Rubicon (RUBCN) (BC033615) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA0226; RUBICON; SCAR15
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC033615 edited GGCCGGGATAGAAGCCGCAGCCCAGGCGGCGGTCCCTCCCGCTGTCGCCGCCCGGGTGTA GCGCATCACCCGGAGCCCACCGGCCGCAGGTGCCTCCTCCGGCCCCAGGGGGCCCCGGGA GCCCTGAAGGGCGAAGCGGCAGGGACGCCTCTCTTGGGCGAAGAGGCGGCCTCACCGCCC CGGATGCGGCCGGAGGGCGCGGGAATGGAGCTCGGAGGCGGCGAGGAGCGCCTGCCTGAG GAGAGCAGGAGGGAGCACTGGCAGTTGCTGGGTAATTTGAAGACGACGGTGGAGGGTTTG GTATCAACCAACAGCCCCAACGTCTGGTCTAAGTATGGTGGCTTGGAGCGGCTTTGCAGG GACATGCAGAGCATCCTCTATCACGGGCTTATCCGTGACCAGGCGTGCCGCCGCCAGACG GATTACTGGCAGTTCGTGAAAGACATCCGGTGGCTCAGTCCCCACTCAGCCCTTCACGTG GAGAAGTTCATCAGCGTGCACGAGAACGACCAGAGCAGTGCTGATGGTGCCAGTGAACGT GCTGTTGCCGAGCTGTGGCTGCAGCACAGCCTGCAGTACCACTGCCTCTCAGCCCAGCTC CGGCCCCTGCTCGGGGATAGACAGTATATCAGAAAATTCTACACAGATGCTGCCTTCCTG CTAAGTGACGCTCATGTCACGGCCATGCTGCAGTGCCTGGAAGCAGTGGAACAGAACAAC CCCCGCCTCCTGGCTCAGATCGATGCGTCCATGTTTGCCAGAAAGCACGAGAGCCCGCTC CTGGTGACAAAGAGCCAGAGCCTGACAGCCCTGCCCAGTTCCACATACACCCCTCCAAAC AGCTATGCTCAGCATTCCTACTTTGGGTCCTTCTCTAGCCTCCACCAATCCGTGCCCAAC AATGGCTCAGAGAGAAGATCTACTTCCTTTCCACTCTCTGGCCCTCCCCGGAAACCTCAA GAAAGCAGAGGGCACGTCTCACCAGCAGAGGATCAAACCATCCAAGCCCCCCCAGTTTCA GTCTCTGCACTAGCCAGGGATTCCCCTTTGACCCCAAATGAAATGAGCTCCAGTACTCTG ACCAGCCCCATAGAGGCATCCTGGGTCAGCAGCCAGAATGATTCCCCAGGTGATGCCAGT GAGGGGCCTGAGTACCTGGCCATTGGCAACTTGGACCCCCGAGGCCGGACTGCCAGCTGT CAGAGTCACAGCAGCAATGCCGAGAGCAGCAGTTCCAATTTGTTCTCCTCCAGCAGCTCC CAGAAGCCAGATTCTGCTGCCTCTTCCTTAGGGGACCAGGAAGGAGGTGGGGAGAGCCAG CTGTCCAGTGTCCTCCGCAGGTCCAGCTTCTCAGAGGGGCAGACACTCACTGTCACCAGT GGGGCAAAGAAAAGCCACATTCGCTCCCATTCGGATACCAGCATTGCCTCCAGGGGAGCT CCAGGTAATGAGGAACACCGGCTGCTAGTTAGCAAGATGACCCTGAATTGAGAGTAAGAG AGGGCTTGTTATGGGGACTTTTCAGGTGGCAGAAAGAGATGTTCTGTTTCTGGTGATACA ACATCTTATTTTGTAATGAAAGAAGTTTATAACGAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033615 unedited NGGTGAACCGTTCAGNAAATTTTGTNAATACGNACTNCACTNANAGGGNNCGGCCGCGNA ATTCCCGGGATGGCCGGGATAGAAGCCGCAGCCCAGGCGGCGGTCCCTCCCGCTGTCGCC GCCCGGGTGTAGCGCATCACCCGGAGCCCACCGGCCGCAGGTGCCTCCTCCGGCCCCAGG GGGCCCCGGGAGCCCTGAAGGGCGAAGCGGCAGGGACGCCTCTCTTGGGCGAAGAGGCGG CCTCACCGCCCCGGATGCGGCCGGAGGGCGCGGGAATGGAGCTCGGAGGCGGCGAGGAGC GCCTGCCTGAGGAGAGCAGGAGGGAGCACTGGCAGTTGCTGGGTAATTTGAAGACGACGG TGGAGGGTTTGGTATCAACCAACAGCCCCAACGTCTGGTCTAAGTATGGTGGCTTGGAGC GGCTTTGCAGGGACATGCAGAGCATCCTCTATCACGGGCTTATCCGTGACCAGGCGTGCC GCCGCCAGACGGATTACTGGCAGTTCGTGAAAGACATCCGGTGGCTCAGTCCCCACTCAG CCCTTCACGTGGAGAAGTTCATCAGCGTGCACGAGAACGACCAGAGCAGTGCTGATGGTG CCAGTGAACGTGCTGTTGCCGAGCTGTGGCTGCAGCACAGCCTGCAGTACCACTGCCTCT CAGCCCAGCTCCGGCCCCTGCTTCGGGATAGACAGTATATCAGAAAATTCTACACAGATG CTGCCTTCCTGCTAAGTGACGCTTCATGTCAACGCCATGCTTGCATGCCTGGGAGCATTG GAAACAGACAACCCCCGCCTCCGGGCTAAAATCAATGCTCCCATGTTTGCCAAAAAGCCC GAGAGCCTGCTCCTGGTAACAAAAGCCCGAGCCGACAGTCCTGCCCAGTTTCCATT
Restriction Sites	Please inquire
ACCN	BC033615
Insert Size	1730 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033615.1, AAH33615.1
RefSeq Size	1730 bp
Locus ID	9711
Gene Summary	The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

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