TPSAB1 (BC028059) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	lung tryptase; mast cell protease II; mast cell tryptase; MCP7; pituitary tryptase; skin tryptase; TPS1; TPS2; TPSB1; tryptase, alpha; tryptase-I; tryptase-III; tryptase 1; tryptase alpha/b; tryptase alpha II; tryptase beta 1; tryptase beta I; tryptase II
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC028059 edited GGAGTGGCCAGGATGCTGAGCCTGCTGCTGCTGGCGCTGCCCGTCCTGGCGAGCCCGGCC TACGCGGCCCCTGCCCCAGTCCAGGCCCTGCAGCAAGCGGGTATCGTCGGGGGTCAGGAG GCCCCCAGGAGCAAGTGGCCCTGGCAGGTGAGCCTGAGAGTCCGCGACCGATACTGGATG CACTTCTGTGGGGGCTCCCTCATCCACCCCCAGTGGGTGCTGACCGCGGCGCACTGCCTG GGACCGGACGTCAAGGATCTGGCCACCCTCAGGGTGCAACTGCGGGAGCAGCACCTCTAC TACCAGGACCAGCTGCTGCCGGTCAGCAGGATCATCGTGCACCCACAGTTCTACATCATC CAGACTGGAGCGGATATCGCCCTGCTGGAGCTGGAGGAGCCCGTGAACATCTCCAGCCGC GTCCACACGGTCATGCTGCCCCCTGCCTCGGAGACCTTCCCCCCGGGGATGCCGTGCTGG GTCACTGGCTGGGGCGATGTGGACAATGATGAGCCCCTCCCACCGCCATTTCCCCTGAAG CAGGTGAAGGTCCCCATAATGGAAAACCACATTTGTGACGCAAAATACCACCTTGGCGCC TACACGGGAGACGACGTCCGCATCATCCGTGACGACATGCTGTGTGCCGGGAACACCCGG AGGGACTCATGCCAGGGCGACTCTGGAGGGCCCCTGGTGTGCAAGGTGAATGGCACCTGG CTACAGGCGGGCGTGGTCAGCTGGGACGAGGGCTGTGCCCAGCCCAACCGGCCTGGCATC TACACCCGTGTCACCTACTACTTGGACTGGATCCACCACTATGTCCCCAAAAAGCCGTGA GTCAGGCCTGGGGTGTCCACCTGGGTCACTGGAGAGCCAGCCCCTCCTGTCCAAAACACC ACTGCTTCCTACCCAGGTGGCGACTGCCCCCCACACCTTCCCTGCCCCGTCCTGAGTGCC CCTTCCTGTCCTAAGCCCCCTGCTCTCTTCTGAGCCCCTTCCCCTGTCCTGAGGACCCTT CCCCATCCTGAGCCCCCTTCCCTGTCCTAAGCCTGACGCCTGCACTGGGCCCTCCGGCCC TCCCCTGCCCAGGCAGCTGGTGGTGGGCGCTAATCCTCCTGAGTGCTGGACCTCATTAAA GTGCATGGAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC028059 unedited CCCCGATCGGGATTTGTAATACGACTTTATATAGGCGGCCGCGNATTCANATCTGGTACC GGTCCGGAATTCCCGGGATGGAGTGGCCAGGATGCTGAGCCTGCTGCTGCTGGCGCTGCC CGTCCTGGCGAGCCCGGCCTACGCGGCCCCTGCCCCAGTCCAGGCCCTGCAGCAAGCGGG TATCGTCGGGGGTCAGGAGGCCCCCAGGAGCAAGTGGCCCTGGCAGGTGAGCCTGAGAGT CCGCGACCGATACTGGATGCACTTCTGTGGGGGCTCCCTCATCCACCCCCAGTGGGTGCT GACCGCGGCGCACTGCCTGGGACCGGACGTCAAGGATCTGGCCACCCTCAGGGTGCAACT GCGGGAGCAGCACCTCTACTACCAGGACCAGCTGCTGCCGGTCAGCAGGATCATCGTGCA CCCACAGTTCTACATCATCCAGACTGGAGCGGATATCGCCCTGCTGGAGCTGGAGGAGCC CGTGAACATCTCCAGCCGCGTCCACACGGTCATGCTGCCCCCTGCCTCGGAGACCTTCCC CCCGGGGATGCCGTGCTGGGTCACTGGCTGGGGCGATGTGGACAATGATGAGCCCCTCCC ACCGCCATTTCCCCTGAAGCAGGTGAAGGTCCCCATAATGGAAAACCACATTTGTGACGC AAAATACCACCTTGGCGCCTACACGGGAGACGACGTCCGCATCATCCGTGACGACATGCT GTGTGCCGGGAACACCCGGAGGGACTCATGCCAGGGCGACTCTGGAGGGCCCCTGGTGTG CAAGTGAATGGGACCTGGCTACAGGCGGGCGTGGTCAGCTGGGACGAGGCTGTGCCCAAC CCAACCGGCCTGGCATCTACACCCGGGTCACCTACTACCTGGGACTGTATCCCACCCT
Restriction Sites	Please inquire
ACCN	BC028059
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC028059.1, AAH28059.1
RefSeq Size	1206 bp
Locus ID	7177
Protein Families	Druggable Genome, Protease, Secreted Protein
Gene Summary	Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.