Cytokeratin 10 (KRT10) (NM_000421) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCIE; BIE; CK10; EHK; K10; KPP
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_000421 edited CAGCATCACCATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGG AGGAGGAGGAGGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTC TAGCAGCAAAGGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTT TAGCCGTGGGAGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGGTGGCTATGGAGGATT AGGAGGTTTTGGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGCAGCTTTGGTGGGAG TTATGGAGGCAGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGCAGCTT TGGTGGAGGCGGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGG AGATGGTGGCCTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCT GGCTTCCTACTTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAA AATCAAGGAGTGGTATGAAAAGCATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAG CAAATACTACAAAACCATCGATGACCTTAAAAATCAGATTCTCAACCTAACAACTGATAA TGCCAACATCCTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGATGACTTCAGGCTGAA GTATGAGAATGAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAACGGCCTGCGTAG GGTGCTGGATGAGCTGACCCTGACCAAGGCTGACCTGGAGATGCAAATTGAGAGCCTGAC TGAAGAGCTGGCCTATCTGAAGAAGAACCACGAGGAGGAAATGAAAGACCTTCGAAATGT GTCCACTGGTGATGTGAATGTGGAAATGAATGCTGCCCCGGGTGTTGATCTGACTCAACT TCTGAATAACATGAGAAGCCAATATGAACAACTTGCTGAACAAAACCGCAAAGATGCTGA AGCCTGGTTCAATGAAAAGAGCAAGGAACTGACTACAGAAATTGATAATAACATTGAACA GATATCCAGCTATAAATCTGAGATTACTGAATTGAGACGTAATGTACAAGCTCTGGAGAT AGAACTACAGTCCCAACTGGCCTTGAAACAATCCCTGGAAGCCTCCTTGGCAGAAACAGA AGGTCGCTACTGTGTGCAGCTCTCACAGATTCAGGCCCAGATATCCGCTCTGGAAGAACA GTTGCAACAGATTCGAGCTGAAACCGAGTGCCAGAATACTGAATACCAACAACTCCTGGA TATTAAGATCCGACTGGAGAATGAAATTCAAACCTACCGCAGCCTGCTAGAAGGAGAGGG AAGTTCCGGAGGCGGCGGACGCGGCGGCGGAAGTTTCGGCGGCGGCTACGGCGGCGGAAG CTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGCGGCGGCCACGGCGGCAGTTCCGGCGG CGGCTACGGAGGCGGAAGCTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGGGGCGGAAG CTCCAGCGGCGGCCACGGCGGCAGTTCCAGCGGCGGCTACGGTGGTGGCAGTTCCGGCGG CGGCGGCGGCGGCTACGGGGGCGGCAGCTCCGGCGGCGGCAGCAGCTCCGGCGGCGGATA CGGCGGCGGCAGCTCCAGCGGAGGCCACAAGTCCTCCTCTTCCGGGTCCGTGGGCGAGTC TTCATCTAAGGGACCAAGATACTAACAAAACCAGAGTAATCAAGACAATTATTGAAGAGG TGGCGCCCGACGGTAGAGTTCTTTCATCTATGGTTGAATCAGAAACCAAGAAACACTACT ATTAAACTGCATCAAGAGGAGAGAGTCTCCCTTCACACAGACCATTATTTACAGATGCAT GGAAAACAAAGTCTCCAAGAAAACACTTCTGTCTTGATGGTCTATGGAAATAGACCTTGA AAATAAGGTGTCTACAAGGTGTTTTGTGGTTTCTGTATTTCTTCTTTTCACTTTACCAGA AAGTGTTCTTTAATGGAAAGAAAAACAACTTTCTGTTCTCATTTACTAATGAATTTCAAT AAACTTTCTTACTGATGCAAACTAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_000421 unedited NGGGATTGGGGNNGAAAACCACNCTCTTAGGGCGGCCGCGATTCCCGGGATCAGCATCAC CATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGGAGGAGGAGG AGGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTCTAGCAGCAA AGGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTTTAGCCGTGG GAGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGGTGGCTATGGAGGATTAGGAGGTTT TGGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGCAGCTTTGGTGGGAGTTATGGAGG CAGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGCAGCTTTGGTGGAGG CGGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGGAGATGGTGG CCTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCTGGCTTCCTA CTTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAAAATCAAGGA GTGGTATGAAAAGCATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAGCAAATACTA CAAAACCATCGATGACCTTAAAAATCAGATTCTCAACCTAACAACTGATAATGCCAACAT CCTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGATGACTTCAGGCTGAAGTATGAGAA TGAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAACGGCCTGCGTANGGTGCTGGA TGAGCTGACCCCTGACCAAGCTGACCTGNAGATGCANATTGAGAGCCTGACTGNNAGAGC TGCCCTATCTGAGAAGAACCACGAGA
Restriction Sites	Please inquire
ACCN	NM_000421
Insert Size	2145 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_000421.2, NP_000412.2
RefSeq Size	2145 bp
RefSeq ORF	1755 bp
Locus ID	3858
UniProt ID	P13645
Gene Summary	This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

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