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ALKBH5 (NM_017758) Human Tagged ORF Clone
CAT＃: RC209110
ALKBH5 (Myc-DDK-tagged)-Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5)
ORF Plasmid: tGFP
"NM_017758" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
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CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ABH5; OFOXD; OFOXD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209110 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCCAGCGGCTACACGGACCTGCGTGAGAAGCTCAAGTCCATGACGTCCCGGGACAACTATA AGGCGGGCAGCCGGGAGGCCGCCGCCGCTGCCGCAGCCGCCGTAGCCGCCGCAGCCGCAGCCGCCGCTGC CGCCGAACCTTACCCTGTGTCCGGGGCCAAGCGCAAGTATCAGGAGGACTCGGACCCCGAGCGCAGCGAC TATGAGGAGCAGCAGCTGCAGAAGGAGGAGGAGGCGCGCAAGGTGAAGAGCGGCATCCGCCAGATGCGCC TCTTCAGCCAGGACGAGTGCGCCAAGATCGAGGCCCGCATTGACGAGGTGGTGTCCCGCGCTGAGAAGGG CCTGTACAACGAGCACACGGTGGACCGGGCCCCACTGCGCAACAAGTACTTCTTCGGCGAAGGCTACACT TACGGCGCCCAGCTGCAGAAGCGCGGGCCCGGCCAGGAGCGCCTCTACCCGCCGGGCGACGTGGACGAGA TCCCCGAGTGGGTGCACCAGCTGGTGATCCAAAAGCTGGTGGAGCACCGCGTCATCCCCGAGGGCTTCGT CAACAGCGCCGTCATCAACGACTACCAGCCCGGCGGCTGCATCGTGTCTCACGTGGACCCCATCCACATC TTCGAGCGCCCCATCGTGTCCGTGTCCTTCTTTAGCGACTCTGCGCTGTGCTTCGGCTGCAAGTTCCAGT TCAAGCCTATTCGGGTGTCGGAACCAGTGCTTTCCCTGCCGGTGCGCAGGGGAAGCGTGACTGTGCTCAG TGGATATGCTGCTGATGAAATCACTCACTGCATACGGCCTCAGGACATCAAGGAGCGCCGAGCAGTCATC ATCCTCAGGAAGACAAGATTAGATGCACCCCGGTTGGAAACAAAGTCCCTGAGCAGCTCCGTGTTACCAC CCAGCTATGCTTCAGATCGCCTGTCAGGAAACAACAGGGACCCTGCTCTGAAACCCAAGCGGTCCCACCG CAAGGCAGACCCTGATGCTGCCCACAGGCCACGGATTCTGGAGATGGACAAGGAAGAGAACCGGCGCTCG GTGCTGCTGCCCACACACCGGCGGAGGGGTAGCTTCAGCTCTGAGAACTACTGGCGCAAGTCATACGAGT CCTCAGAGGACTGCTCTGAGGCAGCAGGCAGCCCTGGGAACTCTGGCTCATCCTTACGTAGTTGCCCCTC CTTTTGTTTTGAGGGTTTTGTTTTTGTTCATTGGGGGGTTTTTGTTTTTTGTTTTTTGTTTTTTTTGATT CTATATATTTTTCCTTGGTTTTGTTGCCTGTTAGGGCTGAAGAATAGAATTGGCCAGGACCTAGGTTCTC ATATTCTTGGTATTCCTCCTGGATGGAAAGGCTGTTGGCATCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209110 protein sequence
Red=Cloning site Green=Tags(s) MAAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSD YEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYT YGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHI FERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVI ILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRS VLLPTHRRRGSFSSENYWRKSYESSEDCSEAAGSPGNSGSSLRSCPSFCFEGFVFVHWGVFVFCFLFFLI LYIFPWFCCLLGLKNRIGQDLGSHILGIPPGWKGCWHQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017758 |
| ORF Size | 1374 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017758.2, NP_060228.2 |
| RefSeq Size | 3449 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 54890 |
| UniProt ID | Q6P6C2 |
| Domains | 2OG-FeII_Oxy |
| MW | 51.4 kDa |
| Gene Summary | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3' UTR mRNAs in male germ cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209110L1 | Lenti ORF clone of Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209110L2 | Lenti ORF clone of Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5), mGFP tagged |
CNY 5,890.00 |
|
| RC209110L3 | Lenti ORF clone of Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209110L4 | Lenti ORF clone of Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5), mGFP tagged |
CNY 5,890.00 |
|
| RG209110 | ALKBH5 (tGFP-tagged) - Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5) |
CNY 5,256.00 |
|
| SC127124 | ALKBH5 (untagged)-Human alkB, alkylation repair homolog 5 (E. coli) (ALKBH5) |
CNY 3,656.00 |
