Ankyrin brain (ANK2) (NM_020977) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANK-2; brank-2; CFAP87; FAP87; LQT4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_020977, the custom clone sequence may differ by one or more nucleotides ATGATGAACGAAGATGCAGCTCAGAAAAGCGACAGTGGAGAGAAGTTCAACGGCAGTAGTCAGAGGAGAA AAAGACCCAAGAAGTCTGACAGCAATGCAAGCTTCCTCCGTGCTGCCAGAGCAGGCAACCTGGACAAAGT TGTGGAATATCTGAAGGGGGGCATAGACATCAATACCTGCAATCAGAATGGACTCAACGCTCTCCATCTG GCTGCCAAGGAAGGCCACGTGGGGCTGGTGCAGGAGCTGCTGGGAAGAGGGTCCTCTGTGGATTCTGCCA CTAAGAAGGGAAATACCGCTCTTCACATTGCATCTTTGGCTGGACAAGCAGAAGTTGTCAAAGTTCTTGT TAAGGAAGGAGCCAATATTAATGCACAGTCTCAGAATGGCTTTACTCCTTTATACATGGCTGCCCAAGAG AATCACATTGATGTTGTAAAATATTTGCTGGAAAATGGAGCTAATCAGAGCACTGCTACAGAGGATGGCT TTACTCCTCTAGCTGTGGCACTCCAGCAAGGACACAACCAGGCGGTGGCCATCCTCTTGGAGAATGACAC CAAAGGGAAAGTGAGGCTGCCAGCTCTGCATATTGCCGCTAGGAAAGACGACACCAAATCTGCCGCACTT CTGCTTCAGAATGACCACAATGCTGACGTACAATCCAAGATGATGGTGAATAGGACAACTGAGAGTGGTT TTACCCCTTTGCACATAGCTGCACATTACGGAAATGTCAACGTGGCAACTCTTCTTCTAAACCGGGGAGC TGCTGTGGACTTCACAGCCAGGAATGGAATCACTCCTCTGCATGTGGCTTCCAAAAGAGGAAATACAAAC ATGGTGAAGCTCTTACTGGATCGAGGCGGTCAGATCGATGCCAAAACTAGGGATGGGTTGACACCACTTC ACTGTGCTGCACGAAGTGGGCATGACCAAGTGGTGGAACTTCTGTTGGAACGGGGTGCCCCCTTGCTGGC AAGGACTAAGAATGGGCTGTCTCCACTACACATGGCTGCCCAGGGAGACCACGTGGAATGTGTGAAGCAC CTGTTACAGCACAAGGCACCTGTTGATGATGTCACCCTAGACTACCTGACAGCCCTCCACGTTGCTGCGC ACTGTGGCCACTACCGTGTAACCAAACTCCTTTTAGACAAGAGAGCCAATCCGAACGCCAGAGCCCTGAA TGGTTTTACTCCACTGCACATTGCCTGCAAGAAAAACCGCATCAAAGTCATGGAACTGCTGGTGAAATAT GGGGCTTCAATCCAAGCTATAACAGAGTCTGGCCTCACACCAATACATGTGGCTGCCTTCATGGGCCACT TGAACATTGTCCTCCTTCTGCTGCAGAACGGAGCCTCTCCAGATGTCACTAACATTCGTGGTGAGACGGC ACTACACATGGCAGCCCGAGCCGGGCAGGTGGAAGTGGTCCGATGCCTCCTGAGAAATGGTGCCCTTGTT GATGCCAGAGCCAGGGAGGAACAGACACCTTTACATATTGCCTCCCGCCTGGGTAAGACAGAAATTGTCC AGCTGCTTCTACAACATATGGCTCATCCAGATGCGGCCACTACAAATGGGTACACACCACTGCACATCTC TGCCCGGGAGGGCCAGGTGGATGTGGCATCAGTCCTATTGGAAGCAGGAGCAGCCCACTCCTTAGCTACC AAGAAGGGTTTTACTCCCCTGCATGTAGCAGCCAAGTATGGAAGCCTGGATGTGGCAAAACTTCTCTTGC AACGCCGTGCTGCCGCAGATTCTGCAGGGAAGAACGGCCTTACCCCGCTCCATGTTGCTGCTCATTATGA CAACCAGAAGGTGGCGCTGCTGTTACTGGAGAAGGGTGCTTCCCCTCATGCCACTGCCAAGAATGGCTAT ACTCCGTTACATATTGCTGCCAAGAAGAATCAAATGCAGATAGCTTCCACACTCCTGAACTATGGAGCAG AGACAAACATTGTGACAAAGCAAGGAGTAACTCCACTCCATCTGGCCTCGCAGGAGGGGCACACAGATAT GGTTACCTTGCTTCTGGATAAGGGAGCCAATATCCACATGTCAACTAAGAGTGGACTCACATCCTTACAC CTTGCAGCCCAGGAAGATAAAGTGAATGTTGCTGATATTCTCACCAAGCATGGAGCTGATCAGGATGCTC ATACAAAGCTTGGTTACACACCTTTAATTGTGGCCTGTCACTATGGAAATGTGAAAATGGTCAACTTTCT TCTGAAGCAGGGAGCAAATGTTAACGCAAAAACCAAGAACGGCTACACGCCTTTGCACCAGGCCGCTCAG CAGGGTCACACGCACATCATCAACGTCCTGCTCCAGCATGGGGCCAAGCCCAACGCCACCACTGCGAATG GCAACACTGCCTTGGCGATTGCTAAGCGTCTGGGCTACATCTCCGTGGTCGACACCCTGAAGGTTGTGAC TGAGGAGGTCACCACCACCACCACAACTATTACAGAAAAACACAAACTAAATGTACCTGAGACGATGACT GAGGTTCTTGATGTTTCTGATGAAGAGGGTGATGACACAATGACTGGTGATGGGGGAGAATACCTTAGGC CTGAGGACCTAAAAGAACTGGGTGATGACTCACTACCCAGCAGTCAGTTCCTGGATGGTATGAATTACCT GCGATACAGCTTGGAGGGAGGACGATCTGACAGCCTTCGATCCTTCAGTTCCGACAGGTCTCACACTCTG AGCCATGCCTCCTACCTGAGGGACAGTGCCGTGATGGATGACTCAGTTGTGATTCCCAGTCACCAGGTGT CAACTCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGCCTAAGCTGGGGCACTGAGAACTTAGACAACGT GGCTCTTTCTTCTAGTCCTATTCATTCAGGTTTCCTGGTTAGTTTTATGGTGGATGCCCGAGGTGGTGCT ATGCGAGGATGCAGACACAATGGGCTCCGAATCATTATTCCACCTCGGAAATGTACTGCTCCAACGCGAG TCACCTGCCGACTGGTCAAGCGCCACAGACTGGCAACAATGCCTCCAATGGTGGAAGGAGAAGGCCTGGC CAGTCGCCTGATCGAAGTTGGACCTTCTGGTGCTCAGTTCCTTGGTAAACTTCACCTGCCAACGGCTCCT CCCCCACTTAATGAGGGAGAAAGTTTGGTCAGCCGCATTCTTCAGCTGGGGCCTCCTGGAACCAAATTCC TTGGGCCTGTGATCGTGGAGATCCCTCACTTTGCGGCCCTTCGAGGAAAGGAAAGGGAACTGGTGGTCCT GCGCAGTGAGAATGGGGACAGCTGGAAAGAGCATTTCTGTGACTACACTGAAGATGAATTGAATGAAATT CTTAACGGCATGGATGAAGTACTGGATAGCCCAGAAGACCTAGAAAAGAAACGAATCTGCCGCATCATCA CCCGAGACTTCCCACAGTACTTTGCAGTGGTGTCTCGTATCAAACAGGACAGCAATCTGATTGGCCCAGA AGGAGGTGTACTGAGCAGCACAGTGGTGCCCCAGGTGCAGGCCGTCTTCCCAGAGGGGGCACTCACCAAG CGGATCCGCGTAGGCCTGCAGGCTCAACCTATGCACAGTGAGCTGGTTAAGAAGATCCTAGGCAACAAAG CTACCTTCAGCCCTATAGTCACTTTGGAACCTAGAAGAAGAAAATTCCACAAACCAATTACCATGACCAT TCCTGTCCCCAAAGCTTCAAGTGATGTCATGTTGAATGGTTTTGGGGGAGATGCACCAACCTTAAGATTA CTATGCAGCATAACAGGTGGAACCACCCCTGCCCAGTGGGAAGATATTACAGGAACTACGCCATTAACAT TTGTCAATGAATGTGTTTCCTTTACAACAAACGTGTCTGCCAGGTTCTGGCTGATAGATTGTCGACAGAT CCAGGAATCCGTTACTTTTGCATCACAAGTATACAGAGAAATTATCTGCGTACCTTATATGGCCAAATTT GTAGTGTTTGCCAAATCACATGACCCCATTGAAGCCAGGTTGAGGTGTTTCTGCATGACTGATGATAAAG TGGATAAGACCCTTGAACAACAAGAAAATTTTGCTGAGGTGGCCAGAAGCAGGGATGTGGAGGTGTTAGA AGGAAAACCCATCTACGTTGATTGTTTCGGCAACTTGGTACCATTAACTAAAAGTGGCCAGCATCATATA TTCAGTTTTTTTGCCTTCAAAGAAAATAGACTTCCTCTATTTGTCAAGGTACGCGATACGACTCAGGAAC CTTGCGGACGACTATCATTTATGAAGGAGCCAAAATCCACGAGAGGCCTGGTGCATCAAGCTATTTGCAA CTTAAACATCACTTTGCCGATTTATACAAAGGAATCAGAGTCAGATCAAGAACAGGAGGAAGAGATCGAT ATGACATCAGAAAAAAATCCACAGGATGAGCAGGAACGGATCGAGGAAAGGCTGGCTTATATTGCTGATC ACCTTGGCTTCAGCTGGACAGAATTAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCATCAAATTCG AATTGAAAATCCCAACTCTCTTCAAGACCAGAGTCATGCACTGTTGAAGTACTGGCTAGAGAGGGATGGG AAACATGCTACAGATACCAACCTCGTTGAATGTCTCACCAAGATCAACCGAATGGATATTGTTCATCTCA TGGAGACCAACACAGAACCTCTCCAGGAGCGCATCAGTCATAGTTATGCAGAAATTGAACAGACCATTAC ACTGGATCATAGTGAAGGGTTCTCGGTACTTCAAGAGGAGTTATGCACTGCACAGCACAAGCAGAAAGAG GAGCAAGCTGTTTCTAAAGAAAGTGAGACCTGCGATCACCCTCCTATCGTCTCAGAGGAAGACATTTCTG TTGGTTATTCCACTTTTCAGGATGGCGTCCCCAAAACTGAGGGGGACAGCTCAGCAACAGCACTCTTTCC CCAAACTCACAAGGAGCAAGTTCAACAGGATTTCTCAGGGAAAATGCAAGACCTGCCTGAAGAGTCATCT CTGGAATATCAGCAGGAATATTTTGTGACAACTCCAGGAACAGAAACATCAGAGACTCAGAAGGCTATGA TAGTACCCAGCTCTCCCAGCAAGACACCTGAGGAAGTTAGCACCCCTGCAGAGGAGGAGAAGCTGTACCT CCAGACCCCAACATCCAGCGAGCGGGGAGGCTCTCCCATCATACAAGAACCCGAAGAGCCCTCAGAGCAC AGAGAGGAGAGCTCTCCGCGGAAAACCAGCCTCGTAATAGTGGAGTCTGCCGATAACCAGCCTGAGACCT GTGAAAGACTCGATGAAGATGCAGCTTTTGAAAAGGGAGACGATATGCCTGAAATACCCCCAGAAACAGT CACAGAAGAAGAATACATTGATGAGCATGGACACACCGTGGTAAAGAAGGTTACTAGGAAAATCATTAGG CGGTATGTATCCTCTGAAGGCACAGAGAAAGAAGAGATTATGGTGCAGGGAATGCCACAGGAACCTGTCA ACATCGAGGAAGGGGATGGCTATTCCAAAGTTATAAAGCGTGTTGTATTGAAGAGTGACACCGAGCAGTC AGAGGACAACAATGAGTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_020977 unedited AGCTTTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTTTGATGGCAG AGATATTTTCTTTCCAAACTGTTCAAAATGATGAACGAAGATGCAGCTCAGAAAAGCGAC AGTGGAGAGAAGTTCAACGGCAGTAGTCAGAGGAGAAAAAGACCCAAGAAGTCTGACAGC AATGCAAGCTTCCTCCGTGCTGCCAGAGCAGGCAACCTGGACAAAGTTGTGGAATATCTG AAGGGGGGCATAGACATCAATACCTGCAATCAGAATGGACTCAACGCTCTCCATCTGGCT GCCAAGGAAGGCCACGTGGGGCTGGTGCAGGAGCTGCTGGGAAGAGGGTCCTCTGTGGAT TCTGCCACTAAGAAGGGAAATACCGCTCTTCACATTGCATCTTTGGCTGGACAAGCAGAA GTTGTCAAAGTTCTTGTTAAGGAAGGAGCCAATATTAATGCACAGTCTCAGAATGGCTTT ACTCCTTTATACATGGCTGCCCAAGAGAATCACATTGATGTTGTAAAATATTTGCTGGAA AATGGAGCTAATCAGAGCACTGCTACAGAGGATGGCTTTACTCCTCTAGCTGTGGCACTC CAGCAAGGACACAACCAGGCGGTGGCCATCCTCTTGGAGAATGACACCAAAGGGAAAGTG AGGCTGCCAGCTCTGCATATTGCCGCTAGGAAAGACGACACCAAATCTGCCGCACTTCTG CTTCAGAATGACCACAATGCTGACGTACAATCCAAGAGTGGTTTTACCCCTTTGCACATA GCTGCACATTACGGGAATGTCAACGTGGCAACTCTTCTTCTTAACCGGGAGCTGCTGTGG ACTCACAGCCAGGATGGGATCACTCCTCTGCATGTGGCTTCAAAGAAGAATACAACATGT GAGCTCNACTGGATCGAGCGTCAGACGTGCCAACTAGGNATGGTGACCCATT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_020977 unedited GCCGCCTACAGGCNAAAGCCGTCTTTTTTCCCCCCCCCCCGGGTACGGNCTNAAAAACCC AATTTTACGGCCTTGNACACATCGCAGCTATGTATTGTTAGTGTTGTTTTTTTTTTTCCA TTAACTAATACATGCCCTCATAGATATATTCAATTAGTGTTATCACCATGGGAACAAGAT GCTGATTCGTCAACTGGAAAATTCACTTCTTCTCACAGTATTCAAGTTTTTGATAACACA GCAAAAATCAAAATCAAAAATTGTTTAATAACCATTATGTTGTTACACAGACATCATCTG CACTGCAAATAACACAACAGAAATGCTTTTCTTGAGCTCCAGTGAAGGTTTTGACAAGAA GGCCATAGGGTGTCTCAAAAAGTTTCACCATCGGCCTCTCTTATTTTGGCTGTCAAAGAA TGTCTTATTTCCTATCCAACTGCCAAAAAGTGCTAGCATTTGAGGCTTAGGAGATTTGGC TTGTGGGAAACCTCCCTCCCAGGAAATTTTTTCTCTTGGCAACATTACCTTAAATGATGA AGTGCCATTGCTTGCCGCATTTGTTTTTGACATTCGGAGCAATGAGTTATTTCTGCCCAC CAGGAAAAGACCACATAATTGGAAAATGCTCACTCCCTGGAGGATACACCCTGCCCTTTT TTATTTAAATTTCATCTTCATTGAATATACTTGTCTTTACCAACAGACTGTCCCCCCACG TATACCGTTTATCCCAATCTATTTGGGCCTATTTCCTTCCGTAAAAAGCTTCCCAGCCTT CCCCACCTTTAGTATCAGCGCGAGGCCTCGGCAATTCTTCAAATTCGTTCAGCCCCCTCC CTCTGCAACCCCCAATCCCTATCGCCCATATGCCCTTATAAATCCCCACGGCGGTTGCGC CCTTCAATTATTTAACCCCAATATNTTACCAAACCCTAACTCTCCTACGGTTTCCCCCCC CCCATCGTTCCCTCACAACCAATCT
Restriction Sites	NotI-NotI
ACCN	NM_020977
Insert Size	7680 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_020977.2, NP_066187.2
RefSeq Size	7911 bp
RefSeq ORF	5619 bp
Locus ID	287
UniProt ID	Q01484
Domains	DEATH, ZU5, ANK
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Isoform 2 is the major form of ankyrin in adult brain.

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