Ankyrin brain (ANK2) (NM_020977) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ANK-2; brank-2; CFAP87; FAP87; LQT4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC214181 representing NM_020977 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAACGAAGATGCAGCTCAGAAAAGCGACAGTGGAGAGAAGTTCAACGGCAGTAGTCAGAGGAGAA AAAGACCCAAGAAGTCTGACAGCAATGCAAGCTTCCTCCGTGCTGCCAGAGCAGGCAACCTGGACAAAGT TGTGGAATATCTGAAGGGGGGCATAGACATCAATACCTGCAATCAGAATGGACTCAACGCTCTCCATCTG GCTGCCAAGGAAGGCCACGTGGGGCTGGTGCAGGAGCTGCTGGGAAGAGGGTCCTCTGTGGATTCTGCCA CTAAGAAGGGAAATACCGCTCTTCACATTGCATCTTTGGCTGGACAAGCAGAAGTTGTCAAAGTTCTTGT TAAGGAAGGAGCCAATATTAATGCACAGTCTCAGAATGGCTTTACTCCTTTATACATGGCTGCCCAAGAG AATCACATTGATGTTGTAAAATATTTGCTGGAAAATGGAGCTAATCAGAGCACTGCTACAGAGGATGGCT TTACTCCTCTAGCTGTGGCACTCCAGCAAGGACACAACCAGGCGGTGGCCATCCTCTTGGAGAATGACAC CAAAGGGAAAGTGAGGCTGCCAGCTCTGCATATTGCCGCTAGGAAAGACGACACCAAATCTGCCGCACTT CTGCTTCAGAATGACCACAATGCTGACGTACAATCCAAGATGATGGTGAATAGGACAACTGAGAGTGGTT TTACCCCTTTGCACATAGCTGCACATTACGGAAATGTCAACGTGGCAACTCTTCTTCTAAACCGGGGAGC TGCTGTGGACTTCACAGCCAGGAATGGAATCACTCCTCTGCATGTGGCTTCCAAAAGAGGAAATACAAAC ATGGTGAAGCTCTTACTGGATCGAGGCGGTCAGATCGATGCCAAAACTAGGGATGGGTTGACACCACTTC ACTGTGCTGCACGAAGTGGGCATGACCAAGTGGTGGAACTTCTGTTGGAACGGGGTGCCCCCTTGCTGGC AAGGACTAAGAATGGGCTGTCTCCACTACACATGGCTGCCCAGGGAGACCACGTGGAATGTGTGAAGCAC CTGTTACAGCACAAGGCACCTGTTGATGATGTCACCCTAGACTACCTGACAGCCCTCCACGTTGCTGCGC ACTGTGGCCACTACCGTGTAACCAAACTCCTTTTAGACAAGAGAGCCAATCCGAACGCCAGAGCCCTGAA TGGTTTTACTCCACTGCACATTGCCTGCAAGAAAAACCGCATCAAAGTCATGGAACTGCTGGTGAAATAT GGGGCTTCAATCCAAGCTATAACAGAGTCTGGCCTCACACCAATACATGTGGCTGCCTTCATGGGCCACT TGAACATTGTCCTCCTTCTGCTGCAGAACGGAGCCTCTCCAGATGTCACTAACATTCGTGGTGAGACGGC ACTACACATGGCAGCCCGAGCCGGGCAGGTGGAAGTGGTCCGATGCCTCCTGAGAAATGGTGCCCTTGTT GATGCCAGAGCCAGGGAGGAACAGACACCTTTACATATTGCCTCCCGCCTGGGTAAGACAGAAATTGTCC AGCTGCTTCTACAACATATGGCTCATCCAGATGCGGCCACTACAAATGGGTACACACCACTGCACATCTC TGCCCGGGAGGGCCAGGTGGATGTGGCATCAGTCCTATTGGAAGCAGGAGCAGCCCACTCCTTAGCTACC AAGAAGGGTTTTACTCCCCTGCATGTAGCAGCCAAGTATGGAAGCCTGGATGTGGCAAAACTTCTCTTGC AACGCCGTGCTGCCGCAGATTCTGCAGGGAAGAACGGCCTTACCCCGCTCCATGTTGCTGCTCATTATGA CAACCAGAAGGTGGCGCTGCTGTTACTGGAGAAGGGTGCTTCCCCTCATGCCACTGCCAAGAATGGCTAT ACTCCGTTACATATTGCTGCCAAGAAGAATCAAATGCAGATAGCTTCCACACTCCTGAACTATGGAGCAG AGACAAACATTGTGACAAAGCAAGGAGTAACTCCACTCCATCTGGCCTCGCAGGAGGGGCACACAGATAT GGTTACCTTGCTTCTGGATAAGGGAGCCAATATCCACATGTCAACTAAGAGTGGACTCACATCCTTACAC CTTGCAGCCCAGGAAGATAAAGTGAATGTTGCTGATATTCTCACCAAGCATGGAGCTGATCAGGATGCTC ATACAAAGCTTGGTTACACACCTTTAATTGTGGCCTGTCACTATGGAAATGTGAAAATGGTCAACTTTCT TCTGAAGCAGGGAGCAAATGTTAACGCAAAAACCAAGAACGGCTACACGCCTTTGCACCAGGCCGCTCAG CAGGGTCACACGCACATCATCAACGTCCTGCTCCAGCATGGGGCCAAGCCCAACGCCACCACTGCGAATG GCAACACTGCCTTGGCGATTGCTAAGCGTCTGGGCTACATCTCCGTGGTCGACACCCTGAAGGTTGTGAC TGAGGAGGTCACCACCACCACCACAACTATTACAGAAAAACACAAACTAAATGTACCTGAGACGATGACT GAGGTTCTTGATGTTTCTGATGAAGAGGGTGATGACACAATGACTGGTGATGGGGGAGAATACCTTAGGC CTGAGGACCTAAAAGAACTGGGTGATGACTCACTACCCAGCAGTCAGTTCCTGGATGGTATGAATTACCT GCGATACAGCTTGGAGGGAGGACGATCTGACAGCCTTCGATCCTTCAGTTCCGACAGGTCTCACACTCTG AGCCATGCCTCCTACCTGAGGGACAGTGCCGTGATGGATGACTCAGTTGTGATTCCCAGTCACCAGGTGT CAACTCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGCCTAAGCTGGGGCACTGAGAACTTAGACAACGT GGCTCTTTCTTCTAGTCCTATTCATTCAGGTTTCCTGGTTAGTTTTATGGTGGATGCCCGAGGTGGTGCT ATGCGAGGATGCAGACACAATGGGCTCCGAATCATTATTCCACCTCGGAAATGTACTGCTCCAACGCGAG TCACCTGCCGACTGGTCAAGCGCCACAGACTGGCAACAATGCCTCCAATGGTGGAAGGAGAAGGCCTGGC CAGTCGCCTGATCGAAGTTGGACCTTCTGGTGCTCAGTTCCTTGGTAAACTTCACCTGCCAACGGCTCCT CCCCCACTTAATGAGGGAGAAAGTTTGGTCAGCCGCATTCTTCAGCTGGGGCCTCCTGGAACCAAATTCC TTGGGCCTGTGATCGTGGAGATCCCTCACTTTGCGGCCCTTCGAGGAAAGGAAAGGGAACTGGTGGTCCT GCGCAGTGAGAATGGGGACAGCTGGAAAGAGCATTTCTGTGACTACACTGAAGATGAATTGAATGAAATT CTTAACGGCATGGATGAAGTACTGGATAGCCCAGAAGACCTAGAAAAGAAACGAATCTGCCGCATCATCA CCCGAGACTTCCCACAGTACTTTGCAGTGGTGTCTCGTATCAAACAGGACAGCAATCTGATTGGCCCAGA AGGAGGTGTACTGAGCAGCACAGTGGTGCCCCAGGTGCAGGCCGTCTTCCCAGAGGGGGCACTCACCAAG CGGATCCGCGTAGGCCTGCAGGCTCAACCTATGCACAGTGAGCTGGTTAAGAAGATCCTAGGCAACAAAG CTACCTTCAGCCCTATAGTCACTTTGGAACCTAGAAGAAGAAAATTCCACAAACCAATTACCATGACCAT TCCTGTCCCCAAAGCTTCAAGTGATGTCATGTTGAATGGTTTTGGGGGAGATGCACCAACCTTAAGATTA CTATGCAGCATAACAGGTGGAACCACCCCTGCCCAGTGGGAAGATATTACAGGAACTACGCCATTAACAT TTGTCAATGAATGTGTTTCCTTTACAACAAACGTGTCTGCCAGGTTCTGGCTGATAGATTGTCGACAGAT CCAGGAATCCGTTACTTTTGCATCACAAGTATACAGAGAAATTATCTGCGTACCTTATATGGCCAAATTT GTAGTGTTTGCCAAATCACATGACCCCATTGAAGCCAGGTTGAGGTGTTTCTGCATGACTGATGATAAAG TGGATAAGACCCTTGAACAACAAGAAAATTTTGCTGAGGTGGCCAGAAGCAGGGATGTGGAGGTGTTAGA AGGAAAACCCATCTACGTTGATTGTTTCGGCAACTTGGTACCATTAACTAAAAGTGGCCAGCATCATATA TTCAGTTTTTTTGCCTTCAAAGAAAATAGACTTCCTCTATTTGTCAAGGTACGCGATACGACTCAGGAAC CTTGCGGACGACTATCATTTATGAAGGAGCCAAAATCCACGAGAGGCCTGGTGCATCAAGCTATTTGCAA CTTAAACATCACTTTGCCGATTTATACAAAGGAATCAGAGTCAGATCAAGAACAGGAGGAAGAGATCGAT ATGACATCAGAAAAAAATCCACAGGATGAGCAGGAACGGATCGAGGAAAGGCTGGCTTATATTGCTGATC ACCTTGGCTTCAGCTGGACAGAATTAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCATCAAATTCG AATTGAAAATCCCAACTCTCTTCAAGACCAGAGTCATGCACTGTTGAAGTACTGGCTAGAGAGGGATGGG AAACATGCTACAGATACCAACCTCGTTGAATGTCTCACCAAGATCAACCGAATGGATATTGTTCATCTCA TGGAGACCAACACAGAACCTCTCCAGGAGCGCATCAGTCATAGTTATGCAGAAATTGAACAGACCATTAC ACTGGATCATAGTGAAGGGTTCTCGGTACTTCAAGAGGAGTTATGCACTGCACAGCACAAGCAGAAAGAG GAGCAAGCTGTTTCTAAAGAAAGTGAGACCTGCGATCACCCTCCTATCGTCTCAGAGGAAGACATTTCTG TTGGTTATTCCACTTTTCAGGATGGCGTCCCCAAAACTGAGGGGGACAGCTCAGCAACAGCACTCTTTCC CCAAACTCACAAGGAGCAAGTTCAACAGGATTTCTCAGGGAAAATGCAAGACCTGCCTGAAGAGTCATCT CTGGAATATCAGCAGGAATATTTTGTGACAACTCCAGGAACAGAAACATCAGAGACTCAGAAGGCTATGA TAGTACCCAGCTCTCCCAGCAAGACACCTGAGGAAGTTAGCACCCCTGCAGAGGAGGAGAAGCTGTACCT CCAGACCCCAACATCCAGCGAGCGGGGAGGCTCTCCCATCATACAAGAACCCGAAGAGCCCTCAGAGCAC AGAGAGGAGAGCTCTCCGCGGAAAACCAGCCTCGTAATAGTGGAGTCTGCCGATAACCAGCCTGAGACCT GTGAAAGACTCGATGAAGATGCAGCTTTTGAAAAGGGAGACGATATGCCTGAAATACCCCCAGAAACAGT CACAGAAGAAGAATACATTGATGAGCATGGACACACCGTGGTAAAGAAGGTTACTAGGAAAATCATTAGG CGGTATGTATCCTCTGAAGGCACAGAGAAAGAAGAGATTATGGTGCAGGGAATGCCACAGGAACCTGTCA ACATCGAGGAAGGGGATGGCTATTCCAAAGTTATAAAGCGTGTTGTATTGAAGAGTGACACCGAGCAGTC AGAGGACAACAATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC214181 representing NM_020977 Red=Cloning site Green=Tags(s) MMNEDAAQKSDSGEKFNGSSQRRKRPKKSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHL AAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQE NHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAAL LLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTN MVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKH LLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKY GASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALV DARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLAT KKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGY TPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLH LAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQ QGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMT EVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTL SHASYLRDSAVMDDSVVIPSHQVSTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARGGA MRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGKLHLPTAP PPLNEGESLVSRILQLGPPGTKFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHFCDYTEDELNEI LNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTK RIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRL LCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKF VVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHI FSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEID MTSEKNPQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDG KHATDTNLVECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKE EQAVSKESETCDHPPIVSEEDISVGYSTFQDGVPKTEGDSSATALFPQTHKEQVQQDFSGKMQDLPEESS LEYQQEYFVTTPGTETSETQKAMIVPSSPSKTPEEVSTPAEEEKLYLQTPTSSERGGSPIIQEPEEPSEH REESSPRKTSLVIVESADNQPETCERLDEDAAFEKGDDMPEIPPETVTEEEYIDEHGHTVVKKVTRKIIR RYVSSEGTEKEEIMVQGMPQEPVNIEEGDGYSKVIKRVVLKSDTEQSEDNNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_020977
ORF Size	5616 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020977.4
RefSeq Size	7911 bp
RefSeq ORF	5619 bp
Locus ID	287
UniProt ID	Q01484
Domains	DEATH, ZU5, ANK
Protein Families	Druggable Genome
MW	205.6 kDa
Gene Summary	This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Dec 2011]

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