Cytokeratin 10 (KRT10) (NM_000421) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BCIE; BIE; CK10; EHK; K10; KPP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC204500 representing NM_000421. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGGAGGAGGAGGAGGAGGAGGA GGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTCTAGCAGCAAAGGCTCCCTTGGTGGAGGA TTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTTTAGCCGTGGGAGCTCTGGTGGGGGCTGCTTTGGGGGC TCATCAGGTGGCTATGGAGGATTAGGAGGTTTTGGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGC AGCTTTGGTGGGAGTTATGGAGGCAGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGC AGCTTTGGTGGAGGCGGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGGAGAT GGTGGCCTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCTGGCTTCCTACTTG GACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAAAATCAAGGAGTGGTATGAAAAG CATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAGCAAATACTACAAAACCATCGATGACCTTAAA AATCAGATTCTCAACCTAACAACTGATAATGCCAACATCCTGCTTCAGATCGACAATGCCAGGCTGGCA GCTGATGACTTCAGGCTGAAGTATGAGAATGAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAAC GGCCTGCGTAGGGTGCTGGATGAGCTGACCCTGACCAAGGCTGACCTGGAGATGCAAATTGAGAGCCTG ACTGAAGAGCTGGCCTATCTGAAGAAGAACCACGAGGAGGAAATGAAAGACCTTCGAAATGTGTCCACT GGTGATGTGAATGTGGAAATGAATGCTGCCCCGGGTGTTGATCTGACTCAACTTCTGAATAACATGAGA AGCCAATATGAACAACTTGCTGAACAAAACCGCAAAGATGCTGAAGCCTGGTTCAATGAAAAGAGCAAG GAACTGACTACAGAAATTGATAATAACATTGAACAGATATCCAGCTATAAATCTGAGATTACTGAATTG AGACGTAATGTACAAGCTCTGGAGATAGAACTACAGTCCCAACTGGCCTTGAAACAATCCCTGGAAGCC TCCTTGGCAGAAACAGAAGGTCGCTACTGTGTGCAGCTCTCACAGATTCAGGCCCAGATATCCGCTCTG GAAGAACAGTTGCAACAGATTCGAGCTGAAACCGAGTGCCAGAATACTGAATACCAACAACTCCTGGAT ATTAAGATCCGACTGGAGAATGAAATTCAAACCTACCGCAGCCTGCTAGAAGGAGAGGGAAGTTCCGGA GGCGGCGGACGCGGCGGCGGAAGTTTCGGCGGCGGCTACGGCGGCGGAAGCTCCGGCGGCGGAAGCTCC GGCGGCGGCTACGGCGGCGGCCACGGCGGCAGTTCCGGCGGCGGCTACGGAGGCGGAAGCTCCGGCGGC GGAAGCTCCGGCGGCGGCTACGGGGGCGGAAGCTCCAGCGGCGGCCACGGCGGCAGTTCCAGCGGCGGC TACGGTGGTGGCAGTTCCGGCGGCGGCGGCGGCGGCTACGGGGGCGGCAGCTCCGGCGGCGGCAGCAGC TCCGGCGGCGGATACGGCGGCGGCAGCTCCAGCGGAGGCCACAAGTCCTCCTCTTCCGGGTCCGTGGGC GAGTCTTCATCTAAGGGACCAAGATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC204500 Blue=ORF Red=Cloning site Green=Tag(s) MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRGSSGGGCFGG SSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGSFGGGSFGGGSFGGGSFGGGGFGGGGFGGGFGGGFGGD GGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKEWYEKHGNSHQGEPRDYSKYYKTIDDLK NQILNLTTDNANILLQIDNARLAADDFRLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESL TEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSK ELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISAL EEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSS GGGYGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSS SGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_000421
ORF Size	1752 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000421.2, NP_000412.2
RefSeq Size	2162 bp
RefSeq ORF	1755 bp
Locus ID	3858
UniProt ID	P13645
MW	58.8 kDa
Gene Summary	This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

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