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ALKBH5 (NM_017758) Human Untagged Clone
CAT＃: SC127124
ALKBH5 (untagged)-Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5)
CNY 3,656.00
CNY 6,370.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ABH5; OFOXD; OFOXD1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127124 sequence for NM_017758 edited (data generated by NextGen Sequencing)
ATGGCGGCCGCCAGCGGCTACACGGACCTGCGTGAGAAGCTCAAGTCCATGACGTCCCGG GACAACTATAAGGCGGGCAGCCGGGAGGCCGCCGCCGCTGCCGCAGCCGCCGTAGCCGCC GCAGCCGCAGCCGCCGCTGCCGCCGAACCTTACCCTGTGTCCGGGGCCAAGCGCAAGTAT CAGGAGGACTCGGACCCCGAGCGCAGCGACTATGAGGAGCAGCAGCTGCAGAAGGAGGAG GAGGCGCGCAAGGTGAAGAGCGGCATCCGCCAGATGCGCCTCTTCAGCCAGGACGAGTGC GCCAAGATCGAGGCCCGCATTGACGAGGTGGTGTCCCGCGCTGAGAAGGGCCTGTACAAC GAGCACACGGTGGACCGGGCCCCACTGCGCAACAAGTACTTCTTCGGCGAAGGCTACACT TACGGCGCCCAGCTGCAGAAGCGCGGGCCCGGCCAGGAGCGCCTCTACCCGCCGGGCGAC GTGGACGAGATCCCCGAGTGGGTGCACCAGCTGGTGATCCAAAAGCTGGTGGAGCACCGC GTCATCCCCGAGGGCTTCGTCAACAGCGCCGTCATCAACGACTACCAGCCCGGCGGCTGC ATCGTGTCTCACGTGGACCCCATCCACATCTTCGAGCGCCCCATCGTGTCCGTGTCCTTC TTTAGCGACTCTGCGCTGTGCTTCGGCTGCAAGTTCCAGTTCAAGCCTATTCGGGTGTCG GAACCAGTGCTTTCCCTGCCGGTGCGCAGGGGAAGCGTGACTGTGCTCAGTGGATATGCT GCTGATGAAATCACTCACTGCATACGGCCTCAGGACATCAAGGAGCGCCGAGCAGTCATC ATCCTCAGGAAGACAAGATTAGATGCACCCCGGTTGGAAACAAAGTCCCTGAGCAGCTCC GTGTTACCACCCAGCTATGCTTCAGATCGCCTGTCAGGAAACAACAGGGACCCTGCTCTG AAACCCAAGCGGTCCCACCGCAAGGCAGACCCTGATGCTGCCCACAGGCCACGGATCCTG GAGATGGACAAGGAAGAGAACCGGCGCTCGGTGCTGCTGCCCACACACCGGCGGAGGGGT AGCTTCAGCTCTGAGAACTACTGGCGCAAGTCATACGAGTCCTCAGAGGACTGCTCTGAG GCAGCAGGCAGCCCTGCCCGAAAGGTGAAGATGCGGCGGCACTGA Clone variation with respect to NM_017758.3 >OriGene 5' read for NM_017758 unedited
GGATTTGTATACGACTTCTATAGGCGGCCGCGAATTCGGCACGAGGGGCCCCGGGGCGCG TCCCCTTAGAGCCATGCCCGGCTGCCCCGCCCGCCCCGGAGGACCCTAGAGCAGCGTCGT GGGGGCCATGGCGGCCGCCAGCGGCTACACGGACCTGCGTGAGAAGCTCAAGTCCATGAC GTCCCGGGACAACTATAAGGCGGGCATCTCGGGAGGCCGCCGCCGCTGCCGCAGCCGCCG TAGCCGCCGCAGCCGCAGCCGCCGCTGCCGCCGAACCTTACCCTGTGTCCGGGGCCAAGC GCAAGTATCAGGAGGACTCGGACCCCGAGCGCAGCGACTATGAGGAGCAGCAGCTGCAGA AGGAGGAGGAGGCGCGCAAGGTGAAGAGCGGCATCCGCCAGATGCGCCTCTTCAGCCAGG ACGAGTGCGCCAAGATCGAGGCCCGCATTGACGAGGTGGTGTCCCGCGCTGAGAAGGGCC TGTACAACGAGCACACGGTGGACCGGGCCCCACTGCGCAACAAGTACTTCTTCGGCGAAG GCTACACTTACGGCGCCCAGCTGCAGAAGCGCGGGCCCGGCCAGGAGCGCCTCTACCCGC CGGGCGACGTGGACGAGATCCCCGAGTGGGTGCACCAGCTGGTGATCCAAAAGCTGGTGG AGCACCGCGTCATCCTCGAGGGCTTCGTCAACAGCGCCGTCATCAACGACTACCACGCCC GCCGCCTGCATCGTGTCTCACGTGGACCCCATCCCCATCTTCGAGCGCCCCATCGTGTCC GTGTCCTTCTTTACCGACTCTGCGCTGTGCTTCNGNTGCAAGNTCCAGTCAAGCCTATTC GGGTGTCGGAACCAGTGCTTTCCCTGCCGGGCCCAAGGGAAACGTGACTGGGCTCACCGG TAAGCTGCTGAGAAATCCTCATGCATACGCCTCAGAACTAAGGACGCCCGCAGACCTATC TTCAGG >OriGene 3' read for NM_017758 unedited
NCCTTTAGCTCTGGACCGCGGCCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTAA AAATATTAGATTTGGTTTTTATTGTAGAGCACACATATCAGGGCGAAGTGCTGCACACAC AACTACAAATCATTTTTGGGAAAAAAGCTGTAAAAAAAATAAAACTGCAACTGCTTTAAT TATGCAGTCCCGACTTGAGAAATCCACGCTGGCAAAGGAGTTCTGGCCCCTGAGAGGGGC TTCTTGATGGTGGCTGCCCCAGATTTCTGCAGCCTATGAGGTCTCAGACCCGGACCTCTG TACACAAAGCCCCCAAAGTGGTGGTATCCTGGTTGCTGGGAGGGCCTCTGCTCTTGGCAA GCCCGGGTCCTGGCCTGGCTGACAAGAAGACATTCCTAATGAATCTAGATCCACAAAACC TTGCTGCCTTCCAGGATCTGAGTGGATAGAAGCTACCAAAGACTTTACCTTTTTTTTCTT ATTATTTTTTGAAAGCCCCTTCACCAACTCCCATACCCTGAAGCGGAGGAGGCACCAGCC CAGTCTGCAGGCCTGAGCTCTGGCCTTGGATGCATGCTGCCGGCAGACAAGATGGGGCAG GCGCCTTCCTAGAAGGATGACATGAGGATGAGGCCCAAGCTGGCCAGTGGAGAAGGGAAG GGAAAGGAAAAAGCCAGGAGACTAAAGGGGGAAAAAAAACTCAAAAAAGAACACAAAAAC CCTACCACACACCACAACAATTTTCCATACCCCCAATGCTAGACTTGGCAGGACACCAGT CCCTCTAGCTAGAAACATTTCTAAACTGACCCAACGTGGCAAGTCTAACTGTTCCCAATA ATCACACGCATATTGCTCAAAANAGCCACCAGCAAAAAGTGTGCTCTTGATAAGAAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017758 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017758.2, NP_060228.2 |
| RefSeq Size | 2992 bp |
| RefSeq ORF | 1377 bp |
| Locus ID | 54890 |
| UniProt ID | Q6P6C2 |
| Domains | 2OG-FeII_Oxy |
| Gene Summary | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3' UTR mRNAs in male germ cells (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209110 | ALKBH5 (Myc-DDK-tagged)-Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5) |
CNY 3,656.00 |
|
| RC209110L1 | Lenti ORF clone of Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209110L2 | Lenti ORF clone of Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5), mGFP tagged |
CNY 5,890.00 |
|
| RC209110L3 | Lenti ORF clone of Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209110L4 | Lenti ORF clone of Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5), mGFP tagged |
CNY 5,890.00 |
|
| RG209110 | ALKBH5 (tGFP-tagged) - Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5) |
CNY 5,256.00 |
