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REV1 (NM_001037872) Human Untagged Clone
CAT＃: SC302943
REV1 (untagged)-Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 2
CNY 10,450.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AIBP80; REV1L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302943 representing NM_001037872.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGCGAGGTGGATGGAGGAAGCGAGCTGAAAATGATGGCTGGGAAACATGGGGTGGGTATATGGCT GCCAAGGTCCAGAAATTGGAGGAACAGTTTCGATCAGATGCTGCTATGCAGAAGGATGGGACTTCATCT ACAATTTTTAGTGGAGTTGCCATCTATGTTAATGGATACACAGATCCTTCCGCTGAGGAATTGAGAAAA CTAATGATGTTGCATGGAGGTCAATACCATGTATATTATTCCAGATCTAAAACAACACATATTATTGCC ACAAATCTTCCCAATGCCAAAATTAAAGAATTAAAGGGGGAAAAAGTAATTCGACCAGAATGGATTGTG GAAAGCATCAAAGCTGGACGACTCCTCTCCTACATTCCATATCAGCTGTACACCAAGCAGTCCAGTGTG CAGAAAGGTCTCAGCTTTAATCCTGTATGCAGACCTGAGGATCCTCTGCCAGGTCCAAGCAATATAGCC AAACAGCTCAACAACAGGGTAAATCACATCGTTAAGAAGATTGAAACGGAAAATGAAGTCAAAGTCAAT GGCATGAACAGTTGGAATGAAGAAGATGAAAATAATGATTTTAGTTTTGTGGATCTGGAGCAGACCTCT CCGGGAAGGAAACAGAATGGAATTCCGCATCCCAGAGGGAGCACTGCCATTTTTAATGGACACACTCCT AGCTCTAATGGTGCCTTAAAGACACAGGATTGCTTGGTGCCCATGGTCAACAGTGTTGCCAGCAGGCTT TCTCCAGCCTTTTCCCAGGAGGAGGATAAGGCTGAGAAGAGCAGCACTGATTTCAGAGACTGCACTCTG CAGCAGTTGCAGCAAAGCACCAGAAACACAGATGCTTTGCGGAATCCACACAGAACTAATTCTTTCTCA TTATCACCTTTGCACAGTAACACTAAAATCAATGGTGCTCACCACTCCACTGTTCAGGGGCCTTCAAGC ACAAAAAGCACTTCTTCAGTATCTACGTTTAGCAAGGCAGCACCTTCAGTGCCATCCAAACCTTCAGAC TGCAATTTTATTTCAAACTTCTATTCTCATTCAAGACTGCATCACATATCAATGTGGAAGTGTGAATTG ACTGAGTTTGTCAATACCCTACAAAGACAAAGTAATGGTATCTTTCCAGGAAGGGAAAAGTTAAAAAAA ATGAAAACAGGCAGGTCTGCACTTGTTGTAACTGACACAGGAGATATGTCAGTATTGAATTCTCCCAGA CATCAGAGCTGTATAATGCATGTTGATATGGATTGCTTCTTTGTATCAGTGGGTATACGAAATAGACCA GATCTCAAAGGAAAACCAGTGGCTGTTACAAGTAACAGAGGCACAGGAAGGGCACCTTTACGTCCTGGC GCTAACCCCCAGCTGGAGTGGCAGTATTACCAGAATAAAATCCTGAAAGGCAAAGCAGATATACCAGAT TCATCATTGTGGGAGAATCCAGATTCTGCGCAAGCAAATGGAATTGATTCTGTTTTGTCAAGGGCTGAA ATTGCATCTTGTAGTTATGAGGCCAGGCAACTTGGCATTAAGAACGGAATGTTTTTTGGGCATGCTAAA CAACTATGTCCTAATCTTCAAGCTGTTCCATACGATTTTCATGCATATAAGGAAGTCGCACAAACATTG TATGAAACATTGGCAAGCTACACTCATAACATTGAAGCTGTCAGTTGTGATGAAGCGCTGGTAGACATT ACCGAAATCCTTGCAGAGACCAAACTTACTCCTGATGAATTTGCAAATGCTGTTCGTATGGAAATCAAA GACCAGACGAAATGTGCTGCCTCTGTTGGAATTGGTTCTAATATTCTCCTGGCTAGAATGGCAACTAGA AAAGCAAAACCAGATGGGCAGTACCACCTAAAACCAGAAGAAGTAGATGATTTTATCAGAGGCCAGCTA GTGACCAATCTACCAGGAGTTGGACATTCAATGGAATCTAAGTTGGCATCTTTGGGAATTAAAACTTGT GGAGACTTGCAGTATATGACCATGGCAAAACTCCAAAAAGAATTTGGTCCCAAAACAGGTCAGATGCTT TATAGGTTCTGCCGTGGCTTGGATGATAGACCAGTTCGAACTGAAAAGGAAAGAAAATCTGTTTCAGCT GAGATCAACTATGGAATAAGGTTTACTCAGCCAAAAGAGGCAGAAGCTTTTCTTCTGAGTCTTTCAGAA GAAATTCAAAGAAGACTAGAAGCCACTGGCATGAAGGGTAAACGTCTAACTCTCAAAATCATGGTACGA AAGCCTGGGGCTCCTGTAGAAACTGCAAAATTTGGAGGCCATGGAATTTGTGATAACATTGCCAGGACT GTAACTCTTGACCAGGCAACAGATAATGCAAAAATAATTGGAAAGGCGATGCTAAACATGTTTCATACA ATGAAACTAAATATATCAGATATGAGAGGGGTTGGGATTCACGTGAATCAGTTGGTTCCAACTAATCTG AACCCTTCCACATGTCCCAGTCGCCCATCAGTTCAGTCAAGCCACTTTCCTAGTGGGTCATACTCTGTC CGTGATGTCTTCCAAGTTCAGAAAGCTAAGAAATCCACCGAAGAGGAGCACAAAGAAGTATTTCGGGCT GCTGTGGATCTGGAAATATCATCTGCTTCTAGAACTTGCACTTTCTTGCCACCTTTTCCTGCACATCTG CCGACCAGTCCTGATACTAACAAGGCTGAGTCTTCAGGGAAATGGAATGGTCTACATACTCCTGTCAGT GTGCAGTCGAGACTTAACCTGAGTATAGAGGTCCCGTCACCTTCCCAGCTGGATCAGTCTGTTTTAGAA GCACTTCCACCTGATCTCCGGGAACAAGTAGAGCAAGTCTGTGCTGTCCAGCAAGCAGAGTCACATGGC GACAAAAAGAAAGAACCAGTAAATGGCTGTAATACAGGAATTTTGCCACAACCAGTTGGGACAGTCTTG TTGCAAATACCAGAACCTCAAGAATCGAACAGTGACGCAGGAATAAATTTAATAGCCCTTCCAGCATTT TCACAGGTGGACCCTGAGGTATTTGCTGCCCTTCCTGCTGAACTTCAGAGGGAGCTGAAAGCAGCGTAT GATCAAAGACAAAGGCAGGGCGAGAACAGCACTCACCAGCAGTCAGCCAGCGCATCTGTGCCAAAGAAT CCTTTACTTCATCTAAAGGCAGCAGTGAAAGAAAAGAAAAGAAACAAGAAGAAAAAAACCATTGGTTCA CCAAAAAGGATTCAGAGTCCTTTGAATAACAAGCTGCTTAACAGTCCTGCAAAAACTCTGCCAGGGGCC TGTGGCAGTCCCCAGAAGTTAATTGATGGGTTTCTAAAACATGAAGGACCTCCTGCAGAGAAACCCCTG GAAGAACTCTCTGCTTCTACTTCAGGTGTGCCAGGCCTTTCTAGTTTGCAGTCTGACCCAGCTGGCTGT GTGAGACCTCCAGCACCCAATCTAGCTGGAGCTGTTGAATTCAATGATGTGAAGACCTTGCTCAGAGAA TGGATAACTACAATTTCAGATCCAATGGAAGAAGACATTCTCCAAGTTGTGAAATACTGTACTGATCTA ATAGAAGAAAAAGATTTGGAAAAACTGGATCTAGTTATAAAATACATGAAAAGGCTGATGCAGCAATCG GTGGAATCGGTTTGGAATATGGCATTTGACTTTATTCTTGACAATGTCCAGGTGGTTTTACAACAAACT TATGGAAGCACATTAAAAGTTACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001037872 |
| Insert Size | 3753 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001037872.2 |
| RefSeq Size | 4786 bp |
| RefSeq ORF | 3753 bp |
| Locus ID | 51455 |
| UniProt ID | Q9UBZ9 |
| Protein Families | Druggable Genome |
| MW | 138.2 kDa |
| Gene Summary | This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Vital Role of Polymerase and REV1 in Mutagenic, but Not Correct, DNA Synthesis across Benzo[a]pyrene-dG and Recruitment of Polymerase by REV1 to Replication-stalled Site
,Keiji Hashimoto, Youngjin Cho, In-Young Yang, Jun-ichi Akagi, Eiji Ohashi, Satoshi Tateishi, Niels de Wind, Fumio Hanaoka, Haruo Ohmori, and Masaaki Moriya,
J. Biol. Chem., Mar 2012; 287: 9613 - 9622.
[REV1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217528 | REV1 (Myc-DDK-tagged)-Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 2 |
CNY 8,704.00 |
|
| RC217528L3 | Lenti ORF clone of Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 2, Myc-DDK-tagged |
CNY 14,350.00 |
|
| RC217528L4 | Lenti ORF clone of Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 2, mGFP tagged |
CNY 14,350.00 |
|
| RG217528 | REV1 (tGFP-tagged) - Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 2 |
CNY 13,680.00 |
