAAGCTGTAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_021233 |
| Insert Size | 1086 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021233.2 |
| RefSeq Size | 1279 bp |
| RefSeq ORF | 1086 bp |
| Locus ID | 58511 |
| UniProt ID | Q8WZ79 |
| Protein Families | Transmembrane |
| Protein Pathways | Lysosome |
| MW | 41.7 kDa |
| Gene Summary | The protein encoded by this gene shares considerable sequence similarity to, and is structurally related to DNase II. The latter is a well characterized endonuclease that catalyzes DNA hydrolysis in the absence of divalent cations at acidic pH. Unlike DNase II which is ubiquitously expressed, expression of this gene product is restricted to the salivary gland and lungs. The gene has been localized to chromosome 1p22.3 adjacent (and in opposite orientation) to the uricase pseudogene. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the full-length transcript and encodes the longer isoform (1), which is more highly expressed in the salivary gland. |
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Other Versions
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| RC212192 | DNASE2B (Myc-DDK-tagged)-Human deoxyribonuclease II beta (DNASE2B), transcript variant 1 |
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| RC212192L2 | Lenti ORF clone of Human deoxyribonuclease II beta (DNASE2B), transcript variant 1, mGFP tagged |
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| RC212192L3 | Lenti ORF clone of Human deoxyribonuclease II beta (DNASE2B), transcript variant 1, Myc-DDK-tagged |
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| RC212192L4 | Lenti ORF clone of Human deoxyribonuclease II beta (DNASE2B), transcript variant 1, mGFP tagged |
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| RG212192 | DNASE2B (tGFP-tagged) - Human deoxyribonuclease II beta (DNASE2B), transcript variant 1 |
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